Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels
Yıl: 2015 Cilt: 21 Sayı: 5 Sayfa Aralığı: 729 - 737 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels
Öz: Bu çalışmada erkek broiler rasyonlarında klinoptilolitin tek başına veya fitaz ile birlikte kullanılmasının performans, karkas karakteristikleri,bağırsak histomorfoloj isi ve tibia külü ile Ca, P düzeyleri üzerine etkisi incelenmiştir. Toplamda 192 adet günlük yaşta erkek civciv (Ross308) 4 deneme grubuna ve her biri 6 civcivden oluşan 8 tekerrür grubuna rasgele olacak şekilde ayrılmıştır. Birinci grup (T1) kontrol grubuolarak düzenlenerek bazal rasyon ile beslenmişlerdir. İkinci gruba (T2) %2 düzeyinde klinoptilolit ilave edilmiştir. Daha düşük düzeyde Ca veyararlanılabilir P içeren (başlangıç, büyütme ve bitirme dönemleri için sırasıyla Ca; %0.87, 0.80 ve 0.75 yararlanılabilir P; %0.30, 0.28 ve 0.26) 3.ve 4. gruba ise sırasıyla %0.01 fitaz ve %2 klinoptilolit + %0.01 fitaz ilave edilmiştir. Araştırmanın 1-14. günlerde, klinoptilolit ve fitazın birliktekullanıldığı grupta (T4) canlı ağırlık artışının daha düşük düşük olduğu (P=0.044) görülmüştür. Ancak tüm deneme boyunca gruplar arasındacanlı ağırlık artışı, yem tüketimi ve yemden yararlanma oranı açısından farklılık tespit edilmemiştir. Araştırmanın 21. gününde yalnızca fitazilavesinin (T3) duodenum kript derinliğini önemli ölçüde azalttığı (P=0.006) sonucuna varılmıştır. Denemenin 21. gününde tibia külü ve fosfordüzeyinin klinoptilolit ve fitazın birlikte kullanılmasına bağlı olarak önemli ölçüde daha azaldığı görülmüştür. Ancak denemenin 42 günündetibia parametreleri açısından gruplar arasında farklılık tespit edilememiştir. Sonuç olarak başlangıç döneminden sonra rasyonlarda kullanılacak%2 klinoptilolitin performans üzerine olumlu etkileri olabileceği düşünülmektedir.
Anahtar Kelime: Konular:
Klinoptilolit ve/veya Fitaz İlavesinin Broilerlerin Büyüme Performansı, Karkas Karakteristikleri, Bağırsak Histomorfolojisi ve Tibia Kalsiyum ve Fosfor Düzeylerine Olan Etkisi
Öz: The effect of clinoptilolite alone or in combination with phytase on the performance, carcass characteristics, intestinal histomorphology,and tibia ash and Ca, P levels were determined in male broiler chickens. Total of 192 one-day-old male broiler chicks (Ross 308) wererandomly assigned to 4 different treatments with 8 replicates containing 6 birds each. Treatment 1 (T 1) was served as control fed a dietwithout clinoptilolite and/or phytase, Treatment 2 (T2) was supplemented with 2% clinoptilolite. Treatment 3 (T3) and Treatment 4 (T4) were(formulated Ca; 0.87, 0.80 and 0.75%, available P; 0.30, 0.28 and 0.26% for starter, grower, and finisher periods respectively) supplemented0.01% phytase and 2% clinoptilolite + 0.01% phytase, respectively. The results showed that combined use of clinoptilolite and phytase (T4)in broiler diets decreased (P=0.044) body weight gain at 1-14 d. Dietary treatments had no effect on body weight gain, feed intake (FI) andfeed conversion ratio (FCR) during the entire experimental period. Supplementation with phytase (T3) significantly (P=0.006) decreasedcrypt depth in duodenum on d 21. Tibia ash and phosphorus level were decreased due to combined use of clinoptilolite and phytase (T4)in comparison to those fed control (T 1) and clinoptilolite (T2) diets on d 21. At 42 days of age, tibia ash, Ca, P levels and Ca/P ratio was notchanged by the treatments. It is concluded that there might be a positive effect of 2% clinoptilolite on the performance of male broilersafter starting period.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Mumpton FA, Fishman PH: The application of natural zeolites in animal science and aquaculture. J Anim Sci, 45, 1188-1203, 1977.
- 2. Suchy P, Strakova E, Vecerek V, Klouda Z, Kracmarova E: The effect of a clinoptilolite-based feed supplement on the performance of broiler chickens. Czech J Anim Sci, 51, 168-173, 2006.
- 3. Melenova L, Ciahotny K, Jirglova H, Kusa H, Ruzek P: Removal of ammonia from waste gas by means of adsorption on zeolites and their subsequent use in agriculture (in Czech). Chem Listy, 97, 562-568, 2003.
- 4. Wu QJ, Zhou YM, Wu YA, Zhang LL, Wang T: The effects of natural and modified clinoptilolite on intestinal barrier function and immune response to LPS in broiler chickens. Vet Immunol Immunop, 153, 70-76, 2013. DOI: 10.1016/j.vetimm.2013.02.006
- 5. Ortatatli M, Oguz H: Ameliorative effects of dietary clinoptilolite on pathological changes in broiler chickens during aflatoxicosis. Res Vet Sci, 71, 59-66, 2001. DOI: 10.1053/rvsc.2001.0487
- 6. Nakaue HS, Koelliker JK, Pierson ML: Studies with clinoptilolite in poultry. II. Effect of feeding broilers and the direct application of clinoptilolite (zeolite) on clean and reused broiler litter on broiler performance and house environment. Poult Sci, 60, 1221-1228, 1981. DOI: 10.3382/ps.0601221
- 7. Nikolakakis I, Dotas V, Kargopoulos A, Hatzizisis L, Dotas D, Ampas Z: Effect of natural zeolite (clinoptilolite) on the performance and litter quality of broiler chickens. Turk J Vet Anim Sci, 37, 682-686, 2013. DOI: 10.3906/vet-1212-9
- 8. Erdogan BC, Ulku S: Removal of bacteria by clinoptilolite rich mineral and its surfactant modified forms. J Porous Mater, 20, 1143-1151, 2013. DOI: 10.1007/s10934-013-9697-z
- 9. Pappas AC, Tsiplakou E, Georgiadou M, Anagnostopoulos C. Markoglou AN, Liapis K, Zervas G: Bentonite binders in the presence of mycotoxins: Results of in vitro preliminary tests and an in vivo broiler trial. Appl Clay Sci, 99, 48-53, 2014. DOI: 10.1016/j.clay.2014.06.009
- 10. Evans M, Farrell DJ: Are there economic benefits to adding zeolites to poultry diets?, In, Farrell DJ (Ed): Recent Advances in Animal Nutrition in Australia, 303-316, Armidale, University of New England, 1993.
- 11. Öztürk E, Erenler G, Sarıca M: Influence of natural zeolite on performance of laying hens and egg quality. Turk J Agric For, 22, 623-628, 1998.
- 12. Roland DA Sr, Laurent SM, Orloff HD: Shell quality as influenced by zeolite with high ion-exchange capability. Poult Sci, 64, 1177-1187, 1985. DOI: 10.3382/ps.0641177
- 13. Watkins KL, Southern LL: Effect of dietary sodium zeolite A and graded levels of calcium on growth, plasma, and tibia characteristics of chicks. Poult Sci, 70, 2295-2303, 1991. DOI: 10.3382/ps.0702295
- 14. Roland DA Sr, Rabon HW, Rao KS, Smith RC, Miller JW, Barnes DG, Laurent SM: Evidence for absorption of silicon and aluminum by hens fed sodium zeolite. Poult Sci, 72, 447-455, 1993. DOI: 10.3382/ps.0720447
- 15. Utlu N, Celebi S, Yucel O: T he effects of natural zeolite supplementation to diet on serum element concentrations in laying hens. Rev Med Vet, 158, 598-602, 2007.
- 16.Elliot MA, Edwards HM Jr: Some effects of dietary aluminum and silicon in broiler chickens. Poult Sci, 70, 1390-1402, 1991. DOI: 10.3382/ ps.0701390
- 17. Leach RM Jr, Hemrichs BS, Burdette J: Broiler chicks fed low calcium diets. 1. Influence of zeolite on growth rate and parameters of bone metabolism. Poult Sci, 69, 1539-1543, 1990. DOI: 10.3382/ps.0691539
- 18. Ballard R, Edwards HM Jr: Effects of dietary zeolite and vitamin A on tibial dyschondroplasia in chickens. Poult Sci, 67, 113-119, 1988. DOI: 10.3382/ps.0670113
- 19. Scheideler SE: Effect of various types of aluminosilicates and aflatoxin on aflatoxin toxicity, bird performance and mineral status. Poult Sci, 72, 282-288, 1993. DOI: 10.3382/ps.0720282
- 20. Papaioannou D, Katsoulos PD, Panousis N, Karatzias H: The role of natural and synthetic zeolites as feed additives on the prevention and/or the treatment of certain farm animal diseases: a review. Micropor Mesopor Mat, 84, 161-170, 2005. DOI: 10.1016/j.micromeso.2005.05.030
- 21. Watkins KL, Vagnoni DB, Southern LL: Effect of dietary sodium zeolite A and excess calcium on growth and tibia calcium and phosphorus concentration in uninfected and Eimeria acervulina infected chicks. Poult Sci, 68, 1236-1240, 1989. DOI: 10.3382/ps.0681236
- 22. Ravindran V, Bryden WL, Kornegay ET: Phytin: Occurrence, bioavailability and implications in poultry nutrition. Poult Avian Biol Rev, 6, 125-143, 1995.
- 23. Selle PH, Ravindran V: Microbial phytase in poultry nutrition. Anim Feed Sci Technol, 135, 1-41, 2007. DOI: 10.1016/j.anifeedsci.2006.06.010
- 24. Shang Y, Rogiewicz A, Patterson R, Slominski BA, Kim WK: The effect of phytase and fructooligosaccharide supplementation on growth performance, bone quality, and phosphorus utilization in broiler chickens. Poult Sci, 00, 1-10, 2015. DOI: 10.3382/ps/pev044
- 25. El-Sherbiny AE, Hassan HMA, Abd-Elsamee MO, Samy A, Mohamed MA: Performance, bone parameters and phosphorus excretion of broilers fed low phosphorus diets supplemented with phytase from 23 to 40 days of age. Int J Poult Sci, 9, 972-977, 2010. DOI: 10.3923/ij ps.2010.972.977
- 26. Bedford MR: Exogenous enzymes in monogastric nutrition: Their current value and future benefits. Anim Feed Sci Technol, 86, 1-13, 2000. DOI: 10.1016/S0377-8401(00)00155-3
- 27. Selle PH, Ravindran V, Caldwell RA, Bryden WL: Phytate and phytase: Consequences for protein utilization. Nutr Res Rev, 13, 255-278, 2000. DOI: 10.1079/095442200108729098
- 28. Chungc TK, Rutherfurda SM, Thomasb DV, Moughan PJ: Effect of two microbial phytases on mineral availability and retention and bone mineral density in low-phosphorus diets for broilers. Br Poult Sci, 54, 362- 373, 2012. DOI: 10.1080/00071668.2013.783902
- 29. Lalpanmawia H, Elangovan AV, Sridhar M. Shet D, Ajith S, Pal DT: Efficacy of phytase on growth performance, nutrient utilization and bone mineralization in broiler chicken. Anim Feed Sci Technol, 192, 81-89, 2014. DOI: 10.1016/j.anifeedsci.2014.03.004
- 30. National Research Council: Nutrient Requirements for Poultry. 9th rev. ed., National Academy Press, Washington, DC. 1994.
- 31. Culling CFA, Allison RT, Bar WT: Cellular Pathology Technique, 4th ed., 167-171, Butterworth and Co. Ltd., London, 1985.
- 32. Xu ZR, Hu CH, Xia MS, Zhan XA, Wang MQ: Effects of dietary fructo- oligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult Sci, 82, 648-654. 2003. DOI: 10.1093/ ps/82.6.1030
- 33. AOAC: Official Methods of Analysis of the Association of Official Analytical Chemists. 17th ed., Virginia, USA, Method No: 942.05 (ash), 2000.
- 34. Farese G, Schmidt JL, Mager M: An automated method for the determination of serum calcium with glyoxal bis (2-hydroxyanil). Clinical Chem, 13, 515-520, 1967.
- 35. Gericke S, Kurmies B: Die kolorimetrische Phosphorsäurebestimmung mit Ammonium-Vanadat-Moly bdat und ihre Anwendung in der pflanzenanalyse. Z Pflanzenernaehr Dueng Bodenkd, 59, 235-247, 1952.
- 36. Hinkle DE, Wiersma W, Jurs SG: Applied Statistics for the Behavioral Sciences. 5th ed., Houghton Mifflin Company, Boston, MA, USA, 2003.
- 37. Eser H, Yalçın S, Yalçın S, Şehu A: Effects of sepiolite usage in broiler diets on performance, carcass traits and some blood parameters. Kafkas Univ Vet Fak Derg, 18, 313-318, 2012. DOI: 10.9775/kvfd.2011.5472
- 38. Karamanlis X, Fortomaris P, Arsenos G, Dosis I, Papaioannou D, Batzios C, Kamarianos A: The effect of a natural zeolite (clinoptilolite on the performance of broiler chickens and the quality of their litter. Asian-A ust J Anim Sci, 21, 1642-1650, 2008. DOI: 10.5713/ ajas.2008.70652
- 39. Horniakova E, Busta L: Utilization of bio additives in poultry nutrition. Proceedings 43rd Croatian and 3rd International Symposium on Agriculture, Opatija, Croatia, 788-790, 2008.
- 40. Wu QJ, Wang LC, Zhou YM, Zhang JF, Wang T: Effects of clinoptilolite and modified clinoptilolite on growth performance, intestinal microflora and gut parameters of broilers. Poult Sci, 92, 684-692, 2013. DOI: 10.3382/ ps.2012-02308
- 41. Tang ZG, Wen C, Wang LC, Wang T, Zhou YM: Effects of zinc-bearing clinoptilolite on growth performance, cecal microflora and intestinal mucosal function of broiler chickens. Anim Feed Sci Technol, 189, 98-106, 2014. DOI: 10.1016/j.anifeedsci.2013.12.014
- 42. Ramos AJ, Hernandez E: Prevention of aflatoxicosis in farm animals by means of hydrated sodium calcium aluminosilicate addition to feedstuffs: A review. Anim Feed Sci Technol, 65, 197-206, 1997. DOI: 10.1016/S0377-8401(96)01084-X
- 43. Miazzo R, Rosa CA, De Queiroz Carvalho EC, Magnoli C, Chiacchiera SM, Palacio G, Saenz M, Kikot A, Basaldella E, Dalcero A: Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poult Sci, 79, 1-6, 2000. DOI: 10.1093/ps/79.1.1
- 44. Tang ZG, Wen C, Li P, Wang T, Zhou YM: Effect of zinc-bearing zeolite clinoptilolite on growth performance, nutrient retention, digestive enzyme activities, and intestinal function of broiler chickens. Biol Trace Elem Res, 158, 51-57, 2014. DOI: 10.1007/s12011-014-9900-3
- 45. Xia MS, Hu CH, Xu ZR: Effects of copper-bearing montmorillonite on growth performance, digestive enzyme activities, and intestinal microflora and morphology of male broilers. Poult Sci, 83, 1868-1875, 2004. DOI: 10.1093/ps/83.11.1868
- 46. Albengres E, Urien S, Tillement JP, Oury P, Decourt S, Flouvat B, Drieu K: Interactions between smectite, a mucus stabilizer, and acidic and basic drugs. Eur J Clin Pharmacol, 28, 601-605, 1985. DOI: 10.1007/ BF00544074
- 47. Girardeau JP: Smectite aggregation by Escherichia coli. Acta Gastro- Ent Belg, 50, 181-192, 1987.
- 48. Gezen SS, Eren M, Deniz G: The effect of zeolite on broiler performance. Int Vet J, 81, 411-415, 2004.
| APA | SAÇAKLI P, Calik A, BAYRAKTAROGLU A, ERGÜN A, Şahan Yapıcıer Ö, ÖZAYDIN S (2015). Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. , 729 - 737. |
| Chicago | SAÇAKLI PINAR,Calik Ali,BAYRAKTAROGLU ALEV GÜROL,ERGÜN Ahmet,Şahan Yapıcıer Özlem,ÖZAYDIN Serpil Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. (2015): 729 - 737. |
| MLA | SAÇAKLI PINAR,Calik Ali,BAYRAKTAROGLU ALEV GÜROL,ERGÜN Ahmet,Şahan Yapıcıer Özlem,ÖZAYDIN Serpil Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. , 2015, ss.729 - 737. |
| AMA | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. . 2015; 729 - 737. |
| Vancouver | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. . 2015; 729 - 737. |
| IEEE | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S "Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels." , ss.729 - 737, 2015. |
| ISNAD | SAÇAKLI, PINAR vd. "Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels". (2015), 729-737. |
| APA | SAÇAKLI P, Calik A, BAYRAKTAROGLU A, ERGÜN A, Şahan Yapıcıer Ö, ÖZAYDIN S (2015). Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(5), 729 - 737. |
| Chicago | SAÇAKLI PINAR,Calik Ali,BAYRAKTAROGLU ALEV GÜROL,ERGÜN Ahmet,Şahan Yapıcıer Özlem,ÖZAYDIN Serpil Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.5 (2015): 729 - 737. |
| MLA | SAÇAKLI PINAR,Calik Ali,BAYRAKTAROGLU ALEV GÜROL,ERGÜN Ahmet,Şahan Yapıcıer Özlem,ÖZAYDIN Serpil Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.5, 2015, ss.729 - 737. |
| AMA | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 729 - 737. |
| Vancouver | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 729 - 737. |
| IEEE | SAÇAKLI P,Calik A,BAYRAKTAROGLU A,ERGÜN A,Şahan Yapıcıer Ö,ÖZAYDIN S "Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.729 - 737, 2015. |
| ISNAD | SAÇAKLI, PINAR vd. "Effect of Clinoptilolite and/or Phytase on Broiler Growth Performance, Carcass Characteristics, Intestinal Histomorphology and Tibia Calcium and Phosphorus Levels". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/5 (2015), 729-737. |
