OBJECTIVE: This descriptive research has been conducted to determine surgery nurses' perception related to organization culture. METHODS: While collecting the survey data, a "Personal Information Form" that was designed to determine the social-demographical characteristics of the nurses and the "Denison Organizational Culture Scale" which was developed by Yahyagil have been used. The survey included 72 nurses who were working in a university hospital. Frequency, percentage, arithmetic mean, Student t-test, Mann Whitney U, Kruskall Wallis and Pearson correlation analysis were used to evaluate the data.RESULTS: Mean age of the nurses was 29.70±439 years, while 79.2% of the participants were women, and 66.7% of them were undergraduates. While 54.2% of the nurses were satisfied with their job in hospital, 30.6% of them mentioned that in-house and external training activities were inadequate. However, 41.7% of them found social activities to be insufficient, and 34.7% of them specified that the institution was open to change, and 30.6% of the nurses reported that the senior managers were participant and helpful. Evaluation of the subpoints of the scale showed that the mean points of participant culture, consistency culture, harmony, mission and general scale were 28.94±5.69, 28.50±5.16, 29.05±476, 29.31±4.99 and 11485±16.53 respectively. Mission culture points of married nurses were higher than single nurses, and organization culture points of ward nurses were higher than polyclinic nurses. Mean organization, consistency and harmony points of nurses working willingly were higher than the nurses working unwillingly. These results were found to be statistically significant (p<0.05). CONCLUSION: The average organizational culture scores of nurses working in surgery wards were found to be at a favourable level. It was determined that, factors like being married, being a manager nurse, working willingly and satisfaction with the institution affected nurses positively in terms of organizational culture (p<0.05).