VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis

YÜCE, Salim; UYSAL, İsmail Önder; DOĞAN, Mansur; SEZGİN, İlhan; CANDAN, Ferhan; BAĞCI, Binnur; KOÇ, Sema; Bağcı, Gökhan

VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis

Salim YÜCE, (Cumhuriyet Üniversitesi, Tıp Fakültesi, Kulak Burun Boğaz Anabilim Dalı, Sivas, Türkiye)

Binnur BAĞCI, (Cumhuriyet Üniversitesi, Sağlık Bilimleri Fakültesi, Sivas, Türkiye)

Gökhan BAĞCI, (Cumhuriyet Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Sivas, Türkiye)

Mansur DOĞAN, (Cumhuriyet Üniversitesi, Tıp Fakültesi, Kulak Burun Boğaz Anabilim Dalı, Sivas, Türkiye)

Sema KOÇ, (Antalya Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Hastalıkları Kliniği, Antalya, Türkiye)

İlhan SEZGİN, (Cumhuriyet Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Sivas, Türkiye)

Ferhan CANDAN, (Cumhuriyet Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Sivas, Türkiye)

İsmail Önder UYSAL (Cumhuriyet Üniversitesi, Tıp Fakültesi, Kulak Burun Boğaz Anabilim Dalı, Sivas, Türkiye)

Journal of Clinical and Analytical Medicine

1 0

Yıl: 2016 Cilt: 7 Sayı: 5 Sayfa Aralığı: 701 - 705 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis

Öz:

Amaç: Rekürren aftöz stomatit (RAS) oral mukozanın en sık görülen hastalıklarından biridir ve kronik, ağrılı, tekrarlayan ve nekrotizan ülserasyonlarla karakterizedir. RAS'ın kesin etiyolojisi ve patogenezi açıklanamamıştır. Bu yüzden, Türk RAS'lı hastalarda serum VEGF, sVEGFR-1 ve endostatin düzeylerini; ve ayıca VEGF +936 C/T ve -1154 G/A tek nükleotid polimorfizmlerinin (SNP'ler) frekanslarını araştırmayı amaçladık. Gereç ve Yöntem: 42 RAS'lı hasta (24 minör RAS ve 18 majör RAS) ve 37 sağlıklı birey çalışmaya dahil edildi. Serum VEGF, sVEGFR-1 ve endostatin düzeyleri ELİZA yöntemiyle ölçüldü. VEGF +936 C/T ve -1154 G/A SNP'leri PCR-RFLP metoduyla tanımlandı. Bulgular: VEGF serum düzeyi ortalaması +936 C/T SNP'sinin CC genotipini taşıyanlarda CT genotipini taşıyanlara göre daha yüksek bulundu (639.5 ± 309.1 vs 442.1 ± 197.8; p = 0.032). VEGF -1154 GA ve GG genotipleri sırasıyla minor RAS ve major RAS'lı hastalarda daha sık olarak bulundu (p = 0.022). Minor RAS ile major arasında serum VEGF düzeyi ortalaması açısından anlamlı bir farklılık vardı. (677.1 ± 316.7 vs 492.9 ± 242.7; p = 0.032). Tartışma: +936 C/T SNP'sinin T alleli, azalmış serum VEGF düzeyi ile ilişkilidir ve bu azalma, RAS etiyolojisinde ve patogenezindeki bozulmuş neovaskülarizasyon ve reepitelizayon üzerinde yardımcı bir role sahip olabilir. VEGF'nin RAS yatkınlığı ve onun klinik göstergelerindeki rolünü belirlemek için daha fazla çalışmaya ihtiyaç duyulmaktadır.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Rekürren Aftöz Stomatitte VEGF, sVEGFR-1 ve Endostatin Serum Düzeyleri ile VEGF Polimorfizmleri

Öz:

Aim: Recurrent aphthous stomatitis (RAS) is one of the most frequent diseases of the oral mucosa, characterized by chronic, painful, recurrent, and necrotizing ulcerations. The precise etiology and pathogenesis of RAS have not been clarified. Therefore, we aimed to investigate serum levels of VEGF, sVEGFR-1, and endostatin as well as the frequencies of VEGF +936 C/T and -1154 G/A single nucleotide polymorphisms (SNPs) in Turkish patients with recurrent aphthous stomatitis. Material and Method: Forty-two patients with RAS (24 minor RAS and 18 major RAS) and 37 healthy subjects were included in the study. Serum levels of VEGF, sVEGFR-1, and endostatin were measured using the ELISA method. VEGF +936 C/T and -1154 G/A SNPs were determined by the PCR-RFLP method. Results: The mean serum level of VEGF was found higher in bearing CC genotype of +936 C/T SNP compared with CT genotype (639.5 ± 309.1 vs 442.1 ± 197.8; p = 0.032). VEGF -1154 GA genotype was found to be more frequent in patients with minor RAS and GG genotype was more frequent in patients with major RAS (p = 0.022). There was a significant difference between minor RAS and major RAS with regard to mean VEGF serum levels (677.1 ± 316.7 vs 492.9 ± 242.7; p = 0.032).Discussion: The T allele of +936 C/T SNP is associated with decreased serum VEGF level, and this decrease may have a contributory role in impaired neovascularization and re-epithelialization in the etiology and pathogenesis of RAS. Further studies are needed to determine the role of VEGF in RAS susceptibility and its clinical manifestations

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YÜCE S, BAĞCI B, Bağcı G, DOĞAN M, KOÇ S, SEZGİN İ, CANDAN F, UYSAL İ (2016). VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. , 701 - 705.
Chicago	YÜCE Salim,BAĞCI Binnur,Bağcı Gökhan,DOĞAN Mansur,KOÇ Sema,SEZGİN İlhan,CANDAN Ferhan,UYSAL İsmail Önder VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. (2016): 701 - 705.
MLA	YÜCE Salim,BAĞCI Binnur,Bağcı Gökhan,DOĞAN Mansur,KOÇ Sema,SEZGİN İlhan,CANDAN Ferhan,UYSAL İsmail Önder VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. , 2016, ss.701 - 705.
AMA	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. . 2016; 701 - 705.
Vancouver	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. . 2016; 701 - 705.
IEEE	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ "VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis." , ss.701 - 705, 2016.
ISNAD	YÜCE, Salim vd. "VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis". (2016), 701-705.

APA	YÜCE S, BAĞCI B, Bağcı G, DOĞAN M, KOÇ S, SEZGİN İ, CANDAN F, UYSAL İ (2016). VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. Journal of Clinical and Analytical Medicine, 7(5), 701 - 705.
Chicago	YÜCE Salim,BAĞCI Binnur,Bağcı Gökhan,DOĞAN Mansur,KOÇ Sema,SEZGİN İlhan,CANDAN Ferhan,UYSAL İsmail Önder VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. Journal of Clinical and Analytical Medicine 7, no.5 (2016): 701 - 705.
MLA	YÜCE Salim,BAĞCI Binnur,Bağcı Gökhan,DOĞAN Mansur,KOÇ Sema,SEZGİN İlhan,CANDAN Ferhan,UYSAL İsmail Önder VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. Journal of Clinical and Analytical Medicine, vol.7, no.5, 2016, ss.701 - 705.
AMA	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. Journal of Clinical and Analytical Medicine. 2016; 7(5): 701 - 705.
Vancouver	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis. Journal of Clinical and Analytical Medicine. 2016; 7(5): 701 - 705.
IEEE	YÜCE S,BAĞCI B,Bağcı G,DOĞAN M,KOÇ S,SEZGİN İ,CANDAN F,UYSAL İ "VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis." Journal of Clinical and Analytical Medicine, 7, ss.701 - 705, 2016.
ISNAD	YÜCE, Salim vd. "VEGF, sVEGFR-1 and Endostatin Serum Levels and VEGF Polymorphisms in Recurrent Aphthous Stomatitis". Journal of Clinical and Analytical Medicine 7/5 (2016), 701-705.