Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears

BEKSAÇ, Mehmet Sinan; DÖNMEZ, HANİFE GÜLER; DEMİREZEN, Sayeste

Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears

Hanife GÜLER DÖNMEZ, (Hacettepe University)

Sayeste DEMİREZEN, (Hacettepe University)

Mehmet Sinan BEKSAÇ (Hacettepe University)

GORM:Gynecology Obstetrics & Reproductive Medicine

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Yıl: 2013 Cilt: 19 Sayı: 2 Sayfa Aralığı: 91 - 95 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears

Öz:

AMAÇ: Wnt/?-katenin sinyal yolu hem hücre kaderini hem deembriyonik ve erişkin doku homeostazisini kontrol eder. Ça lış -ma mızın amacı servikal simirlerde Wnt/?-katenin sinyal yoluaktivitesinin immünositokimya kullanılarak değerlendirilmesidir.GEREÇ VE YÖNTEM: 200 hastadan alınan servikal simirler ?-katenin açısından incelendi. Membranöz boyanmayla birlikteorta (++) veya kuvvetli (+++) sitoplazmik ve/veya çekirdek boyanmasısinyal yolu aktivitesi açısından pozitif olarak kabuledildi.BULGULAR: 200 olgunun 21'inde (%10.5) Wnt/?-katenin sinyalipozitif olarak bulundu. Hiçbir hücrede çekirdek pozitifliğinerastlanmadı ancak bazı çekirdek zarları pozitifti. Ayrıca, sinyal yolu aktif durumdayken epitel hücrelerde ?-katenin'in zardakiifadesinin istatistiksel olarak anlamlı bir miktarda arttığı gözlendi.(p<0,05).SONUÇLAR: Çalışmamız rutin servikal simirlerde Wnt/?-kateninsinyal yolu aktivitesinin immünositokimyasal teknikler kullanılaraksaptanabileceğini gösterdi.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

WNT/Beta - Katenin Sinyal Aktivitesinin İmmünositokimyasal Teknik Kullanılarak Servikal Simirlerde Saptanması

Öz:

OBJECTIVES: The Wnt/?-catenin signaling pathway controls cell fate and homeostasis of embryonicand adult tissues. The aim of the study is to evaluate the activity of Wnt/?-catenin signaling in cervicalsmears by using immunocytochemistry.STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostainingof ?-catenin. The membranous staining with moderate (++) or strong (+++) cytoplasmic and/or nuclearstaining was scored positive for signaling activity.RESULTS: Positive Wnt/?-catenin signaling was found in 21 of 200 cases (10.5%). The nucleus was notfound to be positive in any of the cells. However, some nuclear membranes were positive. In addition,when signaling was active, the membranous expression of ?-catenin was observed to increase by a statisticallysignificant amount epithelial cells (p

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DÖNMEZ H, DEMİREZEN S, BEKSAÇ M (2013). Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. , 91 - 95.
Chicago	DÖNMEZ HANİFE GÜLER,DEMİREZEN Sayeste,BEKSAÇ Mehmet Sinan Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. (2013): 91 - 95.
MLA	DÖNMEZ HANİFE GÜLER,DEMİREZEN Sayeste,BEKSAÇ Mehmet Sinan Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. , 2013, ss.91 - 95.
AMA	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. . 2013; 91 - 95.
Vancouver	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. . 2013; 91 - 95.
IEEE	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M "Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears." , ss.91 - 95, 2013.
ISNAD	DÖNMEZ, HANİFE GÜLER vd. "Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears". (2013), 91-95.

APA	DÖNMEZ H, DEMİREZEN S, BEKSAÇ M (2013). Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. GORM:Gynecology Obstetrics & Reproductive Medicine, 19(2), 91 - 95.
Chicago	DÖNMEZ HANİFE GÜLER,DEMİREZEN Sayeste,BEKSAÇ Mehmet Sinan Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. GORM:Gynecology Obstetrics & Reproductive Medicine 19, no.2 (2013): 91 - 95.
MLA	DÖNMEZ HANİFE GÜLER,DEMİREZEN Sayeste,BEKSAÇ Mehmet Sinan Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. GORM:Gynecology Obstetrics & Reproductive Medicine, vol.19, no.2, 2013, ss.91 - 95.
AMA	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. GORM:Gynecology Obstetrics & Reproductive Medicine. 2013; 19(2): 91 - 95.
Vancouver	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears. GORM:Gynecology Obstetrics & Reproductive Medicine. 2013; 19(2): 91 - 95.
IEEE	DÖNMEZ H,DEMİREZEN S,BEKSAÇ M "Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears." GORM:Gynecology Obstetrics & Reproductive Medicine, 19, ss.91 - 95, 2013.
ISNAD	DÖNMEZ, HANİFE GÜLER vd. "Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears". GORM:Gynecology Obstetrics & Reproductive Medicine 19/2 (2013), 91-95.