Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

KAYGUSUZ, İrfan; Orhan, Israfil; karlıdag, turgut; ŞİMŞEK ÇOBANOĞLU, Bengü; sapmaz, emrah; Keleş, Erol; YALÇIN, Şinasi; IŞIK, Özgür

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Özgür IŞIK, (Fırat Üniversitesi, Eğitim ve Araştırma Hastanesi, Elazığ, Türkiye)

Turgut KARLIDAĞ, (Fırat Üniversitesi, Eğitim ve Araştırma Hastanesi, Elazığ, Türkiye)

Bengü ŞİMŞEK ÇOBANOĞLU, (Fırat Üniversitesi, Fırat Tıp Merkezi, Patoloji Anabilim Dalı, Elazığ, Türkiye)

Erol KELEŞ, (Fırat Üniversitesi, Eğitim ve Araştırma Hastanesi, Elazığ, Türkiye)

İrfan KAYGUSUZ, (Fırat Üniversitesi, Eğitim ve Araştırma Hastanesi, Elazığ, Türkiye)

Şinasi YALÇIN, (Fırat Üniversitesi, Eğitim ve Araştırma Hastanesi, Elazığ, Türkiye)

İsrafil ORHAN, (Kahramanmaraş Sütçü İmam Üniversitesi, Kahramanmaraş, Türkiye)

Emrah SAPMAZ (Gaziosmanpaşa Üniversitesi, Araştırma ve Uygulama Hastanesi, Tokat, Türkiye)

Türk Otorinolarengoloji Arşivi

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Yıl: 2015 Cilt: 53 Sayı: 2 Sayfa Aralığı: 55 - 61 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma

Öz:

0, no staining; + cell staining (weak positive staining:Amaç: Bu çalışmanın amacı primer ve sekonder akkizkolesteatomlardaki bcl-2, bax ve c-erbB-2 ekspresyon-larını değerlendirmek ve kolesteatom patogenezindeki hücre proliferasyonu ve apapitozisin rolünü belirle-mektir.Yöntemler: Kolesteatomsuz kronik otitis media (KOM)nedeniyle opere edilen hastaların dış kulak yolu cildin-den (DKY) alınan örnekler grup 1; kolesteatomlu KOMnedeniyle opere edilen grup 3 ve grup 4deki hastalarınDKY cildinden alınan örnekler grup 2; primer akkiz ko-lesteatomlu KOM nedeniyle opere edilen hastaların ko-lesteatom matriksinden alınan örnekler grup 3; sekonder akkiz kolesteatomlu KOM nedeniyle opere edilen has-taların kolesteatom matriksinden alınan örnekler grup4ü oluşturdu. Pozitif hücrelerin oranının değerlendir-mesi belirtilen bulguların varlığı bazında yapıldı ve se-mikantitatif olarak dört grupta sınıflandı: 0, boyama yok;+ hücre boyanması (hafif derecede boyanma: %l-33); ++hücre boyanması (orta derecede boyanma: %34-66); +++1% 33%); ++ cell staining (moderately positive staining:34% 66%); and +++ cell staining (strong positive stain-ing: 67% 100%).Results: Comparison of the staining scores of bcl-2, bax, and c-erbB-2 revealed a statistically insignificant differ-ence in the staining of samples obtained from the EECskin (p>0.05). Decreased bcl-2 expression and increasedbax and c-erbB-2 expressions were determined in pri-mary and secondary acquired cholesteatoma epitheliumcompared with the EEC skin of patients operated forCOM with or without cholesteatoma, and the differ-ences were found to be statistically significant (p<0.05).Conclusion: In acquired cholesteatoma epithelium, thefinding of decreased bcl-2 expression as well as increasedbax and c-erbB-2 expressions compared with the EECskin is an indicator of the increase in both cellular pro-liferation and apoptosis.

Anahtar Kelime:

Konular: Biyoloji

Kolesteatomlu ve Kolesteatomsuz Kronik Otitli Hastalarda bcl-2, bax ve c-erbB-2 Düzeylerinin Değerlendirilmesi

Öz:

Objective: The aim of this study was to evaluate bcl-2,bax, and c-erbB-2 expressions in primary and secondaryacquired cholesteatoma and to indicate the role of apop-tosis and accompanying increased cellular proliferation in the pathogenesis of cholesteatoma.Methods: Samples obtained from the skin of the ex-ternal ear canal (EEC) of patients operated for chronicotitis media (COM) without cholesteatoma constitut-ed Group 1; samples from the EEC skin of patients inGroup 3 operated for COM with cholesteatoma andfrom the EEC skin of patients in Group 4 constitut-ed Group 2; samples obtained from the cholesteatomamatrix of patients operated for COM with primary ac- quired cholesteatoma constituted Group 3; and samplesobtained from the cholesteatoma matrix of patients op-erated for COM with secondary acquired cholesteatomaconstituted Group 4. The assessment of the positive cellratio was based on the presence of the following findingsand was semiquantitatively classified into four groups:0, no staining; + cell staining (weak positive staining:

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	IŞIK Ö, karlıdag t, ŞİMŞEK ÇOBANOĞLU B, Keleş E, KAYGUSUZ İ, YALÇIN Ş, Orhan I, sapmaz e (2015). Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. , 55 - 61.
Chicago	IŞIK Özgür,karlıdag turgut,ŞİMŞEK ÇOBANOĞLU Bengü,Keleş Erol,KAYGUSUZ İrfan,YALÇIN Şinasi,Orhan Israfil,sapmaz emrah Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. (2015): 55 - 61.
MLA	IŞIK Özgür,karlıdag turgut,ŞİMŞEK ÇOBANOĞLU Bengü,Keleş Erol,KAYGUSUZ İrfan,YALÇIN Şinasi,Orhan Israfil,sapmaz emrah Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. , 2015, ss.55 - 61.
AMA	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. . 2015; 55 - 61.
Vancouver	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. . 2015; 55 - 61.
IEEE	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e "Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma." , ss.55 - 61, 2015.
ISNAD	IŞIK, Özgür vd. "Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma". (2015), 55-61.

APA	IŞIK Ö, karlıdag t, ŞİMŞEK ÇOBANOĞLU B, Keleş E, KAYGUSUZ İ, YALÇIN Ş, Orhan I, sapmaz e (2015). Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. Türk Otorinolarengoloji Arşivi, 53(2), 55 - 61.
Chicago	IŞIK Özgür,karlıdag turgut,ŞİMŞEK ÇOBANOĞLU Bengü,Keleş Erol,KAYGUSUZ İrfan,YALÇIN Şinasi,Orhan Israfil,sapmaz emrah Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. Türk Otorinolarengoloji Arşivi 53, no.2 (2015): 55 - 61.
MLA	IŞIK Özgür,karlıdag turgut,ŞİMŞEK ÇOBANOĞLU Bengü,Keleş Erol,KAYGUSUZ İrfan,YALÇIN Şinasi,Orhan Israfil,sapmaz emrah Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. Türk Otorinolarengoloji Arşivi, vol.53, no.2, 2015, ss.55 - 61.
AMA	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. Türk Otorinolarengoloji Arşivi. 2015; 53(2): 55 - 61.
Vancouver	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma. Türk Otorinolarengoloji Arşivi. 2015; 53(2): 55 - 61.
IEEE	IŞIK Ö,karlıdag t,ŞİMŞEK ÇOBANOĞLU B,Keleş E,KAYGUSUZ İ,YALÇIN Ş,Orhan I,sapmaz e "Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma." Türk Otorinolarengoloji Arşivi, 53, ss.55 - 61, 2015.
ISNAD	IŞIK, Özgür vd. "Evaluation of bcl-2, bax and c-erbB-2 Levels in Chronic Otitis Patients with or without Cholesteatoma". Türk Otorinolarengoloji Arşivi 53/2 (2015), 55-61.