The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)

Nacarkahya, Gulper; TEMİZ, Ebru; KARAGOZ, I. Didem; KILIC, Ibrahim H.; Ozaslan, Mehmet; YILMAZ, MEHMET; CENGIZ, Beyhan

The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)

Gülper NACARKAHYA, (Gaziantep Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Gaziantep, Türkiye)

MEHMET ÖZASLAN, (Gaziantep Üniversitesi, Fen Fakültesi, Moleküler Biyoloji Bölümü, Gaziantep, Türkiye)

Mehmet YILMAZ, (Gaziantep Üniversitesi, Tıp Fakültesi, Hematoloji Anabilim Dalı, Gaziantep, Türkiye)

I. Didem KARAGOZ, (Gaziantep Üniversitesi, Fen Fakültesi, Moleküler Biyoloji Bölümü, Gaziantep, Türkiye)

Ibrahim H. KILIC, (Gaziantep Üniversitesi, Fen Fakültesi, Moleküler Biyoloji Bölümü, Gaziantep, Türkiye)

Ebru TEMİZ, (Gaziantep Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Gaziantep, Türkiye)

Beyhan CENGIZ (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Ankara, Türkiye)

Uluslararası Hematoloji-Onkoloji Dergisi

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Yıl: 2016 Cilt: 26 Sayı: 2 Sayfa Aralığı: 116 - 122 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)

Öz:

Akut yyeloid lösemi (AML) bir hematopoetik kök hücre hastalığıdır. Tümör gelişimi adımların çoğu tümör büyümesinde işleyen Ca+2bağımlı sinyal yolakları tarafından düzenlenen süreçlerdir. Verapamil gibi farmakolojik ajanlar tarafından bloke edilebilir kalsiyum kanalları, hücre-içi Ca+2 homeostazının düzenlenmesinde önemlidir. AML veya diğer kanser türlerinde en önemli faktörler olarak hücre döngüsünü durdurmada ve programlanmış hücre ölümünü sağlamadaki hata, kanserin gerçek nedeni olarak kabul edilir. Bu çalışmadaki amacımız akım sitometri kullanarak AML hücre hattında, HL-60, apoptoz ve hücre döngüsüne Verapamil'in etkisini araştırmaktır. Verapamil'in etkili dozu MTT metodu ile 50 mg/ml olarak belirlendi, HL-60 hücrelerine uygulandı ve apoptoz ve hücre döngüsü açısından değerlendirildi. 50 mg/ml Verapamil uygulaması sonrasında, 24 saatin sonunda apoptotik hücre oranı %77.7 olarak saptanmıştır (p< 0.05). Hücre döngüsü analizinde, Verapamil uygulanmamış grupta Verapamil uygulanmış gruba göre, diploid hücrelerin oranı G1 fazında azalmış ve S fazında artmış ve aneuploid hücrelerin oranı G1 fazında azalmış (p< 0.05) ve S fazında artmıştır (p> 0.05)

Anahtar Kelime:

Konular: Onkoloji

AML (HL-60) Hücre Hattında Kalsiyum Kanal Blokeri Verapamilin, Hücre Döngüsü ve Apoptosiz Üzerine Etkisi

Öz:

Acute Myeloid Leukemia (AML) is a hematopoietic stem cell disorder. Many of the tumor development steps are processes regulated by Ca2+ dependent signaling pathways found in tumor growth. Calcium channels, which can be blocked by pharmacological agents such as verapamil, are important in the regulation of intracellular Ca2+ homeostasis. The failure to stop cell cycle and to achieve programmed cell death are the most important factors in AML or other types of cancers and are considered to be the real cause of cancer. Our aim in this study was to investigate the effect of verapamil on apoptosis and cell cycle in AML cell line, HL-60, using flow cytometry. The effective dose of verapamil was determined as 50 mg / ml by MTT method, and was applied to HL-60 cells, subsequently, they were assessed for apoptosis and cell cycle. After 50 mg / ml of verapamil application, apoptotic cell ratio was found to be 77.7% after 24 hours (p< 0.05). In cell cycle analysis, the ratio of diploid cells decreased in G1 phase and increased in S phase, and the ratio of aneuploid cells decreased in G1 phase (p< 0.05) and increased in S phase (p> 0.05) in verapamil-untreated group when compared to verapamil-treated group.

Anahtar Kelime:

Konular: Onkoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Nacarkahya G, Ozaslan M, YILMAZ M, KARAGOZ I, KILIC I, TEMİZ E, CENGIZ B (2016). The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). , 116 - 122.
Chicago	Nacarkahya Gulper,Ozaslan Mehmet,YILMAZ MEHMET,KARAGOZ I. Didem,KILIC Ibrahim H.,TEMİZ Ebru,CENGIZ Beyhan The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). (2016): 116 - 122.
MLA	Nacarkahya Gulper,Ozaslan Mehmet,YILMAZ MEHMET,KARAGOZ I. Didem,KILIC Ibrahim H.,TEMİZ Ebru,CENGIZ Beyhan The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). , 2016, ss.116 - 122.
AMA	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). . 2016; 116 - 122.
Vancouver	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). . 2016; 116 - 122.
IEEE	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B "The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)." , ss.116 - 122, 2016.
ISNAD	Nacarkahya, Gulper vd. "The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)". (2016), 116-122.

APA	Nacarkahya G, Ozaslan M, YILMAZ M, KARAGOZ I, KILIC I, TEMİZ E, CENGIZ B (2016). The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). Uluslararası Hematoloji-Onkoloji Dergisi, 26(2), 116 - 122.
Chicago	Nacarkahya Gulper,Ozaslan Mehmet,YILMAZ MEHMET,KARAGOZ I. Didem,KILIC Ibrahim H.,TEMİZ Ebru,CENGIZ Beyhan The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). Uluslararası Hematoloji-Onkoloji Dergisi 26, no.2 (2016): 116 - 122.
MLA	Nacarkahya Gulper,Ozaslan Mehmet,YILMAZ MEHMET,KARAGOZ I. Didem,KILIC Ibrahim H.,TEMİZ Ebru,CENGIZ Beyhan The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). Uluslararası Hematoloji-Onkoloji Dergisi, vol.26, no.2, 2016, ss.116 - 122.
AMA	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). Uluslararası Hematoloji-Onkoloji Dergisi. 2016; 26(2): 116 - 122.
Vancouver	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60). Uluslararası Hematoloji-Onkoloji Dergisi. 2016; 26(2): 116 - 122.
IEEE	Nacarkahya G,Ozaslan M,YILMAZ M,KARAGOZ I,KILIC I,TEMİZ E,CENGIZ B "The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)." Uluslararası Hematoloji-Onkoloji Dergisi, 26, ss.116 - 122, 2016.
ISNAD	Nacarkahya, Gulper vd. "The Effect of Calcium Canal Blocker, Verapamil, on Cell Cycle and Apoptosis in Acute Myeloid Leukemia Cancer Cell Line (HL-60)". Uluslararası Hematoloji-Onkoloji Dergisi 26/2 (2016), 116-122.