N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri

ÖZCAN, Şaziye; APAN, Alpaslan; GÜLTEKİN, Süreyya

N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri

Şaziye ÖZCAN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kırıkkale, Türkiye)

Alpaslan APAN, (Kırıkkale Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kırıkkale, Türkiye)

Süreyya GÜLTEKİN (Kırıkkale Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kırıkkale, Türkiye)

Türk Anestezi ve Reanimasyon Dergisi

5 0

Yıl: 2003 Cilt: 31 Sayı: 10 Sayfa Aralığı: 511 - 515 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri

Öz:

N-Metil-D-Aspartat reseptörü kalsiyum ile aktif hale gelmektedir. Kalsiyum antagonisti verapamil ve N-metil-D-aspartat antagonisti MK-801'in sıçanlarda kronik sinir hasarı modelinde termal nosisepsiyona olan etkileri bilinmektedir. Bu çalışmada her iki ilacın birlikte uygulanmasının analjezik etkinliğinin araştırılması amaçlandı. Çalışma, ağırlıkları 180-220 g arasında değişen Sprague Dawley cinsi 30 erkek sıçanda gerçekleştirildi. Barbitürat anestezisi altında sağ siyatik sinirleri bulunarak 4/0 kromik katgüt ile gevşek şekilde bağlandı. İlaçlara ameliyatı takiben başlandı ve beş gün süre ile uygulandı. Tedavi protokolünde: ilk dört gün intraperitoneal 10 mg kg-1 verapamil uygulandı; beşinci gün ise 20 dakikalık ara ile verapamil 1 mg kg-1 MK-801 ile kombine edildi. Nosiseptif testler 46.5°C'de Hot Plate Test ile 120 dakika boyunca her 30 dakikada bir ölçüm yapılarak gerçekleştirildi. Opere bacağın çekilme zamanı reaksiyon süresi olarak belirlenerek kaydedildi. Nöropatik ağrı modelinde kronik verapamil veya tek başına MK-801 uygulaması nosisepsiyonu belirgin ölçüde azalttı. İki ilacın kombinasyonu ile tek başına gözlenen antinosiseptif etkinin altında değerler elde edildi. Kalsiyum antagonisti ön tedavisi nöropatik ağrı mode-linde N-metil-D-aspartat reseptörüne olan duyarlılığı azaltmaktadır. N-metil-D-aspartat reseptöründe kalsiyum antagonistleri ve MK-801'in bağlanma bölgeleri birbirine yakın olabilir. Bu nedenle, birlikte uygulandıklarında reseptör duyarlılığını azaltarak ve/veya bağlanma düzeyinde etkileşerek antagonist etkinin görülmesine neden olduklarını düşünmekteyiz.

Anahtar Kelime: Kalsiyum kanal blokörleri Analjezi N-metilaspartat Modeller, hayvan Verapamil Sıçan, Sprague-Dawley

Konular: Cerrahi

The effects of combination of verapamil and MK-801 on rat neuropathic pain model

Öz:

N-methly-D-aspartate receptor is activated by calcium. The effects of a calcium antagonist verapamil and N-methly-D-aspartae receptor antagonist MK-801 on thermal nociception in rat model of chronic nerve injury was well known. We aimed to study the analgesic effects of combination of two drugs using the same model. The present study was constituted on 30 male Sprague Dawley rat weighing 180-220 g. The right common sciatic nerve was exposed and ligated loosely with 4/0 chromic gut under anaesthesia with a barbiturate. The administration of drugs were started after the operation and continued through five days. According to the treatment protocol, verapamil 10 mg kg-1 was administered for four days and on the fifth day MK-801 1 mg kg-1 was combined. The drugs were given with 20 mi-nutes' intervals through intraperitoneal route. Nociceptive tests were performed by Hot Plate Test at 46.5°C throughout a 120 minutes' period at every 30 minutes. The time of withdrawal of the operated limb was accepted as reaction time. Chronic administration of verapamil or MK-801 admi-nistration as a sole drug was substantially reduced nociception on neuropathic pain model. The antinociceptive effects of the combination of drugs was found to be lower than values obtained from each drug individually. Pretreatment with a calcium antagonist reduced the sensitivity to N-methyl-D-aspartate receptor on neuropthic pain model. It was concluded that, the binding sites of calcium antagonists and MK-801 on N-methyl-D-aspartate receptors can be close to each other, therefore antagonism may be seen by reducing receptor sensitivity or a reaction during binding.

Anahtar Kelime: Calcium Channel Blockers Analgesia N-Methylaspartate Models, Animal Verapamil Rats, Sprague-Dawley

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZCAN Ş, APAN A, GÜLTEKİN S (2003). N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. , 511 - 515.
Chicago	ÖZCAN Şaziye,APAN Alpaslan,GÜLTEKİN Süreyya N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. (2003): 511 - 515.
MLA	ÖZCAN Şaziye,APAN Alpaslan,GÜLTEKİN Süreyya N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. , 2003, ss.511 - 515.
AMA	ÖZCAN Ş,APAN A,GÜLTEKİN S N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. . 2003; 511 - 515.
Vancouver	ÖZCAN Ş,APAN A,GÜLTEKİN S N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. . 2003; 511 - 515.
IEEE	ÖZCAN Ş,APAN A,GÜLTEKİN S "N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri." , ss.511 - 515, 2003.
ISNAD	ÖZCAN, Şaziye vd. "N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri". (2003), 511-515.

APA	ÖZCAN Ş, APAN A, GÜLTEKİN S (2003). N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. Türk Anestezi ve Reanimasyon Dergisi, 31(10), 511 - 515.
Chicago	ÖZCAN Şaziye,APAN Alpaslan,GÜLTEKİN Süreyya N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. Türk Anestezi ve Reanimasyon Dergisi 31, no.10 (2003): 511 - 515.
MLA	ÖZCAN Şaziye,APAN Alpaslan,GÜLTEKİN Süreyya N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. Türk Anestezi ve Reanimasyon Dergisi, vol.31, no.10, 2003, ss.511 - 515.
AMA	ÖZCAN Ş,APAN A,GÜLTEKİN S N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. Türk Anestezi ve Reanimasyon Dergisi. 2003; 31(10): 511 - 515.
Vancouver	ÖZCAN Ş,APAN A,GÜLTEKİN S N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri. Türk Anestezi ve Reanimasyon Dergisi. 2003; 31(10): 511 - 515.
IEEE	ÖZCAN Ş,APAN A,GÜLTEKİN S "N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri." Türk Anestezi ve Reanimasyon Dergisi, 31, ss.511 - 515, 2003.
ISNAD	ÖZCAN, Şaziye vd. "N-Metil-D-Aspartat antagonistik MK-801 ve verapamil kombinasyonunun mononöropatik ağrı modeli üzerine etkileri". Türk Anestezi ve Reanimasyon Dergisi 31/10 (2003), 511-515.