Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits
Yıl: 2011 Cilt: 26 Sayı: 1 Sayfa Aralığı: 13 - 18 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits
Öz: Amaç: Bu çalışmada romatoid faktörün (RF), anti-siklik sitrülin peptit (anti-CCP) antikorunun ve anti-mutat sitrülin vimentin (anti-MCV) antikorunun iki farklı ticari kitinin, ticari kitlerin performansları arasında önemli farklılıklar bulunabileceğini de göz önünde bulundurarak, romatoid artritteki (RA) tanısal değerleri test edildi.Hastalar ve yöntemler: Ekim 2008 ile Şubat 2009 tarihleri arasında romatoloji polikliniğimize başvuran 34 RA'lı hasta ve 24 sağlıklı kontrol çalışmaya alındı. Romatoid artritli hastaların ve sağlıklı gönüllülerin serumlarında iki farklı ticari kitle RF, antiCCP-2, anti-MCV-548 ve anti-MCV-248 otoantikorları çalışıldı. Romatoid artritli hastalarda hastalık aktiviteleri, hastalık aktivite skoru 28 (DAS-28)'e göre belirlendi. Romatoid artritli hastalarda eklem dışı tutulumlar değerlendirildi.Bulgular: Otoantikorların tanısal duyarlılık ve özgüllüğünü belirlemek için yapılan alıcı işletim karakteristiği (ROC) eğrisi analizinde en yüksek eğri altında kalan alan değeri anti-CCP-2'ye aitti. Duyarlılık ve özgüllük RF için %88 ve %90, anti-CCP-2 için %84 ve %100, anti-MCV-548 için %80 ve %90 ve anti-MCV-248 için %84 ve %100 idi. DAS-28 ile anti-CCP-2 (r=0.623), anti-MCV-548 (r=0.481) ve anti-MCV-248 (r=0.408) arasında zayıf bir korelasyon vardı. Ekstraartiküler tutulumu olan ve olmayan hastaların ve DAS-28'e göre düşük veya orta şiddette hastalık aktivitesi olan hastaların RF, anti-CCP-2, anti-MCV-548 ve anti-MCV-248 değerleri arasında istatistiksel olarak anlamlı bir fark yoktu.Sonuç: Anti-CCP, RF ve anti-MCV otoantikorları RA'nın ayırıcı tanısında faydalıdır. Fakat, anti-CCP antikoru diğer otoantikorlarla karşılaştırıldığında daha üstün bir tanısal değere sahiptir. Anti-MCV antikorunun tespitinde kullanılan anti-MCV-248 kitinin duyarlılık ve özgüllüğü anti-MCV-548 kitine göre daha yüksek olarak bulundu. Bu bulgular farklı ticari kitlerin farklı performans sergileyebileceğini akla getirmektedir. Bununla birlikte sonuçlarımızın daha fazla hastanın dahil edildiği çalışmalarla desteklenmesi gerekmektedir.
Anahtar Kelime: Konular:
Romatoid Artritte Sitrulinli Peptit Antikorlarının Tanısal Değeri: Farklı Ticari Kitlerin Karşılaştırılması
Öz: Objectives: In this study we tested the diagnostic values of rheumatoid factor (RF), anti-cyclic citrullinated peptide (anti-CCP) and two different commercial anti-mutated citrullinated vimentin (anti-MCV) kits in the differential diagnosis of rheumatoid arthritis (RA) considering that there might also be substantial differences between the performances of the commercial kits. Patients and methods: Thirty-four RA patients, admitted to our rheumatology outpatient clinics between October 2008 and February 2009, and 24 healthy controls were included in this study. Sera of RA patients and healthy controls were analyzed for RF, anti-CCP-2, anti-MCV-548 and anti-MCV-248 autoantibodies with two different commercial kits. Disease activity was determined by disease activity score 28 (DAS-28) in RA patients. Extra-articular involvement was evaluated in RA patients. Results: In the receiver operating characteristic (ROC) curve analysis perfomed to determine the diagnostic sensitivity, anti- CCP-2 had the highest value of area under ROC curve. Sensitivity and specificity was 88% and 90%, 84% and 100%, 80% and 90%, and 84% and 100% for RF, anti-CCP-2, anti-MCV-548, and anti-MCV-248 respectively. DAS-28 had a weak correlation with anti-CCP-2 (r=0.623), anti-MCV-548 (r=0.481), and Anti-MCV-248 (r=0.408). There was no statistically significant difference in RF, anti-CCP-2, anti-MCV-548, and anti-MCV-248 values between patients with or without extra-articular involvement or between patients with low or moderate disease activity according to DAS-28 score. Conclusion: Anti-CCP, RF and anti-MCV autoantibodies are all useful in the differential diagnosis of RA. However, the anti-CCP antibody has a superior diagnostic value compared to the other autoantibodies. The anti-MCV antibodies detected by the anti- MCV-248 kit seem more reliable than the anti-MCV antibodies detected by the anti-MCV-548 kit in differential diagnosis of RA due to their high sensitivity and specificity. These findings suggest that different commercial kits may exhibit different performances. Nevertheless, our results need to be confirmed by future studies which should include more patients.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Şahin Ö, KAPTANOĞLU E, BAKICI M, SEZER H, ELDEN H, HİZMETLİ S (2011). Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. , 13 - 18. |
| Chicago | Şahin Özlem,KAPTANOĞLU Ece,BAKICI Mustafa Zahir,SEZER Hafize,ELDEN Hasan,HİZMETLİ Sami Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. (2011): 13 - 18. |
| MLA | Şahin Özlem,KAPTANOĞLU Ece,BAKICI Mustafa Zahir,SEZER Hafize,ELDEN Hasan,HİZMETLİ Sami Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. , 2011, ss.13 - 18. |
| AMA | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. . 2011; 13 - 18. |
| Vancouver | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. . 2011; 13 - 18. |
| IEEE | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S "Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits." , ss.13 - 18, 2011. |
| ISNAD | Şahin, Özlem vd. "Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits". (2011), 13-18. |
| APA | Şahin Ö, KAPTANOĞLU E, BAKICI M, SEZER H, ELDEN H, HİZMETLİ S (2011). Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. Turkish Journal of Rheumatology(.)Archives of Rheumatology, 26(1), 13 - 18. |
| Chicago | Şahin Özlem,KAPTANOĞLU Ece,BAKICI Mustafa Zahir,SEZER Hafize,ELDEN Hasan,HİZMETLİ Sami Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. Turkish Journal of Rheumatology(.)Archives of Rheumatology 26, no.1 (2011): 13 - 18. |
| MLA | Şahin Özlem,KAPTANOĞLU Ece,BAKICI Mustafa Zahir,SEZER Hafize,ELDEN Hasan,HİZMETLİ Sami Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. Turkish Journal of Rheumatology(.)Archives of Rheumatology, vol.26, no.1, 2011, ss.13 - 18. |
| AMA | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. Turkish Journal of Rheumatology(.)Archives of Rheumatology. 2011; 26(1): 13 - 18. |
| Vancouver | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits. Turkish Journal of Rheumatology(.)Archives of Rheumatology. 2011; 26(1): 13 - 18. |
| IEEE | Şahin Ö,KAPTANOĞLU E,BAKICI M,SEZER H,ELDEN H,HİZMETLİ S "Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits." Turkish Journal of Rheumatology(.)Archives of Rheumatology, 26, ss.13 - 18, 2011. |
| ISNAD | Şahin, Özlem vd. "Diagnostic Value of Autoantibodies Against Citrullinated Peptide Antigens in Rheumatoid Arthritis: Comparison of Different Commercial Kits". Turkish Journal of Rheumatology(.)Archives of Rheumatology 26/1 (2011), 13-18. |
