Yıl: 2015 Cilt: 39 Sayı: 1 Sayfa Aralığı: 15 - 22 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines

Öz:
2,3'-Biindole (2) was synthesized via bromination of indole (1) with molecular bromine and underwent simultaneous dimerization. Antiproliferative and cytotoxic activity of 2 was investigated in vitro on C6 (rat brain tumor), HeLa (human cervix carcinoma), and HT29 (human colon carcinoma) cells lines by using BrdU cell proliferation ELISA and lactate dehydrogenase (LDH) assays. In contrast to 5-fluorouracil (5-FU), 2,3'-biindole (2) significantly inhibited proliferation of HeLa and HT29 cell lines. According to LDH assay, the cytotoxicity of compound 2 was low on HT29 cell lines and high on HeLa and C6 cell lines. Moreover, 2 did not cause any DNA laddering on the DNA of tested cells; therefore, it is suggested that the mechanism of action of this compound may not involve apoptosis. In addition, 2 inhibited relaxation of supercoiled plasmid DNA by topoisomerase activity. Results of the present study indicates that biindole (2) may have promising anticancer and antitopoisomerase potential with an unknown mechanism of action.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Ökten S, Erenler R, Kul Köprülü T, tekin s (2015). In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. , 15 - 22.
Chicago Ökten Salih,Erenler Ramazan,Kul Köprülü Tuğba,tekin saban In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. (2015): 15 - 22.
MLA Ökten Salih,Erenler Ramazan,Kul Köprülü Tuğba,tekin saban In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. , 2015, ss.15 - 22.
AMA Ökten S,Erenler R,Kul Köprülü T,tekin s In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. . 2015; 15 - 22.
Vancouver Ökten S,Erenler R,Kul Köprülü T,tekin s In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. . 2015; 15 - 22.
IEEE Ökten S,Erenler R,Kul Köprülü T,tekin s "In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines." , ss.15 - 22, 2015.
ISNAD Ökten, Salih vd. "In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines". (2015), 15-22.
APA Ökten S, Erenler R, Kul Köprülü T, tekin s (2015). In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. Turkish Journal of Biology, 39(1), 15 - 22.
Chicago Ökten Salih,Erenler Ramazan,Kul Köprülü Tuğba,tekin saban In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. Turkish Journal of Biology 39, no.1 (2015): 15 - 22.
MLA Ökten Salih,Erenler Ramazan,Kul Köprülü Tuğba,tekin saban In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. Turkish Journal of Biology, vol.39, no.1, 2015, ss.15 - 22.
AMA Ökten S,Erenler R,Kul Köprülü T,tekin s In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. Turkish Journal of Biology. 2015; 39(1): 15 - 22.
Vancouver Ökten S,Erenler R,Kul Köprülü T,tekin s In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines. Turkish Journal of Biology. 2015; 39(1): 15 - 22.
IEEE Ökten S,Erenler R,Kul Köprülü T,tekin s "In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines." Turkish Journal of Biology, 39, ss.15 - 22, 2015.
ISNAD Ökten, Salih vd. "In vitro antiproliferative/cytotoxic activity of 2,3'-biindole against various cancer cell lines". Turkish Journal of Biology 39/1 (2015), 15-22.