The effects of thalidomide in a rat model of surgically- induced endometriosis

Amaç: Bu çalışmanın amacı, talidomidin anti-anjiyojenik rolünü analiz etmek ve talidomid tedavisinin cerrahi olarak endometriozis oluşturulan bir sıçan modeli üzerinde etkisi bulunup bulunmadığını belirlemektir. Gereç ve Yöntem: Toplam 16 sıçanda cerrahi indüksiyon ve homolog transplantasyon aracılığıyla endometriozis oluşturulmuştur. Sıçanlar rastgele talidomid (n=8) ve kontrol (n=8) olmak üzere iki gruba ayrılmıştır. Oral gavajla talidomid grubuna 0,5 ml talidomid 100 mg/kg, kontrol grubuna ise 0,5 ml salin verilmiştir. Dört hafta sonra histopatolojik bulgular ve implantların hacim analizi değerlendirilmiştir. Vasküler endotelyal büyüme faktörü (VEGF-A) ile oksidatif belirteçler peritoneal lavaj sıvısında çalışılmıştır. Bulgular: Tedaviden sonra implant hacmi talidomid verilen grupta anlamlı olarak azalmıştır (sırasıyla 53,3 ve 22,9 mm3, p=0,012). Talidomid grubunun histopatolojik skorlarında saptanan anlamlı farklar, kontrol grubunda rastlanmamıştır (sırasıyla 3 ve 1, p=0,012). Oksidatif belirteçlerden VEGF-A ve miyeloperoksidaz (MPO) düzeylerinde anlamlı azalmalar gözlemlenmiştir (sırasıyla p=0,004 ve p=0,037). Sonuç: Özellikle VEGF inhibisyonu ve anti-anjiyojenik etkiye bağlı olarak talidomidin implantlarda volümetrik ve histopatolojik iyileşme sağlaması, emdometriozis tedavisinde etkili olabileceğini düşündürmektedir.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Hematoloji

induced endometriosis Cerrahi olarak endometriozis oluşturulan bir sıçan modelinde talidomidin etkileri

Öz:

Abstract Objective: The aim of the study was to analyze the anti-angiogenic role of thalidomide and to assess whether thalidomide had any influence on a rat model of surgically-induced endometriosis. Materials and Methods: Endometriosis was induced through surgical induction and homologous transplantation in 16 rats. The rats were randomly separated into two groups as thalidomide (n=8) and control (n=8) groups. Using oral gavage, 100 mg/kg thalidomide 0.5 ml was administered to the first group and saline 0.5 ml to the control group. Histopathologic findings and volume analysis of implants were evaluated after 4 weeks. Vascular endothelial growth factor-A (VEGF-A) and oxidative markers were run from the fluid through peritoneal lavage. Results: The average implant volume decreased significantly in the thalidomide administrated group after treatment (53.3 and 22.9 mm3 respectively, p=0.012). Significant differences observed in the histopathologic scores of the thalidomide group (3 and 1 respectively, p=0.012) were not observed in the control group. Significant decreases were observed in the levels of VEGF-A and myeloperoxidase (MPO) from oxidative markers (p=0.004, p=0.037, respectively). Conclusion: Thalidomide provides volumetric and histopathologic recovery in implants particularly because the VEGF inhibition and anti-angiogenic effect, which suggests that it could be effective in the treatment of endometriosis. Keywords: Animal model, thalidomide, endometriosis, vascular endothelial growth factor

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Hematoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAKACAK S, ERCAN Ö, KÖSTÜ B, bostanci m, İNANÇ TOLUN F, YAYLALI A, serin s, BALAKAN O, Kiran G (2015). The effects of thalidomide in a rat model of surgically- induced endometriosis. , 125 - 131.
Chicago	BAKACAK SÜLEYMAN MURAT,ERCAN Önder,KÖSTÜ Bülent,bostanci mehmet suhha,İNANÇ TOLUN FATMA,YAYLALI ASLI,serin salih,BALAKAN Ozan,Kiran Gurkan The effects of thalidomide in a rat model of surgically- induced endometriosis. (2015): 125 - 131.
MLA	BAKACAK SÜLEYMAN MURAT,ERCAN Önder,KÖSTÜ Bülent,bostanci mehmet suhha,İNANÇ TOLUN FATMA,YAYLALI ASLI,serin salih,BALAKAN Ozan,Kiran Gurkan The effects of thalidomide in a rat model of surgically- induced endometriosis. , 2015, ss.125 - 131.
AMA	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G The effects of thalidomide in a rat model of surgically- induced endometriosis. . 2015; 125 - 131.
Vancouver	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G The effects of thalidomide in a rat model of surgically- induced endometriosis. . 2015; 125 - 131.
IEEE	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G "The effects of thalidomide in a rat model of surgically- induced endometriosis." , ss.125 - 131, 2015.
ISNAD	BAKACAK, SÜLEYMAN MURAT vd. "The effects of thalidomide in a rat model of surgically- induced endometriosis". (2015), 125-131.

APA	BAKACAK S, ERCAN Ö, KÖSTÜ B, bostanci m, İNANÇ TOLUN F, YAYLALI A, serin s, BALAKAN O, Kiran G (2015). The effects of thalidomide in a rat model of surgically- induced endometriosis. Türk Jinekoloji ve Obstetrik Derneği Dergisi, 12(3), 125 - 131.
Chicago	BAKACAK SÜLEYMAN MURAT,ERCAN Önder,KÖSTÜ Bülent,bostanci mehmet suhha,İNANÇ TOLUN FATMA,YAYLALI ASLI,serin salih,BALAKAN Ozan,Kiran Gurkan The effects of thalidomide in a rat model of surgically- induced endometriosis. Türk Jinekoloji ve Obstetrik Derneği Dergisi 12, no.3 (2015): 125 - 131.
MLA	BAKACAK SÜLEYMAN MURAT,ERCAN Önder,KÖSTÜ Bülent,bostanci mehmet suhha,İNANÇ TOLUN FATMA,YAYLALI ASLI,serin salih,BALAKAN Ozan,Kiran Gurkan The effects of thalidomide in a rat model of surgically- induced endometriosis. Türk Jinekoloji ve Obstetrik Derneği Dergisi, vol.12, no.3, 2015, ss.125 - 131.
AMA	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G The effects of thalidomide in a rat model of surgically- induced endometriosis. Türk Jinekoloji ve Obstetrik Derneği Dergisi. 2015; 12(3): 125 - 131.
Vancouver	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G The effects of thalidomide in a rat model of surgically- induced endometriosis. Türk Jinekoloji ve Obstetrik Derneği Dergisi. 2015; 12(3): 125 - 131.
IEEE	BAKACAK S,ERCAN Ö,KÖSTÜ B,bostanci m,İNANÇ TOLUN F,YAYLALI A,serin s,BALAKAN O,Kiran G "The effects of thalidomide in a rat model of surgically- induced endometriosis." Türk Jinekoloji ve Obstetrik Derneği Dergisi, 12, ss.125 - 131, 2015.
ISNAD	BAKACAK, SÜLEYMAN MURAT vd. "The effects of thalidomide in a rat model of surgically- induced endometriosis". Türk Jinekoloji ve Obstetrik Derneği Dergisi 12/3 (2015), 125-131.