IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase

İMİR, Nilüfer Gülmen; ÖĞÜŞ, Mehmet; ÖZEŞ, Osman Nidai; GELEN, Tekinalp; NASIRCILAR, AYSE GÜL; AKÇA, Hakan; KARAVELİ, Şeyda

IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase

Nilüfer Gülmen İMİR, (Akdeniz Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Anabilim Dalı, Antalya, Türkiye)

Ayşe Gül NASIRCILAR, (Girilmedi)

Hakan AKÇA, (Girilmedi)

Tekinalp GELEN, (Girilmedi)

Mehmet ÖĞÜŞ, (Girilmedi)

Şeyda KARAVELİ, (Girilmedi)

Osman Nidai ÖZEŞ (Girilmedi)

Turkish Journal of Medical Sciences

1 0

Yıl: 2002 Cilt: 32 Sayı: 3 Sayfa Aralığı: 195 - 199 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase

Öz:

We investigated whether the activity of double-stranded RNA activated kinase (p68/PKR) is necessary for induction of IDO (indolamine 2,3 -dioxygenase) gene by IFN-$gamma$. For this purpose, we planned to abrogate the function of cellular wt-PKR gene. Clones of ME180 cells expressing the dominant negative form of PKR were selected in the presence of neomycin. Then these cells were treated with IFN-$gamma$ and accumulation of IDO protein was determined by Western blot analysis. We found that clones expressing the dominant negative form of the PKR gene were not only resistant to IFN-$gamma$-mediated killing, but were also incapable of inducing accumulation of IDO protein after IFN-$gamma$ treatment.

Anahtar Kelime: eIF-2 Kinase Indolamine Interferons Transcription, Genetic Cell Culture

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	İMİR N, NASIRCILAR A, AKÇA H, GELEN T, ÖĞÜŞ M, KARAVELİ Ş, ÖZEŞ O (2002). IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. , 195 - 199.
Chicago	İMİR Nilüfer Gülmen,NASIRCILAR AYSE GÜL,AKÇA Hakan,GELEN Tekinalp,ÖĞÜŞ Mehmet,KARAVELİ Şeyda,ÖZEŞ Osman Nidai IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. (2002): 195 - 199.
MLA	İMİR Nilüfer Gülmen,NASIRCILAR AYSE GÜL,AKÇA Hakan,GELEN Tekinalp,ÖĞÜŞ Mehmet,KARAVELİ Şeyda,ÖZEŞ Osman Nidai IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. , 2002, ss.195 - 199.
AMA	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. . 2002; 195 - 199.
Vancouver	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. . 2002; 195 - 199.
IEEE	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O "IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase." , ss.195 - 199, 2002.
ISNAD	İMİR, Nilüfer Gülmen vd. "IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase". (2002), 195-199.

APA	İMİR N, NASIRCILAR A, AKÇA H, GELEN T, ÖĞÜŞ M, KARAVELİ Ş, ÖZEŞ O (2002). IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. Turkish Journal of Medical Sciences, 32(3), 195 - 199.
Chicago	İMİR Nilüfer Gülmen,NASIRCILAR AYSE GÜL,AKÇA Hakan,GELEN Tekinalp,ÖĞÜŞ Mehmet,KARAVELİ Şeyda,ÖZEŞ Osman Nidai IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. Turkish Journal of Medical Sciences 32, no.3 (2002): 195 - 199.
MLA	İMİR Nilüfer Gülmen,NASIRCILAR AYSE GÜL,AKÇA Hakan,GELEN Tekinalp,ÖĞÜŞ Mehmet,KARAVELİ Şeyda,ÖZEŞ Osman Nidai IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. Turkish Journal of Medical Sciences, vol.32, no.3, 2002, ss.195 - 199.
AMA	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. Turkish Journal of Medical Sciences. 2002; 32(3): 195 - 199.
Vancouver	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase. Turkish Journal of Medical Sciences. 2002; 32(3): 195 - 199.
IEEE	İMİR N,NASIRCILAR A,AKÇA H,GELEN T,ÖĞÜŞ M,KARAVELİ Ş,ÖZEŞ O "IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase." Turkish Journal of Medical Sciences, 32, ss.195 - 199, 2002.
ISNAD	İMİR, Nilüfer Gülmen vd. "IFN-gamma-mediated transcriptional induction of the IDO (Indolamine 2,3-dioxygenase) gene requires activity of p68/PKR protein kinase". Turkish Journal of Medical Sciences 32/3 (2002), 195-199.