Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri

Oğuz Ayhan KİREÇCİ (Bitlis Eren Üniversitesi Hizan MYO, Bitkisel ve Hayvansal Üretim Bölümü, BİTLİS)
Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi
1 0
Yıl: 2017 Cilt: 20 Sayı: 3 Sayfa Aralığı: 175 - 184 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri

Öz:
Ağır metaller; düşük konsantrasyonlarda bile toksik veya zehirleyici olabilen yüksek yoğunluklu metallerdir. Ağır metaller belirli bir zaman aralığında canlı organizmada diğer metallere oranla daha fazla birikir. Sonuç olarak giderek artan olumsuz etkilere sebep olur. Araştırmada kullanılan Saccharomyces cerevisiae metal biyo absorbsiyonu çalışmalarında sıklıkla kullanılmaktadır. S. cerevisiae ile yapılan bu çalışma ile ilk kez Mn, Mg, Cd ve Fe metal iyonlarının lipit peroksidasyonu (MDA), glutatyon (GSH), yağ asidi ve vitamin içerikleri gibi bazı biyokimyasal parametreler üzerine etkileri araştırılmıştır. Deneyde kullanılan S. cerevisiae FMC16'nın gelişimi ve çoğalması için YEDP besiyeri ortamı hazırlandı. Uygulama grupları için; Mn, Mg, Cd ve Fe ağır metallerinin her birinden 2 mg 2001 mL olacak şekilde kültür ortamına ilave edildi. Elde edilen süpernatan ile, GSH (Elman reaktifi ile), total protein (Lowry yöntemine göre) ve MDA (Ohkawa ve ark.'nın spektrofometrik yöntemine göre), yağ asitleri (Christie'ye göre) ve vitamin (Katsanidis ve Addis'e göre) analizleri yapıldı. Sonuç olarak, ağır metal uygulamasının MDA ve GSH içeriklerini önemli seviyede artırdığı ve bu artışların istatistiksel açıdan da önemli olduğu (p<0.0001) belirlenmiştir. Buna karşılık; ağır metallerin yağ asidi ve vitamin içeriği üzerine etkilerinin oldukça değişken olduğu anlaşılmıştır. S. cerevisiae'dan elde edilen bu sonuçlar ekosistemi oluşturan bütün ökaryotik organizmalara uyarlandığında, özellikle çevrede ve besinlerde birikimi oldukça yüksek olan bu ağır metallerin tüketimine dikkat edilmesi gerektiği sonucuna ulaşılmaktadır
Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji

Effect of Heavy Metals (Manganese, Magnesium, Cadmium, and Iron) added Saccharomyces cerevisiae Culture Medium on Some Biochemical Parameters

Öz:
Heavy metals are high density metals that can be toxic even at low concentrations. Heavy metals accumulate in living organisms at a greater rate than other metals in a certain time interval. As a consequence, they result gradually increasing negative effects. Saccharomyces cerevisiae used in this study is frequently preferred in metal bioabsorption. The effects of Mn, Mg, Cd and Fe metal ions on some biochemical parameters such as lipid peroxidation (MDA), glutathione (GSH), fatty acid and vitamin contents were investigated for the first time in this study with S. cerevisiae. For the development and proliferation of S. cerevisiae FMC16 in the study, YEDP (1 g yeast extract for 100 ml, 2 g bactopeptone, 2 g glucose) medium was used. For application groups; 2 mg 200-1 mL of each of heavy metals including Mn, Mg, Cd and Fe were added to the culture medium. GSH (with Elman Reactivity), total protein (according to Lowry) and MDA (according to the spectrophotometric method of Ohkawa et al.), fatty acid (according to Christie) and vitamins (according to Katsanidis and Addis) analyzes were performed with the obtained supernatant. Results indicated that heavy metal application increased MDA and GSH contents significantly (p <0.0001). Whereas, it is concluded that heavy metals have various effects on fatty acids and vitamin content and are accumulating a high degree in environment and organisms. Thus, all living organisms should avoid consuming any of these heavy metals.
Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA kirecci o (2017). Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. , 175 - 184.
Chicago kirecci oguz ayhan Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. (2017): 175 - 184.
MLA kirecci oguz ayhan Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. , 2017, ss.175 - 184.
AMA kirecci o Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. . 2017; 175 - 184.
Vancouver kirecci o Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. . 2017; 175 - 184.
IEEE kirecci o "Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri." , ss.175 - 184, 2017.
ISNAD kirecci, oguz ayhan. "Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri". (2017), 175-184.
APA kirecci o (2017). Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 20(3), 175 - 184.
Chicago kirecci oguz ayhan Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 20, no.3 (2017): 175 - 184.
MLA kirecci oguz ayhan Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, vol.20, no.3, 2017, ss.175 - 184.
AMA kirecci o Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi. 2017; 20(3): 175 - 184.
Vancouver kirecci o Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi. 2017; 20(3): 175 - 184.
IEEE kirecci o "Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri." Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 20, ss.175 - 184, 2017.
ISNAD kirecci, oguz ayhan. "Saccharomyces cerevisiae’nın Gelişme Ortamına İlave Edilen Ağır Metallerin (Mn, Mg, Cd, Fe) Bazı Biyokimyasal Parametrelere Etkileri". Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi 20/3 (2017), 175-184.
Sayfa Oluşturulma Tarihi
23-04-2024 1:46

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