Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats

KİRAZ, Hasan Ali; UNAL, YUSUF; ERBATUR, Meral Erdal; AYDIN, MUHAMMED ENES; alkan, metin; Arslan, Mustafa; ÇOMU, Faruk Metin; TURGUT, Hüseyin Cihad; KİP, GÜLAY; KARTAL, SEYFİ

Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats

Hasan Ali KİRAZ, (Çanakkale Onsekiz Mart Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Çanakkale, Türkiye)

Hüseyin Cihad TURGUT, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Ağız Diş Çene Cerrahisi Anabilim Dalı, Ankara, Türkiye)

Seyfi KARTAL, (Boş)

Faruk Metin ÇOMU, (Kırıkkale Üniversitesi, Tıp Fakültesi, Psikiyatri Anabilim Dalı, Kırıkkale, Türkiye)

Gülay KİP, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Pediatri Anabilim Dalı, Ankara, Türkiye)

Metin ALKAN, (Gazi Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Ankara, Türkiye)

Muhammed Enes AYDIN, (Gazi Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Ankara, Türkiye)

Meral Erdal ERBATUR, (Gazi Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Ankara, Türkiye)

Mustafa ARSLAN, (Gazi Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Ankara, Türkiye)

Yusuf ÜNAL (Gazi Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Ankara, Türkiye)

Gazi Medical Journal

2 0

Yıl: 2015 Cilt: 26 Sayı: 3 Sayfa Aralığı: 107 - 109 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats

Öz:

Amaç: Eritrosit deformabilitesi eritrosit membranının özel yapısı ile ilgili bir fonksiyon olup, hücrenin dağılmadan oksijen taşımasına olanak sağlar. Diyabette görülen bozulmuş eritrosit deformabilitesi eritrosit agregasyonu ve mikrovasküler düzeydeki dolaşım bozukluğunun etkenlerinden biridir. Bu çalışmada sıçanlarda streptozosinle indüklenen diyabette yüksek ve düşük doz sugammadeksin eritrosit deformabilitesi üzerindeki etkisini araştırmayı amaçladık. Yöntemler: Ağırlıkları 225-300 gram arasında değişen 24 erkek Wistar albino sıçan rasgele 4 gruba ayrıldı. Grup K (kontrol; n=6), Grup DK (diyabet kontrol; n=6), Grup DR-16S (diyabet-rokuronyum-16mg sugamadeks; n=6) ve Grup DR96S (diyabet- rokuronyum-96mg sugammadeks; n=6). Kontrol ve diyabet gruplarındaki sıçanlara aynı hacimde %0.9 NaCl verildi. Diyabet oluşturmak için tek intraperitoneal enjeksiyonla 55 mg.kg-1 streptozosin (Sigma Chemical, St. Louis, MO, USA) uygulandı. Hayvanlar 30 gün süre ile izlendi ve takip süresinin sonunda kan örneklerinden eritrosit deformabilitesi ölçümü yapıldı. Bulgular: Kontrol grubundaki serum glukoz düzeyi DK, DR-16S ve DR-96S gruplarındakilerden anlamlı olarak düşük bulundu (p<0.0001). Diyabet oluşturulan sıçanlarda deformabilite indeksi anlamlı düzeyde yüksek bulundu (p<0.0001). Eritrosit deformabilitesi DR-96S grubunda Kontrol ve DK gruplarındakinden anlamlı olarak yüksek bulundu (p<0.0001 ve p=0.028). Sonuç: Bu çalışmada diyabetik sıçanlarda düşük doz sugammadeksin güvenli olduğunu gösterdik. Çalışmamazın sonuçları sugammadeksin eritrosit deformabilitesi ve mikro/makrosirkülasyon üzerindeki etkilerini araştıracak insan ve hayvan çalışmaları için yol gösterici olabilir

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Streptozosinle Diyabet Oluşturulmuş Sıçanlarda Düşük ve Yüksek Doz Sugammadeksin Eritrosit Deformabilitesi Üzerine Etkisi

Öz:

Objective: Erythrocyte deformability is a function of specially designed erythrocyte membrane properties and allows for the oxygen delivery without cell fragmentation. Impaired erythrocyte deformability in diabetes is one of the suspected factors that result in erythrocyte aggregation and the microvascular circulatory arrest. In this study, we aimed to investigate low versus high doses of sugammadex on erythrocyte deformability in streptozototocin-induced diabetic rats. Methods: Twenty-four male Wistar albino rats weighing between 225 and 300 gr were randomly divided into 4 groups. Group C (control; n=6), Group DC (diabetes sugammadex; n=6) and Group DR-96S (diabetes- rocuronium-96mg sugammadex; n=6). Rats in control and diabetes groups received a 0.9% NaCl solution at the same volume. Diabetes was induced by a single IP injection of streptozotocin (Sigma Chemical, St. Louis, MO, USA) at a dose of 55 mg.kg-1 body weight, and animals were kept alive for 30 days. At the end of the follow-up period animals' erythrocyte deformability was measured from blood samples. Results: Serum glucose was significantly lower in Group C as compared to Groups DC, DR-16S and DR-96S (p<0.0001). The deformability index was significantly increased in the diabetic rats (p<0.0001). It was significantly increased in Group DR-96S as compared to Group C and DC (p<0.0001, p=0.028, respectively). Conclusion: In this study, we showed the safety profile of low dose sugammadex in diabetic rats in terms of the erythrocyte deformability. Our findings may lead to future animal and human studies investigating sugammadex effects on erythrocyte deformability and micro/macrovascular circulation

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Chien S. Red cell deformability and its relevance to blood flow. Ann Rev Physiol 1987;49: 177-92.
Mchedlishvili G. Disturbed blood flow structuring as critical factor of hemorheological disorders in microcirculation. Clin Hemorheol Microcir 1998;19: 315-25.
Stoltz JF, Singh M, Riha P. Hemorheology in Practice (IOS Press, Amsterdam) 1999.
Mawatari S, Saito K, Murakami K, Fujino T. Absence of correlation between glycated hemoglobin and lipid composition of erythrocyte membrane in type 2 diabetic patients. Metabolism 2004;53:123-7.
Maksina AG, Mikaelian NP, Kniazev A, Dainiak BA. Structural changes in erythrocyte membranes in diabetes mellitus using spin labeled fatty acids. Biofizika 1992;37:306-9.
Jain SK, Mohandas N, Clark MR, Shohet SB. The effect of malonyldialdheide, a product of lipid peroxidation of the deformability, dehydration and Cr- survival of erythrocyte. Br J Haematol 1983;53:247-55.
Rizvi SI, Zaid MA, Anis R, Mishra N. Protective role of tea catechins against oxidation-induced damage of type 2 diabetic erythrocytes. Clin Experimen Pharmacol Physiol 2005;32:70-5.
Hayden MR, Tyagi SC, Kerklo M M, Nicolls MR. Type 2 Diabetes mellitus as a conormational disease. JOP 2005;6:287-302.
Pekpak M, Konukoğlu D, Ercan M, Erek E. Oxidative stress and trace elements in proteinuric patients. Office Journal of the Turkish Nephrology, Association 1999;4:213-8.
Schwartz RS, Madsen JW, Rybicki AC, Nagel RL. Oxidation of spectrin and deformability defects in diabetic erythrocytes. Diabetes 1991;40:701-8.
Gürbilek M, Dağlar C, Aköz M, Topçu C. Enzyme activity lipid peroxditaino and dhea(s) glucose and lipid levels in the diabetes mellitus patients. Turk J Biochem 2004;29:237-42.
Lawall H, Angelkort L. Correlation between rheological parameters and erythrocyte velocity in nailfold capillaries in patients with diabetes mellitus. Clin Hemorheol Microcirc 1999; 20:41-7.
Budak Y, Demirci H, Akdoğan M, Yavuz D. Erythrocyte membrane anionic charge in type 2 diabetic patients with retinopathy. BMC Ophthalmol 2004;4:14.
Barazzoni R, Kiwanuka E, Zanetti M, Cristini M, Vettore M, Tessari P. Insulin acutely increases fibrinogen production in individuals with type 2 diabetes but not in individuals without diabetes. Diabetes 2003; 52:1851-6.
Buder TF, Meistelman C, Raft J. Sugammadex: clinical development and practical use. Korean J Anesthesiol 2013;65:495-500.
Baldo B. Sugammadex and rocuronium-induced anaphylaxis. Anaesthesia 2012;67:1174-5
Raft J, Betala Belinga JF, Jurkolow G, Desandes E, Longrois D, Meistelman C. Clinical evaluation of post-surgical bleeding after a sugammadex injection. Ann Fr Anesth Reanim 2011;30:714-7.
Arslan M, Comu FM, Kip G, Alkan M, Kiraz HA, Ozer A, et al. Effect of dexmedetomidin on erythrocyte deformability during ischemia reperfusion injury of heart in diabetic rats. Bratisl Lek Listy 2014;115: 494-7.
Singh M, Shin S. Changes in erythrocyte aggregation and deformability in diabetes mellitus: A brief review. Indian J Exp Biol 2009;47:7-15.
Goldstein DE, Little RR, Wiedmeyer HM, England JD, McKenzie EM. Glycated hemoglobin: methodologies and clinical applications. Clin Chem 1986;32:B64-70.
Arslan M, Comu FM, Isık B, Ozturk K, Kesimer E. Effect of dexmedetomidine on erythrocyte deformability during ischemia reperfusion injury of liver in diabetic rats Bratisl Lek Listy 2012;113: 687-91.
Verando B, Fiorilli A, Croci G, Tringali C, Goi G, Mazzanti L, et al. Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients. Blood 2002;99:1064-70.
Kowluru R, Bitensky MW, Kowluru A, Dembo M, Keaton PA, Buican T. Reversible sodium pump defect and swelling in the diabetic rat erythrocyte: effect of filterability and implications for microangiopathy. Proc Natl Acad Sci (USA) 1989;86:3327-31.

APA	KİRAZ H, TURGUT H, KARTAL S, ÇOMU F, KİP G, alkan m, AYDIN M, ERBATUR M, Arslan M, UNAL Y (2015). Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. , 107 - 109.
Chicago	KİRAZ Hasan Ali,TURGUT Hüseyin Cihad,KARTAL SEYFİ,ÇOMU Faruk Metin,KİP GÜLAY,alkan metin,AYDIN MUHAMMED ENES,ERBATUR Meral Erdal,Arslan Mustafa,UNAL YUSUF Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. (2015): 107 - 109.
MLA	KİRAZ Hasan Ali,TURGUT Hüseyin Cihad,KARTAL SEYFİ,ÇOMU Faruk Metin,KİP GÜLAY,alkan metin,AYDIN MUHAMMED ENES,ERBATUR Meral Erdal,Arslan Mustafa,UNAL YUSUF Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. , 2015, ss.107 - 109.
AMA	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. . 2015; 107 - 109.
Vancouver	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. . 2015; 107 - 109.
IEEE	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y "Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats." , ss.107 - 109, 2015.
ISNAD	KİRAZ, Hasan Ali vd. "Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats". (2015), 107-109.

APA	KİRAZ H, TURGUT H, KARTAL S, ÇOMU F, KİP G, alkan m, AYDIN M, ERBATUR M, Arslan M, UNAL Y (2015). Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. Gazi Medical Journal, 26(3), 107 - 109.
Chicago	KİRAZ Hasan Ali,TURGUT Hüseyin Cihad,KARTAL SEYFİ,ÇOMU Faruk Metin,KİP GÜLAY,alkan metin,AYDIN MUHAMMED ENES,ERBATUR Meral Erdal,Arslan Mustafa,UNAL YUSUF Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. Gazi Medical Journal 26, no.3 (2015): 107 - 109.
MLA	KİRAZ Hasan Ali,TURGUT Hüseyin Cihad,KARTAL SEYFİ,ÇOMU Faruk Metin,KİP GÜLAY,alkan metin,AYDIN MUHAMMED ENES,ERBATUR Meral Erdal,Arslan Mustafa,UNAL YUSUF Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. Gazi Medical Journal, vol.26, no.3, 2015, ss.107 - 109.
AMA	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. Gazi Medical Journal. 2015; 26(3): 107 - 109.
Vancouver	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats. Gazi Medical Journal. 2015; 26(3): 107 - 109.
IEEE	KİRAZ H,TURGUT H,KARTAL S,ÇOMU F,KİP G,alkan m,AYDIN M,ERBATUR M,Arslan M,UNAL Y "Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats." Gazi Medical Journal, 26, ss.107 - 109, 2015.
ISNAD	KİRAZ, Hasan Ali vd. "Effect of Low and High Dose Sugammadex on Erythrocyte Deformability in Streptozotocin- Induced Diabetic Rats". Gazi Medical Journal 26/3 (2015), 107-109.