Yıl: 2015 Cilt: 12 Sayı: 1 Sayfa Aralığı: 74 - 80 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR

Öz:
Bu çalışma Kırklareli piyasasından temin edilen beyaz peynir, kaşar peynir, tereyağı, ve krema gibi çeşitli süt ürünlerinin mikrobiyolojik kalitelerinin belirlenmesi amacıyla yürütülmüştür. 79 örnekten 59 (%74,7), 54 (%68,4) ve 68 (%86,1) örnekte sırasıyla Koliformbakteri, E.colive küf/maya bulunmuştur. Staphylococcusaureus ise18 (% 22, 8) örnekte tespit edilmiştir. Listeria monoctyogenesyalnızca 1 kaşar örneğinde tespit edilirken, herhangi bir örnekte Salmonellaspp. bulgusuna rastlanılmamıştır. Yalnızca bir örnekte patojen mikroorganizma bulgusuna rastlanmasına rağmen örneklerin çoğunun mikrobiyolojik yükü Avrupa standartlarına göre izin verilen seviyenin üzerinde çıkmıştır. Bu sonuçlar ürünlerin mikrobiyolojik kalitesinin çok düşük olduğuna ve yetersiz hijyen ve sanitasyona işaret etmektedir.
Anahtar Kelime:

Konular: Ziraat Mühendisliği

Kırklareli nde Üretilen Bazı Süt Ürünlerinin Mikrobiyolojik Kalitesinin Araştırılması: Salmonellaspp. ve Listeria Monoctyogenes in Real Time PCR Kullanarak Teşhis Edilmesi

Öz:
This study was carried out for the evaluation of the microbiological quality of various dairy productsincluding white cheese, kashar cheese, butter, and milk cream purchased in retail markets in the province Kirklareli. Coliform bacteria, Escherichia coliand mold-yeast counts were investigated by the standard cultural method and found to be 59 (74.7 %) and 54 (68.4 %), and 68 (86.1 %) of the total 79 samples, respectively. Staphylococcusaureuswas detected in 18 (22.8 %) samples. Listeria monocytogenesand Salmonella spp detected by the Real Time PCR and Listeria monocytogeneswas detected in only two kashar cheese samples, while Salmonella spp. were not detected in any samples . The microbiological contamination level was higher than the permitted level according to the European standard in most of the investigated products in spite of the low level of the pathogen detection. These results also showed poor microbiological quality and inadequateproduction conditions of these products.
Anahtar Kelime:

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Akıneden Ö, Hassan AA, Schneider E and Usleber E (2008) Enterotoxigenic Properties of Staphylococcus AureusIsolated from Goats' Milk Cheese. Int J Food Microbiol. 124, 211-216.
  • Aksu H, Çolak H, Vural A and Erkan ME (1999) Diyarbakır Bölgesinde Üretilen Örgü Peynirlerde Mikrobiyolojik ve Kimyasal Özellikler Üzerine Bir Araştırma. YYU Vet Fak Derg. 10, 8-11.
  • Akyüz N, Tutşı MF, Mengel Z, Ocak E and Altun I (1998) Örgü Peynirinin Üretim Tekniği, Bazı Mikrobiyolojik Ve Kimyasal Özellikleri. V. Süt Ve Süt Ürünleri Sempozyumu-Geleneksel Süt Ürünleri. Milli Prodüktivite Merkezi Yayınları, Pp 328-337, Ankara.
  • Aman IM,Knappsteın K and Hahn G (1998) Examination Of Verotoxin Producing Escherichia Coli in Some Egyptian Dairy Products with Special Reference to Serotype O157:H7. Milchwissenschaft, 53: 676–679.
  • Andre MCDPB, Campos MRH, Borges LJ, Kıpnıs A, Pımenta FC and Serafini AB (2008) Comparison of Staphylococcus Aureus İsolates from Food Handlers, Raw Bovine Milk and Minas Frescal Cheese By Antibiogram and Pulsed-Field Gel Electrophoresis Following Smaı Digestion. Food Control 19, 200–207.
  • Anonymous (2011). Mikrobiyolojik Kriterler Tebliği, Türk Gıda Kodeksi Yönetmeliği, 29.12. 2011 tarihli 28157 sayılı Resmi Gazete, Ankara.
  • Anonymous (2013) Türkiye İstatistik Kurumu (Tüik), Temel İstatistikler, Tarım, Hayvansal Üretim, Http://www.Tuik.Gov.Tr/Ustmenu.Do?Metod=Temelist
  • Ansay SE and Kaspar CW (1997) Survey of Retail Cheeses, Dairy Processing Environments and Raw Milk for Escherichia Coli O157:H7. Lett Appl Microbiol 25, 131–134.
  • Arau ́Jo VS, Paglıares VA, Queıroz ML And Freitas-Almeıda AC (2002) Occurrence Of Staphylococcus and Enteropathogens in Soft Cheese Commercialized in The City Of Rio De Janeiro. Braz J Appl Microbiol 92, 1172–1177.
  • Arici M, Demirci M and Gündüz HH (1999) An Investigation on Listeria Spp. Contamination in White Cheese Made From Sheep’s Milk in Tekirdağ. Milchwissenschaft 54, 90-91.
  • Aygün O, Pehlivanlar S (2006) Listeria Spp. in The Raw Milk and Dairy Products in Antakya, Turkey. Food Control 17, 676-679.
  • Berrada H, Soriano JM, Picó Y and Mañes J (2008) Application of Real-Time Polymerase Chain Reaction for Rapid Determination of Salmonella In Restaurant Foods. J Rapid Meth and Auto Microbiol. 16, 299-307.
  • Beuchat LR and Ryu J H (1997) Produce Handling and Processing Practices. Emerg Infect Dis. 3, 459–465.
  • Can HY and Çelık TH (2012): Detection of Enterotoxigenic and Antimicrobial Resistant S. Aureus in Turkish Cheeses. Food Control 24, 100-103.
  • De Buyser, M.L., Dufour, B., Maıre, M. and Lafarge, V. 2001. Implication of Milk and Milk Products in Food-Borne Diseases in France and in Differentİndustrialised Countries. Int J Food Microbiol. 67, 1–17.
  • De Luca G, Zanettı F And Stampı S (1997) Staphylococcus Aureus in Dairy Products in The Bologna Area. Int J Food Microbiol. 35, 267-270.
  • Elızaquível P, Gabaldón JA Aznar R (2011) Quantification Of Salmonella Spp., Listeria Monocytogenes and Escherichia Coli O157:H7 In Non-Spiked Food Products and Evaluation of Real-Time PCR As A Diagnostic Tool in Routine Food Analysis. Food Control 22, 158-164.
  • Elmali M, Ulukanlu Z, Tuzcu M, Yaman H and Cavli P (2005) Microbiological Quality of Beef Doner Kebaps in Turkey. Archiv Lebensmittelhygiene, 56, 25-48.
  • El-SharoudWM and SpanoG (2008) Diversity and Enterotoxigenicity of Staphylococcus Spp. Associated with Domiati Cheese. J Food Protec.71, 2567-2571.
  • Erkmen O (1995) Behavior of S.Aureus In Turkish Feta Cheese During Manufacture and Ripening. J Food Protec.58, 1201-1205.
  • European Union. Commission Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs. Official Journal of European Union L 338: 1-26.
  • European Union. Commission Regulation (EC) No 1441/2007 of 5 December 2007 amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs. Official Journal of European Union L 332: 12-29.
  • Garrido A, Chapela M-J, Román B, FajardoP, Lago J, Vieites JM and Cabado AG (2013) A New Multiplex Real-Time PCR Developed Method For Salmonella Spp. and Listeria Monocytogenes Detection in Food and Environmental Samples. Food Control, 30, 76-85.
  • Gülmez M and Güven A (2001) Beyaz Ve Çeçil Peynirlerinde Campylobacter, Salmonella Ve Listeria Türlerinin Araştırılması. Kafkas Univ Vet Fak Derg. 7, 155-161.
  • Gülmez M, Oral N, Güven A, Baz E, Sezer C and Duman B (2004) Kars’ta Tüketime Sunulan Kaşar Peynirlerinin Bazı Mikrobiyolojik Ve Kimyasal Özellikleri. Kafkas Univ Vet Fak. 10, 183–188.
  • Güner A and Telli N (2011) A Survey On The Presence Of Listeria Monoctyogenes In Various Semi-Hard Cheese From Different Region of Turkey. J Anim Vet Adv. 10, 1890-1894.
  • Günsen U and Büyükyörük I (2003) Determination of Bacteriological Qualities and Aflatoxin M-1 Levels of Commercially Available Fresh Kashar Cheeses. Turk J Vet Anim Sci. 27,821-825.
  • Kayioğlu S, Yilmaz I, Demirci M and Yetim H (2003). Chemical Composition Of The Döner Samples Sold In Tekirdağ Market. Food Control. 14, 469-474.
  • LamprellH, Villard L, Chamba JF, Beuvier E, Borges E, Maurin F, Mazerolles G, Noel Y and Kodjo A. (2004) Identification and Biotyping of Coagulase Positive Staphylococci (CPS) in Ripened French Raw Milk Cheeses and Their inVitro Ability To Produce Enterotoxins. Revue De Medecine Veterinaire. 155, 92-96.
  • Le Loır Y, Baron F and Gautıer M (2003) Staphylococcus Aureus and Food Poisoning. Gmr. 2, 63-76.
  • Mccabe-Sellers BJ and Beattıe S E (2004) Emerging Trends İn Foodborne Illness Surveillance and Prevention. J Am Diet Assoc. 104, 1708-1717.
  • Mehmetoglu A C, Yaldirak G, Bodur T, Simsek M, Bozkir H, And Eren M N (2011) Incidence of Listeria Monocytogenes and Escherichia Coli O157:H7 In Two Kashar Cheese Processing Environments. Food Control. 22, 762-766.
  • Menendez S, Godinez M R, Rodriguez-Otero J L And Centeno J A (1997) Removal of Listeria Spp. In A Cheese Factory. J Food Safety. 17, 133-139.
  • O’grady J, Sedano-Balbás S, Maher M, Smiyh T And Barry T (2007) Rapid Real Time PCR Detection of Listeria Monocytogenes in Enriched Food Samples Based on The Ssra Gene, A Novel Diagnostic Target. Food Microbiol. 25, 75-84.
  • Öksuz Ö, Arici M, Kurultay Ş. And Gümüş T (2004) Incidence Of Escherichia Coli O157 in Raw Milk And White Pickled Cheese Manufactured From Raw Milk in Turkey. Food Control. 15, 453–456.
  • Özdemir S, Çelik Ş, Özdemir C And Sert S (1998) Diyarbakır’ın Karacadağ Yöresinde Mahalli Olarak Yapılan Örgü Peynirinin Mikrobiyolojik Ve Kimyasal Özellikleri. V. Süt Ve Süt Ürünleri Sempozyumu- Geleneksel Süt Ürünleri. Milli Prodüktivite Merkezi Yayınları, Pp 154-166, Ankara.
  • Schaack M M and Marth E H (1988) Behavior of Listeria Monocytogenes in Skim Milk During Fermentation With Mesophilic Lactic Starter Cultures. J Food Protect. 51, 600-606.
  • Tekinsen K K and Özdemir Z (2006) Prevalence Of Food Borne Pathogens in Turkish Van Otlu (Herb) Cheese. Food Control, 17, 707–711.
  • TondoE C, Guimarpes MCM, Henriques JAP and Ayub MAZ (2000) Assessing and Analysing Contamination of A Dairy ProductsProcessing Plant By Staphylococcus Aureus Using Antibiotic Resistance and PFGE. Can J Microbiol. 46. 1108-1114.
  • Türkoğlu H, Ceylan Z G Dayıoylu KS (2003) The Microbiological And Chemical Quality of Orgu Cheese Produced in Turkey. Pakistan J Nutr. 2, 92-94.
  • Vural A, Erkan M E and Güran H Ş (2010) The Examination of The Microbiologic Quality in Örgü Cheese (Braided Cheese) Samples. Kafkas Univ Vet Fak. 16, 53-58.
  • Walker R L, Jensen LH, Kinde H, Alexander AV and Owens LS. (1991) Environmental Survey for Listeria Species in Frozen Milk Product Plants in California. J Food Protec. 54, 178-182.
  • Yücel N and Ulusoy H (2006) A Turkey Survey Of Hygiene Indicator Bacteria and Yersinia Enterocolitica in Raw Milk and Cheese Samples. Food Control. 17, 383–388.
APA ÇETİN B, KARASU S, ATİK A, DURAK M (2015). Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. , 74 - 80.
Chicago ÇETİN B.,KARASU S.,ATİK A.,DURAK M. Z. Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. (2015): 74 - 80.
MLA ÇETİN B.,KARASU S.,ATİK A.,DURAK M. Z. Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. , 2015, ss.74 - 80.
AMA ÇETİN B,KARASU S,ATİK A,DURAK M Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. . 2015; 74 - 80.
Vancouver ÇETİN B,KARASU S,ATİK A,DURAK M Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. . 2015; 74 - 80.
IEEE ÇETİN B,KARASU S,ATİK A,DURAK M "Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR." , ss.74 - 80, 2015.
ISNAD ÇETİN, B. vd. "Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR". (2015), 74-80.
APA ÇETİN B, KARASU S, ATİK A, DURAK M (2015). Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. Tekirdağ Ziraat Fakültesi Dergisi, 12(1), 74 - 80.
Chicago ÇETİN B.,KARASU S.,ATİK A.,DURAK M. Z. Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. Tekirdağ Ziraat Fakültesi Dergisi 12, no.1 (2015): 74 - 80.
MLA ÇETİN B.,KARASU S.,ATİK A.,DURAK M. Z. Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. Tekirdağ Ziraat Fakültesi Dergisi, vol.12, no.1, 2015, ss.74 - 80.
AMA ÇETİN B,KARASU S,ATİK A,DURAK M Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. Tekirdağ Ziraat Fakültesi Dergisi. 2015; 12(1): 74 - 80.
Vancouver ÇETİN B,KARASU S,ATİK A,DURAK M Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR. Tekirdağ Ziraat Fakültesi Dergisi. 2015; 12(1): 74 - 80.
IEEE ÇETİN B,KARASU S,ATİK A,DURAK M "Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR." Tekirdağ Ziraat Fakültesi Dergisi, 12, ss.74 - 80, 2015.
ISNAD ÇETİN, B. vd. "Investigation of Microbiological Quality of Some Dairy Products in Kırklareli: Detection of Salmonella spp.and Listeria monoctyogenesby Real Time PCR". Tekirdağ Ziraat Fakültesi Dergisi 12/1 (2015), 74-80.