Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey

gücükoğlu, ali; ALİŞARLI, MUSTAFA; UYANIK, TOLGA; ÇADIRCI, Özgür; TERZİ GÜLEL, GÖKNUR

Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey

GÖKNUR TERZİ GÜLEL, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Gıda Hijyen ve Teknolojisi Anabilim Dalı, Samsun, Türkiye)

Ali GÜCÜKOĞLU, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Gıda Hijyen ve Teknolojisi Anabilim Dalı, Samsun, Türkiye)

Özgür ÇADIRCI, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Gıda Hijyen ve Teknolojisi Anabilim Dalı, Samsun, Türkiye)

Tolga UYANIK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Gıda Hijyen ve Teknolojisi Anabilim Dalı, Samsun, Türkiye)

Mustafa ALİŞARLI (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Gıda Hijyen ve Teknolojisi Anabilim Dalı, Samsun, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2015 Cilt: 39 Sayı: 2 Sayfa Aralığı: 211 - 217 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey

Öz:

The objective of the present study was to assess the presence of Listeria monocytogenes and to investigate its serotyping and antibiotic resistance in ready-to-eat (RTE) foods. A total of100 RTE foods, including 25 stuffed mussels, 25 ezme samples (a Turkish-style tomato dip/condiment), 25 fried spiced livers, and 25 mayonnaise-based salads were obtained from retail shops and supermarkets in Samsun, Turkey. Samples were analyzed using the standard procedure EN ISO 11290-1 and isolates of L. monocytogenes were confirmed for the presence of the hemolysin gene (hylA) by polymerase chain reaction. L. monocytogenes was identified in 1 of 25 (4%) mayonnaise-based salads as serotype 4b, 2 of 25 (8%) ezme samples as serotype 1/2a, and 1 of 25 (4%) fried spiced livers as serotype 4b. None of the stuffed mussel samples were found to be contaminated with L. monocytogenes. Antibiotic resistance profiles of L. monocytogenes isolates were detected by the disk diffusion method. Eight antibiotics disks were selected for resistance tests. One of the 4 isolates of L. monocytogenes was resistant to oxytetracycline and 1 isolate was resistant to vancomycin. In conclusion, the presence of L. monocytogenes serotypes 4b and 1/2a in RTE foods is of significant concern for public health as these serotypes are predominant serotypes that can cause listeriosis in humans.

Anahtar Kelime:

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Lovett J. Listeria monocytogenes. In: Doyle MP, editor. Foodborne Bacterial Pathogens. New York, NY, USA: Marcel Dekker; 1989. pp 284311.
2. Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev 1991; 55: 476511.
3. Ben Embarek PK. Presence, detection and growth of Listeria monocytogenes in seafoods: a review. Int J Food Microbiol 1994; 23: 1734.
4. Saulo AA. Ready-to-eat foods and listeriosis. Food Safety and Technology 2005; 14: 12.
5. Cartwright EJ, Jackson KA, Johnson SD, Graves LM, Silk BJ, Mahon BE. Listeriosis outbreaks and associated food vehicles, United States, 19982008.Emerg Infect Dis 2013; 19: 19.
6. Rodriguez-Lázaro D, Hernández M, Scortti M, Esteve T, Vazquez-Boland JA, Pla M. Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, iap, and lin02483 targets and ampliFluor technology. Appl Environ Microbiol 2004; 70: 13661377.
7. Schuerch DW, Wilson-Kubalek EM, Tweten RK. Molecular basis of listeriolysin O pH dependence. P Natl Acad Sci USA 2005; 102: 1253712542.
8. Poyart-Salmeron C, Carlier C, Trieu-Cuot P, Courtieu AL, Courvalin P. Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet 1990; 335: 14221426.
9. Pesavento G, Ducci B, Nieri D, Comodo N, Lo Nostro A. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control 2010; 21: 708713.
10. International Organization for Standardization. EN ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Detection and Enumeration of Listeria monocytogenes. Part 1: Detection Method. Geneva, Switzerland: International Organization for Standardization; 1997.
11. Seeliger HPR, Jones D. Listeria. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG, editors. Bergeys Manual of Systematic Bacteriology, Vol. 2. 9th ed. Baltimore, MD, USA: Williams & Wilkins; 1986. pp. 12351245.
12. Amills M, Francino O, Jansa M, Sanchez A. Isolation of genomic DNA from milk samples by using Chelex resin. J Dairy Res 1997; 64: 231238.
13. Aznar R, Alarcon B. PCR detection of Listeria monocytogenes: a study of multiple factors affecting sensitivity. J Appl Microbiol 2003; 95: 958966.
14. Mengaud J, Vicente MF, Chenevert J, Pereira JM, Geoffroy C, Gicquel-Sanzey B, Baquero F, Perez-Diaz JC, Cossart P. Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes. Infect Immun 1988; 56: 766772.
15. Doumith M, Buchrieser C, Glaser P, Jacquet C, Martin P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J Clin Microbiol 2004; 42: 38193822.
16. CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. Wayne, PA, USA: Clinical and Laboratory Standards Institute; 2010.
17. Kovacevic J, Mesak LR, Allen KJ. Occurrence and characterization of Listeria spp. in ready-to-eat retail foods from Vancouver, British Columbia. Food Microbiol 2012; 30: 372378.
18. Şireli TU, Gücükoğlu A. Prevalence and antibiotic resistance of Listeria spp. isolated from ready-to-eat foods in Ankara. Turk J Vet Anim Sci 2008; 32: 131135.
19. Di Pinto A, Novello L, Montemurro F, Bonerba E, Tantillo G. Occurrence of Listeria monocytogenes in ready-to-eat foods from supermarkets in Southern Italy. New Microbiol 2010; 33: 249252.
20. Jamali H, Chai LC, Thong KL. Detection and isolation of Listeria spp. and Listeria monocytogenes in ready-to-eat foods with various selective culture media. Food Control 2013; 32: 1924.
21. Zhang Y, Yeh E, Hall G, Cripe J, Bhagwat AA, Meng J. Characterization of Listeria monocytogenes isolated from retail foods. Int J Food Microbiol 2007; 113: 4753.
22. SantAna AS, Igarashi MC, Landgraf M, Destro MT, Franco BDGM. Prevalence, populations and pheno- and genotypic characteristics of Listeria monocytogenes isolated from ready-to-eat vegetables marketed in São Paulo, Brazil. Int J Food Microbiol 2012; 155: 19.
23. Lambertz ST, Nilsson C, Brådenmark A, Sylvén S, Johansson A, Jansson LM, Lindblad M. Prevalence and level of Listeria monocytogenes in ready-to-eat foods in Sweden 2010. Int J Food Microbiol 2012; 160: 2431.
24. Buncic S, Avery SM, Rocourt J, Dimitrijevic M. Can food-related environmental factors induce different behaviour in two key serovars, 4b and 1/2a, of Listeria monocytogenes? Int J Food Microbiol 2001; 65: 201212.
25. Ennaji H, Timinouni M, Ennaji MM, Hassar M, Cohen N. Characterization and antibiotic susceptibility of Listeria monocytogenes isolated from poultry and red meat in Morocco. Infect Drug Resist 2008; 1: 4550.
26. Marian MN, Sharifah Aminah, SM, Zuraini MI, Son R, Maimunah M, Lee HY Wong WC, Elexson N. MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia. Food Control 2012; 28: 309314.
27. Altuntas EG, Kocan D, Cosansu S, Ayhan K, Juneja VK, Materon L. Antibiotic and bacteriocin sensitivity of Listeria monocytogenes strains isolated from different foods. Food Nutr Sci 2012; 3: 363368.
28. Osaili TM, Alaboudi AR, Nesiar EA. Prevalence of Listeria spp. and antibiotic susceptibility of Listeria monocytogenes isolated from raw chicken and ready-to-eat chicken products in Jordan. Food Control 2011; 22: 586590.
29. Charpentier E, Courvalin P. Antibiotic resistance in Listeriaspp. Antimicrob Agents Ch1999; 43: 21032108.
30. Walsh D, Duffy G, Sheridan JJ, Blair IS, McDowell DA. Antibiotic resistance among Listeria, including Listeria monocytogenes, in retail foods. J Appl Microbiol 2001; 90: 517522.

APA	TERZİ GÜLEL G, gücükoğlu a, ÇADIRCI Ö, UYANIK T, ALİŞARLI M (2015). Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. , 211 - 217.
Chicago	TERZİ GÜLEL GÖKNUR,gücükoğlu ali,ÇADIRCI Özgür,UYANIK TOLGA,ALİŞARLI MUSTAFA Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. (2015): 211 - 217.
MLA	TERZİ GÜLEL GÖKNUR,gücükoğlu ali,ÇADIRCI Özgür,UYANIK TOLGA,ALİŞARLI MUSTAFA Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. , 2015, ss.211 - 217.
AMA	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. . 2015; 211 - 217.
Vancouver	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. . 2015; 211 - 217.
IEEE	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M "Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey." , ss.211 - 217, 2015.
ISNAD	TERZİ GÜLEL, GÖKNUR vd. "Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey". (2015), 211-217.

APA	TERZİ GÜLEL G, gücükoğlu a, ÇADIRCI Ö, UYANIK T, ALİŞARLI M (2015). Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. Turkish Journal of Veterinary and Animal Sciences, 39(2), 211 - 217.
Chicago	TERZİ GÜLEL GÖKNUR,gücükoğlu ali,ÇADIRCI Özgür,UYANIK TOLGA,ALİŞARLI MUSTAFA Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. Turkish Journal of Veterinary and Animal Sciences 39, no.2 (2015): 211 - 217.
MLA	TERZİ GÜLEL GÖKNUR,gücükoğlu ali,ÇADIRCI Özgür,UYANIK TOLGA,ALİŞARLI MUSTAFA Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.39, no.2, 2015, ss.211 - 217.
AMA	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2015; 39(2): 211 - 217.
Vancouver	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2015; 39(2): 211 - 217.
IEEE	TERZİ GÜLEL G,gücükoğlu a,ÇADIRCI Ö,UYANIK T,ALİŞARLI M "Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey." Turkish Journal of Veterinary and Animal Sciences, 39, ss.211 - 217, 2015.
ISNAD	TERZİ GÜLEL, GÖKNUR vd. "Serotyping and antibiotic susceptibility of Listeriamonocytogenes isolatedfrom ready-to-eat foods in Samsun, Turkey". Turkish Journal of Veterinary and Animal Sciences 39/2 (2015), 211-217.