The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos

Alper KOÇYİĞİT, (Cumhuriyet Üniversitesi, Veteriner Fakültesi, Sivas, Türkiye)
Mesut ÇEVİK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun'i Tohumlama Anabilim Dalı, Samsun, Türkiye)
Uğur ŞEN, (Ahi Evran Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Kırşehir, Türkiye)
Mehmet KURAN (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Samsun, Türkiye)
Turkish Journal of Veterinary and Animal Sciences
1 0
Yıl: 2015 Cilt: 39 Sayı: 3 Sayfa Aralığı: 308 - 313 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos

Öz:
This study was conducted to determine the effects of diferent macromolecule sources added to synthetic oviduct fuid (SOF)culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes weredistributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm(TE) and inner cell mass (ICM) cells were determined by diferential staining method. It was found that bovine serum albumin (BSA),either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryoyield and faster development during early bovine embryo culture. The quality of bovine embryos, based on the number of blastocyst cellsand the ratio of ICM to total blastomeres, was afected by the sources of macromolecules and their combinations with growth factors.Growth factors supplemented to SOFaa media with BSA and PVA signifcantly increased the number of ICM cells and the ratio of ICMcells to total number of cells. In conclusion, replacing BSA with PVA depressed the blastocyst rate and cell numbers, and the number ofblastomeres and ICM and TE cell numbers were afected by both the type of macromolecule and the growth factor supplements.
Anahtar Kelime:

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KOÇYİĞİT A, CEVIK M, ŞEN U, Kuran M (2015). The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. , 308 - 313.
Chicago KOÇYİĞİT ALPER,CEVIK MESUT,ŞEN Uğur,Kuran Mehmet The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. (2015): 308 - 313.
MLA KOÇYİĞİT ALPER,CEVIK MESUT,ŞEN Uğur,Kuran Mehmet The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. , 2015, ss.308 - 313.
AMA KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. . 2015; 308 - 313.
Vancouver KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. . 2015; 308 - 313.
IEEE KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M "The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos." , ss.308 - 313, 2015.
ISNAD KOÇYİĞİT, ALPER vd. "The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos". (2015), 308-313.
APA KOÇYİĞİT A, CEVIK M, ŞEN U, Kuran M (2015). The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences, 39(3), 308 - 313.
Chicago KOÇYİĞİT ALPER,CEVIK MESUT,ŞEN Uğur,Kuran Mehmet The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences 39, no.3 (2015): 308 - 313.
MLA KOÇYİĞİT ALPER,CEVIK MESUT,ŞEN Uğur,Kuran Mehmet The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences, vol.39, no.3, 2015, ss.308 - 313.
AMA KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences. 2015; 39(3): 308 - 313.
Vancouver KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos. Turkish Journal of Veterinary and Animal Sciences. 2015; 39(3): 308 - 313.
IEEE KOÇYİĞİT A,CEVIK M,ŞEN U,Kuran M "The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos." Turkish Journal of Veterinary and Animal Sciences, 39, ss.308 - 313, 2015.
ISNAD KOÇYİĞİT, ALPER vd. "The effect of macromolecule and growth factor combinations on in vitro development of bovine embryos". Turkish Journal of Veterinary and Animal Sciences 39/3 (2015), 308-313.
Sayfa Oluşturulma Tarihi
22-04-2024 21:7

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