Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase
Yıl: 2014 Cilt: 38 Sayı: 2 Sayfa Aralığı: 111 - 115 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase
Öz: Amaç: Bu çalışmanın amacı, altı farklı Anopheles maculipennis populasyonunda insektisit direnci ile bağlantılı olarak asetilkolinesteraz (AChE) duyarlılığının ve yıllık değişiminin belirlenmesidir. Yöntemler: Larva ve ergin Anopheles maculipennis örnekleri Türkiye'nin altı farklı noktasından (Birecik, Beyşehir, Çankırı, Avarız, Tatarköy ve Dereköy) toplanmıştır. Malathion ve propoxur'a karşı insektisit duyarlılığı tanımlanmıştır. AChE ve duyarsız AChE seviyeleri bireysel olarak ölçülmüştür. Bulgular: 2007 yılında malathion ve propoxur için tüm Anopheles maculipennis populasyonlarının ölüm oranları şüpheli direnç kategorisinde yer almıştır. 2008 yılında Trakya bölgesi populasyonları (Avarız, Tatarköy, Dereköy) şüpheli direnç kategorisinde yer alırken, Birecik, Beyşehir ve Çankırı populasyonları dirençli kategoride belirlenmiştir. Biyokimyasal testlerin sonuçlarına gore AChE inhibisyon oranları 2007 yılında yüksek değerdedir ve 2008 yılında Dereköy soyu hariç azalmıştır. Sonuç: Bizim sonuçlarımız malathion ve propoxura karşı insektisit direncinin 2007 yılından 2008'e arttığını ortaya çıkarmıştır. Biyokimyasal deneylerin sonuçları her iki yılda da asetilkolinesteraz duyarsızlığını ve duyarsız asetilkolinesteraz frekansının yıldan yıla arttığını göstermiştir.Bizim sonuçlarımız aynı zamanda tüm çalışılan populasyonlarda malathion ve propoxur direnci için tarımda zararlı kontrolünde yoğun olarak kullanılan organofosfat ve karbamatların, direkt sivrisinek kontrolünde kullanılanlardan daha önemli anahtar faktör olduğunu göstermiştir.
Anahtar Kelime: Konular:
Türkiye Anopheles maculipennis Meigen (Diptera: Culicidae) Populasyonlarında Malathion ve Propoxur Direnci ve Duyarsız Asetilkolinesteraz ile İlişkisi
Öz: Objective: The objective of this study was to evaluate insecticide resistance related to acetylcholinesterase (AChE) sensitivity and annual changes in An. maculipennis from six different populations.Methods: Larvae and adult samples of An. maculipennis were collected from six different localities (Birecik, Beysehir, Cankiri, Avariz, Tatarkoy, Derekoy) in Turkey. Insecticide susceptibility against malathion and propoxur was determined. AChE and insensitive AChE levels were measured individually.Results: All Anopheles maculipennis population mortality rates were placed in the suspected resistance category for malathion and propoxur in 2007. While Thrace region populations (Avariz, Tatarkoy, Derekoy) were placed in the surveillance category in 2008, the Birecik, Beysehir, and Cankiri populations were identified in the resistance category. According to the biochemical assay, AChE inhibition rates were high in 2007 and decreased in 2008, except in Derekoy.Conclusion: Our results revealed that insecticide resistance against malathion and propoxur increased from 2007 to 2008. Biochemical assay results showed that the AChE insensitivity for 2 test years and insensitive AChE frequency had increased annually. Our results also showed that extensive usage of organophosphate and carbamate for pest control in agriculture is a key factor for malathion and propoxur resistance in all tested populations rather than direct usage of mosquito control.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Akıner MM. Sivrineklerde direnç tespiti ve direnç gelişimini sağlayan enzimatik mekanizmaların araştırılması. Doktora Tezi, Hacettepe Üniversitesi Fen Bilimleri Enstitüsü; 2009.
- Akıner MM, Simsek FM, Caglar. Insecticide resistance of Culex pip- iens (Diptera: Culicidae) in Turkey. J Pestic Sci 2009; 34: 259-64. [CrossRef]
- Akıner MM, Caglar SS, Simsek FM. Yearly changes of insecticide susceptibility and possible insecticide resistance mechanisms of Anopheles maculipennis Meigen (Diptera: Culicidae) in Turkey. Acta Trop 2013; 126: 280-5. [CrossRef]
- Becker N, Petric D, Zgomba M, Boase C, Dahl C, Lane J, Kaiser A. Mosquitoes and their control. Kluwer Academic, Plenum publishers; 2003. [CrossRef]
- Bourguet D, Capela R, Raymond M. An insensitive actylcholin- esterase in Culex pipiens (Diptera: Culicidae) from Portugal. J Econ Entomol 1996; 89: 1060-6.
- Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of pro- tein-dye binding. Anal Biochem 1976; 72: 248-54. [CrossRef]
- Bruce-Chwatt L, de Zulueta J. The rise and fall of malaria in Europe. A-historico-epidemiological study, Oxford: University Press; 1980
- Corbett JR. The biochemical mode of action of pesticides. London: Academic Press; 1974.
- Cui F, Raymond M, Berthomieu A, Alout H, Weill M, Qiao CL. Recent emergence of insensitive acetylcholinesterase in Chinese populations of the mosquito Culex pipiens (Diptera: Culicidae). J Med Entomol 2006; 43: 878-83. [CrossRef]
- Etang J, Manga L, Toto JC, Guillet P, Fondjo E, Chandre F. Spectrum of metabolic-based resistance to DDT and pyrethroids in Anopheles gam- biae s.l. populations from Cameroon. J Vector Ecol 2007; 32: 123-33. [CrossRef]
- Hemingway J, Malcolm CA, Kissoon KE, Boddington RG, Curtis CF, Hill N. The biochemistry of insecticide resistance in An. sacharovi: Comparative studies with a range of insecticide susceptible and resistance Anopheles and Culex species. Pesticide Biochem and Physiol 1985; 24: 68-76. [CrossRef]
- Hemingway J, Ranson H. Insecticide resistance in insect vector of human disease. Ann Rev Entomol 2000; 45: 375-91. [CrossRef]
- Hemingway J, Hawkes NJ, McCarroll L, Ranson H. The molecular basis of insecticide resistance in mosquitoes. Insect Biochem Mol Biol 2004; 34: 653-65. [CrossRef]
- Jetten TH, Takken W. Anophelism without malaria in Europe -a review of the ecology and distribution of the genus Anopheles in Europe. Wageningen Agricultural University Papers 1994; 94-5.
- Kasap H, Luleyap U, Alptekin D, Kasap M. Use of insecticides in Cukurova and development of resistance in mosquitoes. Acta Par Turcica 1999; 23: 267-72.
- Kasap H, Kasap M, Alptekin D, Luleyap U, Herath PR. Insecticide resistance in Anopheles sacharovi Favre in southern Turkey. Bull World Health Organ 2000; 78: 687-92.
- Linton YM, Smith L, Harbach RE. Molecular confirmation of sym- patric populations of Anopheles messeae and Anopheles atropar- vus overwintering in Kent, southeast England. European Mosq. Bull 2002; 13: 8-16.
- Luleyap U, Kasap H. Insecticide resistance in malaria vector An. sacharovi. Turk J Biol 2000; 24: 437-60.
- Mayima A, Ishikava Y, Kono Y. Actylcholinesterase in insecticide resistant Culex tritaeniorhyncus: characteristics accompanying insensitive to inhibitors. App Entomol Zool 1997; 32: 37-44.
- Nebeshima T, Mori A, Kozaki T, Iwata Y, Hidoh O, Harada S, et al. An amino acid sustititon attribute to insecticide-insensitivity of acetyl- cholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhyncus. Biochem Biophys Res Commun 2004; 313: 794-801. [CrossRef]
- Ramsdale CD, Herath P, Davidson G. Recent developments of insecticide resistance in some Turkish anophelines. J Trop Med and Hyg 1980; 83: 11-9.
- Ramsdale CD, Alten B, Çaglar SS, Ozer N. A revised, annotated checklist of the mosquitoes (Diptera: Culicidae) of Turkey. Euro Mosq Bull 2001; 9: 18-28.
- Raymond M, Fournier D, Bride JM, Cuany A, Berge J, Magnin M, et al. Identification of resistance mechanisms in Culex pipiens (Diptera: Culicidae) from southern France: insensitive acetylcholinesterase and detoxifying oxidases. J Econ Entomol 1986; 79: 1452-8.
- Sedaghat MM, Linton YM, Nicolescu G, Smith L, Koliopoulos G, Zounos AK, et al. Morphological and molecular characterization of Anopheles (Anopheles) sacharovi Favre, a primary vector of malaria in the Middle East. Syst Entomol 2003; 28: 241-56. [CrossRef]
- Simsek FM, Ulger C, Akiner MM, Tuncay SS, Kiremit F, Bardakci F. Molecular identification and distribution of Anopheles maculipen- nis complex in the Mediterranean region of Turkey. Biochem System and Ecol 2011; 39: 258-65. [CrossRef]
- Tantley ML, Tortosa P, Alout H, Berticat C, Berthomieu A, Rutee A, et al. Insecticide resistance in Culex pipiens quinquefasciatus and Aedes albopictus mosquitoes from La Réunion Island. Insect Biochem Mol Biol 2010; 40: 317-24. [CrossRef]
- Toutant JP. Insect acetylcholinesterase: catalytic properties, tissue distribution and molecular forms. Progr Neurobiol 1989; 32: 423-46. [CrossRef]
- Weill M, Lutfalla G, Mogensen K, Chandre F, Berthomieu A, Berticat C, et al. Comparative genomics: Insecticide resistance in mosquito vectors. Nature 2003; 423: 136-7. [CrossRef]
- Weill M, Malcolm C, Chandre F, Mogensen K, Berthomieu A, Marquine M, et al. The unique mutation in ace-1 giving high insec- ticide resistance is easily detectable in mosquito vectors. Insect Mol Biol 2004; 13: 1-7. [CrossRef]
- WHO (World Health Organization). Instructions for determining the susceptibility or resistance of adult mosquito to organochlorine, organophosphate and carbamate insecticides-diagnostic tests. 1981; WHO/VBC/81.806.
- WHO (World Health Organization), Techniques to detect insecticide resistance mechanisms (field and laboratory manual). 1998; WHO/ CDS/CPC/MAL/98.6
| APA | AKINER M (2014). Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. , 111 - 115. |
| Chicago | AKINER Muhammet Mustafa Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. (2014): 111 - 115. |
| MLA | AKINER Muhammet Mustafa Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. , 2014, ss.111 - 115. |
| AMA | AKINER M Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. . 2014; 111 - 115. |
| Vancouver | AKINER M Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. . 2014; 111 - 115. |
| IEEE | AKINER M "Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase." , ss.111 - 115, 2014. |
| ISNAD | AKINER, Muhammet Mustafa. "Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase". (2014), 111-115. |
| APA | AKINER M (2014). Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. Türkiye Parazitoloji Dergisi, 38(2), 111 - 115. |
| Chicago | AKINER Muhammet Mustafa Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. Türkiye Parazitoloji Dergisi 38, no.2 (2014): 111 - 115. |
| MLA | AKINER Muhammet Mustafa Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. Türkiye Parazitoloji Dergisi, vol.38, no.2, 2014, ss.111 - 115. |
| AMA | AKINER M Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. Türkiye Parazitoloji Dergisi. 2014; 38(2): 111 - 115. |
| Vancouver | AKINER M Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase. Türkiye Parazitoloji Dergisi. 2014; 38(2): 111 - 115. |
| IEEE | AKINER M "Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase." Türkiye Parazitoloji Dergisi, 38, ss.111 - 115, 2014. |
| ISNAD | AKINER, Muhammet Mustafa. "Malathion and Propoxur Resistance in Turkish Populations of the Anopheles maculipennis Meigen (Diptera: Culicidae) and Relation to the Insensitive Acetylcholinesterase". Türkiye Parazitoloji Dergisi 38/2 (2014), 111-115. |
