Efficiacy of resveratrol and quercetin after experimental spinal cord injury

COŞAN TURGUT, Didem; DOĞANER, FULYA; VURAL, Murat; baydemir, canan; Uysal, Onur; ÇİFTÇİ, Ulvi; DELEN, EMRE

Efficiacy of resveratrol and quercetin after experimental spinal cord injury

Ulvi ÇİFTÇİ, (SB Bakırköy Dr. Sadi Konuk Eğitim ve Araştırma Hastanesi, Nöroşirürji Kliniği, İstanbul, Türkiye)

Emre DELEN, (Trakya Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Edirne, Türkiye)

Murat VURAL, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Eskişehir, Türkiye)

Onur UYSAL, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, Eskişehir, Türkiye)

Didem COŞAN TURGUT, (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Canan BAYDEMİR, (Kocaeli Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Kocaeli, Türkiye)

Fulya DOĞANER (Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Biyoloji Anabilim Dalı, Eskişehir, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

7 0

Yıl: 2016 Cilt: 22 Sayı: 5 Sayfa Aralığı: 423 - 431 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Efficiacy of resveratrol and quercetin after experimental spinal cord injury

Öz:

AMAÇ: Bu çalışmada, doğal antioksidanlardan olan resveratrol ve kuersetin'in, deneysel spinal kord travması oluşturulmuş sıçanlarda, oksidatif stres ve sekonder doku hasarı üzerine olan etkilerini araştırmak amaçlandı.GEREÇ VE YÖNTEM: Bu deneysel çalışmada, 42 adet Sprague-Dawley cinsi erkek sıçan kullanıldı. Spinal kord hasarı klip kompresyon metodu kullanılarak T4-T5 seviyesinden yapıldı. Çalışma kontrol, travma, travma+çözücü, travma+resveratol, travma+kuersetin ve travma+kombine (res-veratrol ve kuersetin) olmak üzere 6 grup üzerinden yürütüldü. İşlemden 48 saat sonra tüm sıçanlar sakrafiye edildi. Resveratrol ve kuersetin'in serum ve dokuda total antioksidan kapasite düzeyi ve paraoksonaz enzim aktivitesine ve histopatolojik bulgulara olan etkisi araştırıldı.BULGULAR: Sonuç olarak resveratrol, kuersetin ve kombine tedavi verilen gruplarda TAOK düzeyi ve PON aktivitesi değerlerinde travma grubuna göre anlamlı bir artma saptandı. Resveratrol ve kuersetin grupları arasında TAOK düzeyi ve PON aktivitesi açısından anlamlı bir farklılık yoktu. Çözücü grubunda TAOK düzeyi ile PON aktivitesi travma grubuna göre anlamlı derecede yüksekti. Histopatolojik incelemede ise sadece çözücü, resveratrol, kuersetin ve kombine tedavi gruplarında PMNL infiltrasyonunun azaldığı görüldü.TARTIŞMA: Çalışmamızdaki biyokimyasal ve histolojik değerlendirmede resveratrol ve kuersetin uygulamasının spinal kord travmasında görülen ikincil hasardan, spinal kordu korumak için kullanılabileceği sonucuna varılmıştır.

Anahtar Kelime:

Konular: Cerrahi

Deneysel spinal kord travması sonrası resveratrol ve kuersetin'in etkinliğinin araştırılması

Öz:

BACKGROUND: The aim of this study was to investigate the effect of natural antioxidants resveratrol and quercetin on oxidative stress and secondary cell damage in rats with acute spinal cord injury.METHODS: In this experimental study, 42 male Sprague-Dawley rats were used. Spinal cord injury was performed with clip compression method at level of T4-5. The study was conducted using 6 groups: control, trauma, trauma and solvent, trauma and resveratrol, trauma and quercetin, and trauma with combined resveratrol and quercetin. All rats were euthanized 48 hours after the procedure. Effects of resveratrol and quercetin on serum and tissue total antioxidant capacity and paraoxanase activity level were examined.RESULTS: Compared to trauma group, there was a significant increase in total antioxidant capacity and paraoxanase activity level in resveratrol, quercetin, and combined treatment groups. There was no significant difference between resveratrol and quercetin groups with regard to total antioxidant capacity and paraoxanase activity level. Total antioxidant capacity and paraoxanase activity level were significantly higher in solvent group than trauma group. In histopathological evaluation, there was a decrease in polymorphonuclear leukocyte infiltration in solvent, resveratrol, quercetin, and combined treatment groups.CONCLUSION: Biochemical and histological staining results of present study showed that resveratrol and quercetin may be effective in preventing secondary damage in spinal cord injury.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÇİFTÇİ U, DELEN E, VURAL M, Uysal O, COŞAN TURGUT D, baydemir c, DOĞANER F (2016). Efficiacy of resveratrol and quercetin after experimental spinal cord injury. , 423 - 431.
Chicago	ÇİFTÇİ Ulvi,DELEN EMRE,VURAL Murat,Uysal Onur,COŞAN TURGUT Didem,baydemir canan,DOĞANER FULYA Efficiacy of resveratrol and quercetin after experimental spinal cord injury. (2016): 423 - 431.
MLA	ÇİFTÇİ Ulvi,DELEN EMRE,VURAL Murat,Uysal Onur,COŞAN TURGUT Didem,baydemir canan,DOĞANER FULYA Efficiacy of resveratrol and quercetin after experimental spinal cord injury. , 2016, ss.423 - 431.
AMA	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F Efficiacy of resveratrol and quercetin after experimental spinal cord injury. . 2016; 423 - 431.
Vancouver	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F Efficiacy of resveratrol and quercetin after experimental spinal cord injury. . 2016; 423 - 431.
IEEE	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F "Efficiacy of resveratrol and quercetin after experimental spinal cord injury." , ss.423 - 431, 2016.
ISNAD	ÇİFTÇİ, Ulvi vd. "Efficiacy of resveratrol and quercetin after experimental spinal cord injury". (2016), 423-431.

APA	ÇİFTÇİ U, DELEN E, VURAL M, Uysal O, COŞAN TURGUT D, baydemir c, DOĞANER F (2016). Efficiacy of resveratrol and quercetin after experimental spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi, 22(5), 423 - 431.
Chicago	ÇİFTÇİ Ulvi,DELEN EMRE,VURAL Murat,Uysal Onur,COŞAN TURGUT Didem,baydemir canan,DOĞANER FULYA Efficiacy of resveratrol and quercetin after experimental spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi 22, no.5 (2016): 423 - 431.
MLA	ÇİFTÇİ Ulvi,DELEN EMRE,VURAL Murat,Uysal Onur,COŞAN TURGUT Didem,baydemir canan,DOĞANER FULYA Efficiacy of resveratrol and quercetin after experimental spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi, vol.22, no.5, 2016, ss.423 - 431.
AMA	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F Efficiacy of resveratrol and quercetin after experimental spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi. 2016; 22(5): 423 - 431.
Vancouver	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F Efficiacy of resveratrol and quercetin after experimental spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi. 2016; 22(5): 423 - 431.
IEEE	ÇİFTÇİ U,DELEN E,VURAL M,Uysal O,COŞAN TURGUT D,baydemir c,DOĞANER F "Efficiacy of resveratrol and quercetin after experimental spinal cord injury." Ulusal Travma ve Acil Cerrahi Dergisi, 22, ss.423 - 431, 2016.
ISNAD	ÇİFTÇİ, Ulvi vd. "Efficiacy of resveratrol and quercetin after experimental spinal cord injury". Ulusal Travma ve Acil Cerrahi Dergisi 22/5 (2016), 423-431.