In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)

İbrahim KOÇ, (Gebze Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Gebze, Kocaeli, Türkiye)
Ahmet ONAY, (Dicle Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Diyarbakır, Türkiye)
Yelda ÖZDEN ÇİFTÇİ (Gebze Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Gebze, Kocaeli, Türkiye)
Turkish Journal of Biology
9 0
Yıl: 2014 Cilt: 38 Sayı: 5 Sayfa Aralığı: 653 - 663 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)

Öz:
Pistacia lentiscus L. (lentisk or mastic tree) is an economically important member of the genus Pistacia due to its valuable mastic resin. Shoot tips and nodal segments were used as explant sources from in vitro-germinated seeds of lentisk. Shoot tips were found to be suitable for multiple shoot formation. Thereafter, the influences of different growth regulators [N6-benzyladenine (BA), gibberellic acid (GA3), naphthalene acetic acid (NAA), or jasmonic acid (JA)] together with various elicitors [silver nitrate (AgNO3) or phloroglucinol (PG)] were assessed to develop efficient micropropagation protocol. Our results showed that the combination of all tested concentrations of GA3 with 1.0 mg/L BA resulted in enhancement of multiple shoot formation. The maximum number of shoots per explant (3.95) was recorded on MS medium containing 1.0 mg/L BA and 0.3 mg/L GA3. In contrast, JA had a negative influence, while AgNO3 had no significant effect on multiple shoot formation. In terms of synthetic seed production, it was possible to encapsulate shoot tips at 4 °C in darkness for up to 6 months with a frequency of 87.5% plant regrowth. In vitro-propagated microshoots, including plantlets derived from synthetic seeds, were transferred to MS medium containing different concentrations (1.0, 2.0, or 4.0 mg/L) of indole butyric acid for rooting and successfully acclimatized to ex vitro conditions. The presented data suggest an efficient in vitro regeneration system and conservation via synthetic seed production for lentisk.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Abu-Qaoud H (2007). Effect of scarification, gibberellic acid and stratification on seed germination of three Pistacia species. An- Najah Univ J Res (N Sc) 12: 1–11.
  • Acar İ (1988). (Pistacia lentiscus L . var. Chia) Sakızı Üretiminin Geliştirilmesine Esas Olmak Üzere Sakızın Fiziko-kimyasal Yönden İncelenmesi. Ankara, Turkey: Forestry Research Institute Technical Report No: 35 (in Turkish).
  • Ak BE, Parlakcı H (2009). Pistacia lentiscus in the Mediterranean Region in Turkey. Acta Hort 818: 77–81.
  • Arya S, Sharma S, Kaur R, Arya ID (1999). Micropropagation of Dendrocalamus asper by shoot proliferation using seeds. Plant Cell Rep 18: 879–882.
  • Balan KV, Prince J, Han Z, Dimas K, Cladaras M, Wyche GH, Sitaras NM, Pantazis P (2007). Antiproliferative activity and induction of apoptosis in human colon cancer cells treated in vitro with constituents of a product derived from Pistacia lentiscus L. var. chia. Phyto Med 14: 263–272.
  • Baytop T (1968). Türkiye’de sakız (mastix) elde etme imkanları. İstanbul Üniversitesi Eczacılık Fakültesi Mecmuası 4: 31–35 (in Turkish).
  • Boggetti B, Jasik J, Mantell S (1999). In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse raised plants. Plant Cell Rep 18: 456–461.
  • Caboni E, D’Angeli S, Chiappetta A, Innocenti AM, Van Onckelen H, Damiano C (2002). Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process. Plant Cell Tiss Organ Cult 70: 199–206.
  • Calabro G, Curro P (1974). Costituenti degli oliessenziali Nota IV. Essenza di lentisco. Esence Deriv Agrum 44: 82–92 (in Italian).
  • Capitani F, Biondi S, Falasca G, Ziosi V, Balestrazzi A, Carbonera D, Torrigiani P, Altamura MM (2005). Methyl jasmonate disrupts shoot formation in tobacco thin cell layers by over-inducing mitotic activity and cell expansion. Planta 220: 507–519.
  • Castola V, Bighelli A, Casanova J (2000). Intraspecific chemical variability of the essential oil of Pistacia lentiscus L. from Corsica. Biochem Syst Ecol 28: 79–88.
  • Chandra R, Khetarpal S, Polisetty R (1997). Effect of plant growth regulators on evolution of ethylene and methane by different explants of chickpea. Biol Plantarum 40: 337–343.
  • Correia OA, Catarino FM (1994). Seasonal changes in soil-to-leaf resistance in Cistus sp. and Pistacia lentiscus. Acta Oecol 15: 289–300.
  • Dabos KJ, Sfika E, Vlatta LJ, Frantzi D, Amygdalos GI, Giannikopoulos G (2010). Is Chios mastic gum effective in the treatment of functional dyspepsia? A prospective randomised double-blind placebo controlled trial. J Ethnopharmacol 127: 205–209.
  • Dogan Y, Baslar S, Aydin H, Mert HH (2003). A study of the soil- plant interactions of Pistacia lentiscus L. distributed in the western Anatolian part of Turkey. Acta Bot Croat 62: 73–88.
  • Dolcet-Sanjuan R, Claveria E (1995). Improved shoot-tip micropropagation of Pistacia vera L. and the beneficial effects of methyl jasmonate. J Amer Soc Hort Sci 120: 938–942.
  • Fascella G, Airò M, Zizzo GV, Ruffoni B (2004). Prime osservazioni sulla coltivazione in vitro di Lentisco (Pistacia lentiscus L.). Italus Hortus 11: 141–143 (in Italian).
  • Gardeli C, Vassiliki P, Athanasios M, Kibouris T, Michael K (2008). Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem 107: 1120–1130.
  • Gaspar T, Kevers C, Penel C, Greppin H, Reid MD, Thorpe AT (1996). Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cell Dev Biol Plant 32: 272–289.
  • Grundwag M (1976). Embryology and fruit development in four species of Pistacia (Anacardiaceae). Bot J Linn Soc 73: 355–370.
  • Hossain SN, Munshi MK, Islam MR, Hakim L, Hossain M (2003). In vitro propagation of plum (Zyziphus jujuba Lam.). Plant Tissue Cult 13: 81–84.
  • Isfendiyaroglu M (2000). Cutting propagation of mastic tree (Pistacia lentiscus var. Chia Duham.). NUCIS Newsletter 9: 42–44.
  • Jarda L, Butiuc-Keul A, Höhn M, Pedryc A, Cristea V (2014). Ex situ conservation of Dianthus giganteus d’Uvr. subs. Banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turk J Biol 38: 21–30.
  • Kim HJ, Neophytou C (2009). Natural anti-inflammatory compounds for the management and adjuvant therapy of inflammatory bowel disease and its drug delivery system. Arch Pharm Res 32: 997–1004.
  • Koroch AR, Kapteyn J, Juliani HR, Simon JE (2003). In vitro regeneration of Echinacea pallida from leaf explants. In Vitro Cell Dev Biol Plant 39: 415–418.
  • Ladd PG, Crosti R, Pignatti S (2005). Vegetative and seedling regeneration after fire in planted Sardinian pinewood compared with that in other areas of Mediterranean-type climate. J Biogeogr 32: 85–98.
  • Lambardi M, Sharma KK, Thorpe TA (1993). Optimization of in vitro bud induction and plantlet formation from mature embryos of Aleppo pine (Pinus halepensis Mill.). In Vitro Cell Dev Biol Plant 29: 189–199.
  • Lemonakis N, Magiatis P, Kostomitsopoulos N, Skaltsounis A, Tamvakopoulos C (2011). Oral administration of Chios mastic gum or extracts in mice: quantification of triterpenic acids by liquid chromatography-tandem mass spectrometry. Planta Med 77: 1916–1923.
  • Ljubuncic P, Azaizeh H, Portnaya I, Coganc U, Said O (2005a). Antioxidant activity and cytotoxicity of eight plants used in traditional Arab medicine in Israel. J Ethnopharmacol 99: 43–47.
  • Ljubuncic P, Song H, Cogan U, Azaizeh H, Bomzon A (2005b). The effects of aqueous extracts prepared from the leaves of Pistacia lentiscus in experimental liver disease. J Ethnopharmacol 100: 198–204.
  • Magiatis P, Melliou E, Skaltsounid AL, Chinou IB, Mitaku S (1999). Chemical composition and antimicrobial activity of the essential oils of Pistacia lentiscus var. chia. Plant Med 65: 749–752.
  • Marascuilo LA, McSweeney M (1977). Post-hoc multiple comparisons in sample preparations for test of homogenesity. In: McSweeney M, Marascuilo LA, editors. Nonparametric and Distribution- Free Methods for the Social Sciences. Belmont, CA, USA: Books/Cole Publication, pp. 141–147.
  • Martin MT, Pedranzani H, García-Molinero P, Pando V, Sierra-De- Grado R (2009). Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait. Biocell 33: 141–148.
  • Mascarello C, Fascella G, Zizzo GV, Mantovani E, Ruffoni B (2007). In vivo and in vitro Propagation of Pistacia lentiscus L. Acta Hortic 764: 354–361.
  • Moubayidin L, Di Mambro R, Sabatini S (2009). Cytokinin–auxin crosstalk. Trends Plant Sci 14: 557–562.
  • Mulas M, Abeltino P, Brigaglia N (1998). Evaluation of Pistacia lentiscus L. genetic resources to select ecotypes having high efficiency in the colonisation of marginal lands. Acta Hortic 457: 279–286.
  • Murashige T, Skoog FA (1962). Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473–479.
  • Nhut DT, Hai NT, Phan MX (2010). A highly efficient protocol for micropropagation of Begonia tuberous. In: Jain SM, Ochatt SJ, editors. Protocols for In Vitro Propagation of Ornamental Plants. New York, NY, USA: Springer, pp. 15–20.
  • Ozden-Tokatli Y, Ozudogru EA, Akcin A (2005). In vitro response of pistachio nodal explants to silver nitrate. Sci Hortic 106: 415–426.
  • Paraschos S, Magiatis P, Mitakou S, Petraki K, Kalliaropoulos A, Maragkoudakis A, Mentis A, Sgouras D, Skaltsounis AL (2007). In vitro and in vivo activities of Chios mastic gum extracts and constituents against Helicobacter pylori. Antimicrob Agents Chemother 51: 551–559.
  • Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006). In vitro propagation of rose—a review. Biotech Adv 24: 94–114.
  • Ravnikar M, Zel J, Plaper I, Spacapan A (1993). Jasmonic acid stimulates shoot and bulb formation of garlic in vitro. J Plant Growth Regul 12: 73–77.
  • Rojo E, Solano R, Sanchez-Serrano JJ (2003). Interaction between signalling component involved in plant defences. Plant Growth Regul 22: 82–98.
  • Ruffoni B, Mascarello C, Fascella G, Airó M, Amoretti M (2004). La micro-propagazione del Lentisco (Pistacia lentiscus L.). Atti II° Convegnonazionale Piantemediterranee. Italus Hortus 11: 141–143 (in Italian).
  • Sharmin SA, Alam MJ, Sheikh MI, Zaman R, Khalekuzzaman M, Mondal SC, Haque MA, Alam MF, Alam I (2013). Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant. Turk J Biol 37: 698–708.
  • Shyamkumar B, Anjaneyulu C, Giri CC (2003). Multiple shoot induction from cotyledonary node explants of Terminalia chebula. Biol Plantarum 47: 585–588.
  • Tampe PA, Reid DM, Thorpe TA (2001). Jasmonic acid inhibition of in vitro shoot organogenesis in Pinus radiata cotyledons. J Plant Physiol 158: 607–611.
  • Taşkın T, İnal A (2005). The researches on in vitro micropropagation of mastic tree (Pistacia lentiscus var. chia Duhamel). Anadolu J of AARI 15: 1–15 (in Turkish with English abstract).
  • Teixeira da Silva J, Dobránszki J, Ross S (2013). Phloroglucinol in plant tissue culture. In Vitro Cell Dev Biol-Plant 49: 1–16.
  • Theodoropoulos H, Apostolopoulos CD (2004). The influence of producer’s characteristics on the prospects and productivity of mastic farms on the island of Chios, Greece. In: Proceedings of the First World Congress of Rural Sociology, 25–30 July 2004; Trondheim, Norway.
  • Tilkat E, Isikalan C, Onay A (2005). In vitro propagation of khinjuk pistachio (Pistacia khinjuk stocks) through seedling apical shoot tip culture. Propag Ornam Plants 5: 124–128.
  • Triantafyllou A, Bikineyeva A, Dikalova A, Nazarewicz R, Lerakis S, Dikalov S (2011). Anti-inflammatory activity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress. Nutr J 10: 1–9.
  • Tsvetkov I, Hausman JF (2004). In vitro regeneration from alginate- encapsulated microcuttings of Quercus sp. Sci Hortic 103: 503–507.
  • Vengadesan G, Pijut PM (2009). In vitro propagation of northern red oak (Quercus rubra L.). In Vitro Cell Dev Biol Plant 45: 474–482.
  • Vyas S, Joshi N, Tak K, Purohit SD (2005). In vitro adventitious shoot bud differentiation and plantlet regeneration in Feronia limonia L. (SWINGLE). In Vitro Cell Dev Biol Plant 41: 296–302.
  • Wasternack C, Hause B (2002). Jasmonates and octadecanoids: signals in plant stress responses and development. Prog Nucleic Acids Res Mol Biol 72: 165–221.
  • Yıldırım H (2012). Micropropagation of Pistacia lentiscus L. from axenic seedlings-derived explants. Sci Hortic 137: 29–35. Zohary M (1952). A monographic study of the genus Pistacia. Palest J Bot 5: 187–226.
APA koç i, ONAY A, ÖZDEN ÇİFTÇİ Y (2014). In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). , 653 - 663.
Chicago koç ibrahim,ONAY Ahmet,ÖZDEN ÇİFTÇİ Yelda In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). (2014): 653 - 663.
MLA koç ibrahim,ONAY Ahmet,ÖZDEN ÇİFTÇİ Yelda In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). , 2014, ss.653 - 663.
AMA koç i,ONAY A,ÖZDEN ÇİFTÇİ Y In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). . 2014; 653 - 663.
Vancouver koç i,ONAY A,ÖZDEN ÇİFTÇİ Y In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). . 2014; 653 - 663.
IEEE koç i,ONAY A,ÖZDEN ÇİFTÇİ Y "In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)." , ss.653 - 663, 2014.
ISNAD koç, ibrahim vd. "In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)". (2014), 653-663.
APA koç i, ONAY A, ÖZDEN ÇİFTÇİ Y (2014). In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turkish Journal of Biology, 38(5), 653 - 663.
Chicago koç ibrahim,ONAY Ahmet,ÖZDEN ÇİFTÇİ Yelda In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turkish Journal of Biology 38, no.5 (2014): 653 - 663.
MLA koç ibrahim,ONAY Ahmet,ÖZDEN ÇİFTÇİ Yelda In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turkish Journal of Biology, vol.38, no.5, 2014, ss.653 - 663.
AMA koç i,ONAY A,ÖZDEN ÇİFTÇİ Y In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turkish Journal of Biology. 2014; 38(5): 653 - 663.
Vancouver koç i,ONAY A,ÖZDEN ÇİFTÇİ Y In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turkish Journal of Biology. 2014; 38(5): 653 - 663.
IEEE koç i,ONAY A,ÖZDEN ÇİFTÇİ Y "In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)." Turkish Journal of Biology, 38, ss.653 - 663, 2014.
ISNAD koç, ibrahim vd. "In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.)". Turkish Journal of Biology 38/5 (2014), 653-663.
Sayfa Oluşturulma Tarihi
22-04-2024 20:23

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları