Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method

ÖZDEMİR, Özgün Öykü; GÜNER, AHMET; TELLİ, NİHAT; DÖNMEZ SOYER, Ferda

Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method

Nihat TELLİ, (Selçuk Üniversitesi, Teknik Bilimler Meslek Yüksekokulu, Gıda Teknolojisi Bölümü, Konya, Türkiye)

Ahmet GÜNER, (Selçuk Üniversitesi, Veteriner Fakültesi, Gıda Hijyeni ve Teknolojisi Anabilim Dalı, Konya, Türkiye)

Ferda DÖNMEZ SOYER, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Özgün Öykü ÖZDEMİR (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2016 Cilt: 40 Sayı: 5 Sayfa Aralığı: 630 - 636 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method

Öz:

This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates (3.53%) obtained from raw milk, wall/ground, and press cases were identified as L. monocytogenes via the conventional culture method and confirmed by polymerase chain reaction. These isolates were found to belong to serotype 4b. According to antibiotic resistance testing against 10 antibiotics (ampicillin, gentamicin, erythromycin, tetracycline, chloramphenicol, cefalotin, streptomycin, vancomycin, penicillin, and sulfamethoxazole/trimethoprim), it was determined that isolates from raw milk and press cases were resistant to erythromycin. PPGE band patterns of the isolates displayed indistinguishable with AscI and 80%-94% homology with ApaI. The isolates were observed to display high homology to ATCC (13932) and lower homology to EGD SLCC (5835) obtained by both enzymes.

Anahtar Kelime:

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TELLİ N, GÜNER A, DÖNMEZ SOYER F, ÖZDEMİR Ö (2016). Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. , 630 - 636.
Chicago	TELLİ NİHAT,GÜNER AHMET,DÖNMEZ SOYER Ferda,ÖZDEMİR Özgün Öykü Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. (2016): 630 - 636.
MLA	TELLİ NİHAT,GÜNER AHMET,DÖNMEZ SOYER Ferda,ÖZDEMİR Özgün Öykü Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. , 2016, ss.630 - 636.
AMA	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. . 2016; 630 - 636.
Vancouver	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. . 2016; 630 - 636.
IEEE	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö "Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method." , ss.630 - 636, 2016.
ISNAD	TELLİ, NİHAT vd. "Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method". (2016), 630-636.

APA	TELLİ N, GÜNER A, DÖNMEZ SOYER F, ÖZDEMİR Ö (2016). Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences, 40(5), 630 - 636.
Chicago	TELLİ NİHAT,GÜNER AHMET,DÖNMEZ SOYER Ferda,ÖZDEMİR Özgün Öykü Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences 40, no.5 (2016): 630 - 636.
MLA	TELLİ NİHAT,GÜNER AHMET,DÖNMEZ SOYER Ferda,ÖZDEMİR Özgün Öykü Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences, vol.40, no.5, 2016, ss.630 - 636.
AMA	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences. 2016; 40(5): 630 - 636.
Vancouver	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method. Turkish Journal of Veterinary and Animal Sciences. 2016; 40(5): 630 - 636.
IEEE	TELLİ N,GÜNER A,DÖNMEZ SOYER F,ÖZDEMİR Ö "Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method." Turkish Journal of Veterinary and Animal Sciences, 40, ss.630 - 636, 2016.
ISNAD	TELLİ, NİHAT vd. "Detection of the contamination sources of Listeriamonocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method". Turkish Journal of Veterinary and Animal Sciences 40/5 (2016), 630-636.