The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers

KOÇDEMİR, Esra; MİLLETLİ SEZGİN, FİKRİYE; demir, tulin

The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers

Tülin DEMİR, (Ahi Evran Üniversitesi, Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Anabilim Dalı, Kırşehir, Türkiye)

Esra KOÇDEMİR, (Ahi Evran Üniversitesi, Eğitim ve Araştırma Hastanesi, Biyokimya Anabilim Dalı, Kırşehir, Türkiye)

FİKRİYE MİLLETLİ SEZGİN (Ahi Evran Üniversitesi, Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Anabilim Dalı, Kırşehir, Türkiye)

Viral Hepatitis Journal

2 1

Yıl: 2014 Cilt: 20 Sayı: 1 Sayfa Aralığı: 4 - 7 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers

Öz:

Amaç: Bu çalışmada hepatit B virüsü (HBV)-DNA düzeyleri ile biyokimyasalparametreler, yaş, cinsiyet, HBV serolojik göstergeleri arasındaki ilişki incelenmiştir. Gereç ve Yöntemler: Çalışmaya, kronik hepatit B hastalarından alınan HBsAg(+) 124 serum örneği dâhil edilmiştir. Örneklerde HBV-DNA düzeyi, HBV serolojisi,alanin transaminaz (ALT), aspartat transaminaz, gama-glutamil transferaz (GGT),lipaz, bilirubin, laktat dehidrogenaz, C-reaktif protein düzeyleri incelenmiştir. HBV- DNA düzeyi gerçek zamanlı polimeraz zincir reaksiyonu (PCR) ile serolojik belirteçlerise ELISA ile değerlendirilmiştir. Bulgular: Moleküler inceleme sonucunda 116 örnekte HBV-DNA varlığı tespitedilmiş, bunların %36,2sinin HBV-DNA düzeyinin 2000 IU/mLnin üzerinde olduğubelirlenmiştir. HBV-DNA düzeyi 2000 IU/mLnin üzerinde olan örnekler kadınlardadaha yüksek sıklıkta bulunmuştur. ALT düzeyinde artış saptanan HBeAg (+)hastaların %44,4ünde HBV-DNA düzeyi 2000 IU/mLnin üzerinde belirlenmiştir. ALTyüksekliği HBeAg (+) tüm hastalarda, HBeAg (-) olanların %24,4ünde ve anti-HBe(+) hastaların %25,5inde izlenmiştir. Yirmi yedisinde ALT artışı belirlenen 106 anti- HBe (+) hastasının 99unda PCR pozitif olarak saptanmıştır. HBeAg pozitifliği izlenentüm örneklerde HBV-DNA (+) olarak belirlenmiştir. GGT dışındaki tüm biyokimyasalparametrelerde yükseklik, HBV-DNA düzeyi 2000 IU/mLnin üzerinde olan hastalarda2000 IU/mL altındaki hastalara göre daha yüksek sıklıkta bulunmuştur. İstatistikselolarak anlamlı bir ilişki sadece lipaz ile HBV-DNA düzeyi arasında belirlenmiştir.Sonuç: Enfektivitenin tespitinde HBeAg serokonversiyonu tek başına yeterlideğildir, yanı sıra HBV serolojik testleri, HBV-DNA düzeyleri ve transaminazseviyeleri ile birlikte hastanın klinik durumunun da tanıda değerlendirilmesigerekmektedir.

Anahtar Kelime:

Konular: Gastroenteroloji ve Hepatoloji

Hepatit B Virüs (HBV)-DNA Seviyeleri ve Biyokimyasal Belirteçler Arasındaki İlişki

Öz:

Objective: In the present study, we aimed to evaluate the association betweenHBV-DNA levels and biochemical parameters, age, gender, and hepatitis B virus(HBV) serologic markers. Materials and Methods: A total of 124 HBsAg (+) serum samples of the patientswith chronic hepatitis B, were included in the study. HBV-DNA level, HBV serologicalmarkers, alanine transaminase (ALT), aspartat transaminase, gama-glutamyl trans- ferase (GGT), lipase, bilirubin, lactate dehydrogenase, and C-reactive protein levelsin the samples were evaluated. HBV-DNA levels were quantitatively evaluated byreal-time polymerase chain reaction (PCR) and serological markers were evaluatedby ELISA. Results: With molecular testing, 116 samples were positive for HBV-DNA, ofthem 36.2% had an HBV-DNA level >2000 IU/mL. The number of samples withHBV-DNA levels >2000 IU/mL were higher in females than those of males. 44.4%of all HBeAg (+) patients with elevated ALT levels had an HBV-DNA level >2000 IU/ mL. All HBeAg (+) patients, 24.4% of HBeAg (-) patients, and 25.5% of anti-HBe (+)patients had elevated ALT levels. 99 of the 106 anti-HBe (+) carriers, of whom 27had elevated ALT levels, were PCR positive. All HBeAg-positive samples were HBV- DNA positive. The rate of samples with elevated biochemical parameters exceptGGT levels were higher serian patients with HBV-DNA level >2000 IU/mL than inpatients with HBV-DNA level <2000 IU/mL. A statistically significant relationshipwas detected only between lipase and HBV-DNA levels. Conclusion: It is clear that HBeAg seroconversion is not sufficiently definitive todetermine the infectivity and it is crucial to evaluate HBV serological tests, HBV- DNA levels, transaminases levels besides the clinical picture of the patient in thediagnosis of the infection.

Anahtar Kelime:

Konular: Gastroenteroloji ve Hepatoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. sharma Ks, saini n, chwla Y. Hepatitis B Virus: Inactive carriers. Virol J. 2005; 2: 82.
2. Lok AsF, McMahon BJ. AAsLD Practice Guidelines. chronic Hepatitis B. Hepatology. 2007; 45(2): 507-539.
3. Lai cL, ratziu V, Yuen MF, Poynard T. Viral hepatitis B. Lancet. 2003; 362: 2089-2094.
4. World Health Organization, Department of communicable Diseases and surveillance and response. 2002. Hepatitis B. online. http://www.who.int/csr/disease/hepatitis/HepatitisB_ whocdscsrlyo2002_2.pdf.
5. custer B, sullivan sD, Hazlet TK, Iloeje u, Veenstra DL, Kowdley KV. Global epidemiology of Hepatitis B virus. J clin Gastroenterol. 2004; 38(10 suppl 3): 158-168.
6. chang MH. Hepatitis B virus infection. semin Fetal neonatal Med. 2007; 12: 160-167.
7. Weinbaum cM1, Williams I, Mast ee, Wang sA, Finelli L, Wasley A; centers for Disease control and Prevention (cDc). recommendations for Identification and Public Health Management of Persons with chronic Hepatitis B Virus Infection. MMWr recomm rep. 2008; 57(rr-8): 1-20.
8. Liaw YF, Brunetto Mr, Hadziyannis s. The natural history of chronic HBV infection and geographical differences. Antivir Ther. 2010; 15 suppl 3:25-33.
9. robinson Ws. Hepatitis B virus and hepatitis D virus. In: Mandell GL, Bennett Je, Dolin r, eds. Principles and Practice of Infectious Diseases, 4th ed. new York, churchill Livingstone, 1995:1406-39.
10. european Association For The study Of The Liver. eAsL clinical practice guidelines: Management of chronic hepatitis B virus infection. european Association For The study Of The Liver. J Hepatol. 2012; 57: 167-85.
11. chu cM, Liaw YF. Hepatitis B surface antigen seroclearance during chronic HBV infection. Antivir Ther. 2010; 15: 133143.
12. Amarapurkar D. Management of hepatitis B viral infection with normal ALT. Hep B Annual. 2006; 3: 155-164.
13. curry MP, chopra s. Acute Viral Hepatitis. In:Mandell GL, Bennett Je, Dolin r, (eds). Principles and Practice of Infectious Diseases. 7th ed.Philadelphia: churchill Livingstone; 2010. 1577-1592.
14. coopstead, Lee-ellen c. 2010; Pathophysiology. Missouri: saunders. pp. 886-887.
15. Alter MJ. epidemiology of hepatitis B in europe and worldwide. J Hepatol. 2003; 39(1): 64-69.
16. chu cM, Liaw YF. Predictive factors for reactivation of hepatitis B following hepatitis B e antigen seroconversion in chronic hepatitis B. Gastroenterology. 2007; 133: 1458-1465.
17. Lok As, Heathcote eJ, Hoofnagle JH. review Management of hepatitis B: 2000--summary of a workshop. Gastroenterology. 2001; 120: 1828-1853.
18. Lok As, McMahon BJ. chronic hepatitis B. Hepatology. 2001; 34: 1225-1241.
19. Lok AsF, Lai cL, Wu Pc, Leung eKY, Lam Ts. spontaneous hepatitis B e antigen to antibody seroconversion and reversion in chinese patients with chronic hepatitis B virus infection. Gastroenterology. 1987; 92: 1839-1843.
20. Wang JT, Wang TH, sheu Jc, shih Ln, Lin JT, chen Ds. Detection of hepatitis B virus DnA by polymerase chain reaction in plasma of volunteer blood donors negative for hepatitis B surface antigen. J Infect Dis. 1991; 163: 397-379.
21. Tsai JF1, chuang LY, Jeng Je, Ho Ms, Lin zY, Hsieh MY, et al. sex differences in relation to serum hepatitis B e antigen and alanine aminotransferase levels among asyptomatic hepatitis B surface antigen carriers. J Gastroenterol. 2000; 35: 690-695.
22. chu cM, sheen Is, Lin sM, Liaw YF. sex differance in chronic hepatitis B virüs infection: studies of serum HBeAg and alanine aminotransferase levels in 10,431 asymptomatic chinese HBsAg carriers. clin Infect Dis. 1993; 16: 709-713.
23. Hui AY, chan HLY, Wong VWs, Liew cT, chim AML, chan FKL, et al. Identification of chronic hepatitis B patients without significant liver fibrosis by a simple noninvasive predictive model. Am J Gastroenterol. 2005; 100: 616-623.

APA	demir t, KOÇDEMİR E, MİLLETLİ SEZGİN F (2014). The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. , 4 - 7.
Chicago	demir tulin,KOÇDEMİR Esra,MİLLETLİ SEZGİN FİKRİYE The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. (2014): 4 - 7.
MLA	demir tulin,KOÇDEMİR Esra,MİLLETLİ SEZGİN FİKRİYE The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. , 2014, ss.4 - 7.
AMA	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. . 2014; 4 - 7.
Vancouver	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. . 2014; 4 - 7.
IEEE	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F "The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers." , ss.4 - 7, 2014.
ISNAD	demir, tulin vd. "The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers". (2014), 4-7.

APA	demir t, KOÇDEMİR E, MİLLETLİ SEZGİN F (2014). The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. Viral Hepatitis Journal, 20(1), 4 - 7.
Chicago	demir tulin,KOÇDEMİR Esra,MİLLETLİ SEZGİN FİKRİYE The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. Viral Hepatitis Journal 20, no.1 (2014): 4 - 7.
MLA	demir tulin,KOÇDEMİR Esra,MİLLETLİ SEZGİN FİKRİYE The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. Viral Hepatitis Journal, vol.20, no.1, 2014, ss.4 - 7.
AMA	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. Viral Hepatitis Journal. 2014; 20(1): 4 - 7.
Vancouver	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers. Viral Hepatitis Journal. 2014; 20(1): 4 - 7.
IEEE	demir t,KOÇDEMİR E,MİLLETLİ SEZGİN F "The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers." Viral Hepatitis Journal, 20, ss.4 - 7, 2014.
ISNAD	demir, tulin vd. "The Association Between Hepatitis B Virus (HBV)-DNA Levels and Biochemical Markers". Viral Hepatitis Journal 20/1 (2014), 4-7.