Yıl: 2016 Cilt: 41 Sayı: 5 Sayfa Aralığı: 338 - 346 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant

Öz:
Amaç: Biyosurfaktanlar, mikroorganizmalar tarafından üretilen yüzey aktif bileşiklerdir. Hidrokarbonla kirlenmiş çevrelerde mikroorganizmanın gelişmesi biyosurfaktan üretimini destekler. Bu çalışmada hidrokarbonca kirlenmiş topraktan izole edilen Georgenia daeguensis (giriş numarası, KP798810)'nin biyosurfaktan üretme kapasitesi çalışılmıştır.Metod: Biyosurfaktan üretim ortamının bileşenleri Plackett-Burman metodolojisi kullanılarak değerlendirilmiştir. Istatistiksel taramaya bağlı Plackett-Burman kullanılarak, üretim ortamının 11 faktörden 6'sının biyosurfaktan üretiminde önemli ölçüde etkili olduğunu ortaya çıkarılmıştır. Bulgular: Önemli faktörler; KCl, ZnSO4 .7H2 O, CaCl2 .2H2 O, KH2 PO4 , MgSO4 .7H2 O ve glukoz olarak belirlenmiştir. Bunlarına arasından KCl, %55,2 ile en yüksek katkıya sahip olmuştur. Bu şekilde gerçekleştirilen optimizasyon sonrası, kritik misel konsantrasyonu 2,2 g L-1'ye ulaşmıştır. Biyosurfaktanın antibakteriyel aktivitesi değerlendirildi- ğinde Klebsiella pneumoniae'ye karşı antibakteriyel etkiye sahip olduğu gösterilmiştir. Bunun yanısıra, biyosurfaktan FTIR, kritik misel konsantrasyonu ve zeta analizi açı- sından da karakterize edilmiştir. Sonuç: Bu çalışma Georgenia daeguensis'in, K. pneumoniae'ye karşı antibakteriyel etki sergileyen etkili bir şekilde biyosurfaktan üretebildiğini bildirmektedir.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Topraktan izole edilen bakterilerden biyosurfaktan üretiminin optimizasyonu ve surfaktanın karakterizasyonu

Öz:
Objective: Biosurfactants are surface-active substances produced by microorganisms. The growth of microorganism supports biosurfactant production on hydrocarbon polluted environments. In this study, the capacity of biosurfactant production of Georgenia daeguensis (accession number, KP798810) isolated from hydrocarbon-contaminated soil was studied.Methods: The components of biosurfactant production medium were investigated through Plackett-Burman methodology. By using Placket-Burman based on statistical screening, six of the eleven factors of production medium were found to be critically effective on the biosurfactant production.Results: The significant factors were KCl, ZnSO4.7H2O, CaCl2.2H2O, KH2PO4, MgSO4.7H2O and glucose. Among of these, KCl had the highest effect contribution with 55.22%. After the optimization by such design, critical micelle concentration value was reached to 2.2 g L-1. When anti bacterial activity of biosurfactant was screened, it showed antibacterial effect against to Klebsiella pneumoniae. Besides, the biosurfactant was characterized by FT-IR, critic micelle concentration and zeta analysis. Conclusion: This study reported that Georgenia daeguensis is able to produce effective biosurfactant exhibiting antibacterial activity against K. pneumoniae.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA TOPTAŞ Y, ÇELİKDEMİR M, TUNCER C, BURUK ŞAHİN Y, Aytar Çelik P, BURNAK N, ÇABUK A, Butun V (2016). Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. , 338 - 346.
Chicago TOPTAŞ Yağmur,ÇELİKDEMİR Meltem,TUNCER Cansel,BURUK ŞAHİN YELİZ,Aytar Çelik Pınar,BURNAK Nimetullah,ÇABUK AHMET,Butun Vural Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. (2016): 338 - 346.
MLA TOPTAŞ Yağmur,ÇELİKDEMİR Meltem,TUNCER Cansel,BURUK ŞAHİN YELİZ,Aytar Çelik Pınar,BURNAK Nimetullah,ÇABUK AHMET,Butun Vural Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. , 2016, ss.338 - 346.
AMA TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. . 2016; 338 - 346.
Vancouver TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. . 2016; 338 - 346.
IEEE TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V "Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant." , ss.338 - 346, 2016.
ISNAD TOPTAŞ, Yağmur vd. "Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant". (2016), 338-346.
APA TOPTAŞ Y, ÇELİKDEMİR M, TUNCER C, BURUK ŞAHİN Y, Aytar Çelik P, BURNAK N, ÇABUK A, Butun V (2016). Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. Türk Biyokimya Dergisi, 41(5), 338 - 346.
Chicago TOPTAŞ Yağmur,ÇELİKDEMİR Meltem,TUNCER Cansel,BURUK ŞAHİN YELİZ,Aytar Çelik Pınar,BURNAK Nimetullah,ÇABUK AHMET,Butun Vural Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. Türk Biyokimya Dergisi 41, no.5 (2016): 338 - 346.
MLA TOPTAŞ Yağmur,ÇELİKDEMİR Meltem,TUNCER Cansel,BURUK ŞAHİN YELİZ,Aytar Çelik Pınar,BURNAK Nimetullah,ÇABUK AHMET,Butun Vural Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. Türk Biyokimya Dergisi, vol.41, no.5, 2016, ss.338 - 346.
AMA TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. Türk Biyokimya Dergisi. 2016; 41(5): 338 - 346.
Vancouver TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant. Türk Biyokimya Dergisi. 2016; 41(5): 338 - 346.
IEEE TOPTAŞ Y,ÇELİKDEMİR M,TUNCER C,BURUK ŞAHİN Y,Aytar Çelik P,BURNAK N,ÇABUK A,Butun V "Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant." Türk Biyokimya Dergisi, 41, ss.338 - 346, 2016.
ISNAD TOPTAŞ, Yağmur vd. "Optimization of a biosurfactant production from bacteria isolated from soil and characterization of the surfactant". Türk Biyokimya Dergisi 41/5 (2016), 338-346.