Coagulase-negative staphylococci are found in the microbiota of human skin. These bacteria have been isolated from many patients samples and some of them have been accepted as causative agents. Due to the selective pressure related to anti- biotic use, especially staphylococci have developed resistance to multiple antibiotics. The aim of this study is identification of coagulase-negative staphylococci isolated from blood cultures to species level and to investigate antibiotic resistance rates. Blood cultures were sent from various clinics to the microbiology laboratory between 1 June 2013-31 May 2014 were continuously monitored by the BACTEC automated blood culture system. Preparations from the bottles giving positive signals were stained by Gram method and subcultured to Columbia agar with 5 % sheep blood and Eosin Methylene Blue agar. The identification and antibiotic susceptibility tests of bacteria that grew on these media and which were Gram positive, catalase positive and coagulase negative were carried out with VITEK 2 (bioMérieux, France) automated system. A total of 236 coagulase negative staphylococci strains were detected; 49 % were identified as Staphylococcus epider- midis, 42 % Staphylococcus hominis and 9 % Staphylococcus haemolyticus. Methicillin resistance rate of all strains was 82 %. All strains were susceptible to vancomycin, daptomycin, tigecycline and linezolid. Antibiotic resistance rates of strains were 95 % for penicillin, 81 % erythromycin, 66 % tetracycline, 61 % clindamycin, 42 % gentamicin, 38 % levofloxacin, 35 % moxifloxacin and 20 % trimethoprim-sulfamethoxazole. Antimicrobial resistance rates of coagulase negative staphylococci have been changing in recent years. Monitoring increasing resistance rates will contribute to determination of antibiotic usage policies.