Yıl: 2015 Cilt: 11 Sayı: 2 Sayfa Aralığı: 79 - 87 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests

Öz:
Bakteri, fungus, virus, nematod ve protozoa gibi entomopatojenik mikroorganizmalar zararlı böcek popülasyonlarının mikroorganizmaların kimyasal insektisidlere alternative olarak zararlı böceklere karşı biyolojik mücadele etmeni olarak kullanımına yol açmaktadır. Bu çalışmada, depolanmış ürün zararlılarından izole edilen farklı bakteriler ve farklı kaynaklardan izole edilen funguslar iki önemli depolanmış ürün zararlısı olan Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae) veCallosobruchus maculatus (F) (Coleoptera: Bruchidae)'a karşı laboratuar koşulları altında test edilmiştir. Laboratuar tarama testlerine göre, A. obtectus'a karşı en yüksek ölüm %73 ile bakteriler arasından Staphylococcus kloosii Fbe-10'dan ve %100 ile funguslar arasındanLecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 veBeauveria bassiana ARSEF8668'den elde edilmiştir. Funguslar arasından en yüksek mikozlanma oranı ise %100 ile Lecanicillium muscarium ARSEF3600 ve Beauveria bassiana ARSEF8668'den elde edilmiştir. C. maculatus'a karşı ise en yüksek ölüm %57 ile bakteriler arasından Bacillus pumilus Be-2'den ve %100 ile funguslar arasından Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 veBeauveria bassiana ARSEF8668'den elde edilmiştir. Funguslar arasında en yüksek mikozlanma oranı ise %90 ile Beauveria bassiana ARSEF8668'den elde edilmiştir. Bu sonuçlar bu çalışmada kullanılan fungal izolatların bakterilerden daha etkili olduğunu ve bu bakterilerin depolanmış ürün zararlılarına karşı mikrobiyal mücadele etmeni olarak geliştirilmesi amacıyla daha fazla araştırılması gerektiğini göstermektedir
Anahtar Kelime:

Depolanmış Ürün Zararlılarına Karşı Entomopatojenik Fungus ve Bakterilerin Virulansları

Öz:
Entomopathogenic microorganisms such as bacteria, fungi, viruses, nematodes and protozoa play an important role for regulation of insect pest populations and, this leads to use these microorganisms as biological control agents against pest species as an alternative to chemicals insecticides. In this study, we tested different bacteria originated from stored product pests and fungi isolated from different sources against two important stored product pests, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae) and Callosobruchus maculatus (F) (Coleoptera: Bruchidae), under laboratory conditions. Based on the laboratory screening, the highest mortality against A. obtectus within bacteria and fungi was obtained from Staphylococcus kloosii Fbe-10 with 73% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Lecanicillium muscarium ARSEF3600 and Beauveria bassiana ARSEF8668 with 100%. The highest mortality against C. maculatus within bacteria and fungi was obtained from Bacillus pumilus Be-2with 57% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Beauveria bassiana ARSEF8668 with 90%. These results showed that the fungal isolates used in this study seem to be more effective than bacteria and, should be further investigated in terms of developing microbial control agent against stored product pests
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Rees, D. Insects of Stored Products; Collingwood VIC, Csiro Publishing: Australia, 2004; 128 p.
  • Campbell, J.F.; Arthur, F.H.; Mullen, M.A. Insect Management in Food Processing Facilities. Adv. Food. Nutr. Res. 2004; 48, 240-295.
  • Rees, D. Coleoptera. In Integrated Management of Insects in Stored Products; Subramanyam, B., Hagstrum, D.W., Eds., Marcel Dekker: New York, ; 1-40. Özdem, A. Researches on the Biology of the Bean Weevil Bruchidae)] in Eskişehir. Bull. Crop. Proc.1997; 37(3-4), 118.
  • Kang, J.K., Pittendrigh, B.R., Onstad, D.W. Insect Resistance Management for Stored Product Pests: A Case Bruchidae). J. Econ. Entomol. 2013; 106(6), 2473-2490.
  • El-Aziz, S.E.A. Control Strategies of Stored Product Pests. J. Entomol. 2011; 8(2), 101-122.
  • Wakefield, M.E. In Factors affecting storage insect susceptibility to the entomopathogenic fungus Beauveria bassiana,Proceedings of the 9th Interna- tional Working Conference on Stored Product Protec- tion, Campinas, São Paulo, Brazil, Oct 15-18, 2006;
  • Lorini, I. et al., Eds.; Brazilian Post-harvest Association ABRAPOS, 2006.
  • Khetan, S.K. Microbial Pest Control. Marcel Dek- ker:NY, USA, 2001, 320 p.
  • Lacey, L.A.; Frutos, R.; Kaya, H.K.; Vail, P. Insect Pathogens as Biological Control Agents: Do They Have a Future? Biol. Cont. 2001; 21, 230-248.
  • Starnes, R.L.; Liu, C.L.; Marrone, P.G. History, Use and Future of MicrobialInsecticides. Am. Entomol. ; 39, 83-91. Koul, O.; Dhaliwal, G.S. 2002. Microbial pesti- cides: An introduction. In Microbial Pesticides; Koul,
  • O. et al., Eds., Taylor and Francisz: New York, 2002; 1
  • Lacey, L.A.; Siegel, J.P. Safety and Ecotoxicology of Entomopathogenic Bacteria. In Entomopathogenic Bacteria: from Laboratory to Field Application; Charles, J.F. et al., Eds., Kluwer Academic Publisher: Dordrecht, 2000; 253-273.
  • Entomopathogenic fungi and their role in regulation of insect populations. InComprehensive Molecular Insect Science; Gilbert, L.I. et al., Eds., Elseiver: Am- sterdam, 2005; 361-405.
  • St Leger, R.; Roberts, D.W. Engineering Improved Mycoinsecticides. Trend Biotechnol. 1997; 15, 83-86. T.
  • Sevim, A.; Donzelli, B.G.G.; Wu, D.; Demirbag, Z.; Gibson, D.M.; Turgeon, B.G. Hydrophobin Genes of the Entomopathogenic Fungus, Metarhizium Agric. For. 2014; 38, 99-110. of Plagiodera
  • Kuzina, L.V.; Peloquin, J.J.; Vacek, D.C.; Miller, T.A. Isolation andIdentification of Bacteria Associated with Adult Laboratory MexicanFruit Flies, Anastrepha ludens (Diptera: Tephritidae). Curr. Microbiol. 2001; , 290-294.
  • Gilmore, M. The Enterococci: Pathogenesis, Mo- lecular Biology, and Antibiotic Resistance. American Society for Microbiology Press: Washington DC, USA, ; 439 p. Channaiah, L.H.; Subramanyam, B.; McKinney, L.J.; Zurek, L. Stored-Product Insects Carry Antibiotic- Resistant and Potentially Virulent Enterococci. FEMS Microbiol. Ecol. 2010; 74, 464-471.
  • Hajek, A. Natural enemies: An introduction to biological control. Cambridge University Press: Cam- bridge, United Kingdom, 2004; 396 p.
  • Priest, F.G.Systematics and Ecology of Bacillus. In Bacillus subtilis and Other Gram-Positive Bacteria: Biochemistry, Physiology, and Molecular Genetics; Sonenshein, A.L. et al., Eds., ASM Press: Washington DC, 1993; 3-16.
  • Molina, C.A.; Cana-Roca, J.F.; Osuna, A.; Vilchez, S. Selection of a Bacillus pumilus Strain Highly Active against Ceratitis capitata (Wiedemann) Larvae. Appl. Environ. Microbiol. 2010; 76(5), 1320-1327.
  • Sreerag, R.S.; Jayaprakas, C.A.; Ragesh, L.; Kumar, S.N. Endosymbiotic Bacteria Associated with the Mealy Bug, Rhizoecus amorphophalli (Hemiptera: Pseudococcidae). 1155/2014/268491.
  • Molina, C.A.; Vilchez, S. Cooperation and Bacteri- al Pathogenicity: An Approach to Social Evolution. Rev. Chil. Hist. Nat. 2014; 87, 14.
  • Entomopathogenic Fungi as Effective Insect Pest Man- agement Tactic: A Review. Appl. Sci. Bus. Econ. 2014; (1), 10-18.
  • Sevim, A.; Demir, İ.; Demirbağ, Z. Molecular Characterization and Virulence of Beauveria spp. from the pityocampa Mycopathologia 2010; 170, 269-277. , DOI: Gul, H.T.; Saeed, S.; Khan, F.Z.A. Pine Processionary Moth, Thaumetopoea (Lepidoptera: Thaumetopoeidae).
APA SEVIM A, Sevim E, DEMİRCİ M (2015). Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. , 79 - 87.
Chicago SEVIM ALI,Sevim Elif,DEMİRCİ Meryem Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. (2015): 79 - 87.
MLA SEVIM ALI,Sevim Elif,DEMİRCİ Meryem Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. , 2015, ss.79 - 87.
AMA SEVIM A,Sevim E,DEMİRCİ M Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. . 2015; 79 - 87.
Vancouver SEVIM A,Sevim E,DEMİRCİ M Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. . 2015; 79 - 87.
IEEE SEVIM A,Sevim E,DEMİRCİ M "Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests." , ss.79 - 87, 2015.
ISNAD SEVIM, ALI vd. "Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests". (2015), 79-87.
APA SEVIM A, Sevim E, DEMİRCİ M (2015). Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 11(2), 79 - 87.
Chicago SEVIM ALI,Sevim Elif,DEMİRCİ Meryem Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. Celal Bayar Üniversitesi Fen Bilimleri Dergisi 11, no.2 (2015): 79 - 87.
MLA SEVIM ALI,Sevim Elif,DEMİRCİ Meryem Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. Celal Bayar Üniversitesi Fen Bilimleri Dergisi, vol.11, no.2, 2015, ss.79 - 87.
AMA SEVIM A,Sevim E,DEMİRCİ M Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. Celal Bayar Üniversitesi Fen Bilimleri Dergisi. 2015; 11(2): 79 - 87.
Vancouver SEVIM A,Sevim E,DEMİRCİ M Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests. Celal Bayar Üniversitesi Fen Bilimleri Dergisi. 2015; 11(2): 79 - 87.
IEEE SEVIM A,Sevim E,DEMİRCİ M "Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests." Celal Bayar Üniversitesi Fen Bilimleri Dergisi, 11, ss.79 - 87, 2015.
ISNAD SEVIM, ALI vd. "Virulence of Entomopathogenic Fungi and Bacteria Against Stored Product Pests". Celal Bayar Üniversitesi Fen Bilimleri Dergisi 11/2 (2015), 79-87.