Yıl: 2016 Cilt: 16 Sayı: 12 Sayfa Aralığı: 931 - 939 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk

Öz:
Objective: Atherosclerotic coronary artery disease (CAD) appears to be a multifactorial process caused by the interaction of environmental risk factors with multiple predisposing genes. Therefore, in this study we aimed to determine the role of oxidative DNA damage and some variations in glutathione S-transferase (GSTM1 and GSTT1) and DNA repair (hOGG1) genes in CAD risk.Methods: A case-control study was conducted on 59 individuals who had undergone coronary angiographic evaluation. Of these, 29 were patients diagnosed with CAD (mean age =61.5±10.3) and 30 were controls examined for reasons other than suspected CAD and who had angiographically documented normal coronary arteries (mean age =60.4±11.6). Basal DNA damage as well as pyrimidine and purine base damage were evaluated in peripheral blood lymphocytes using the modified comet assay. Polymerase chain reaction-restriction length polymorphism (PCR-RFLP)-based assay was used for genotyping.Results: Basal DNA damage levels in patients [9.16 (3.26)] were significantly higher than those in controls [7.59 (3.23); p=0.017], and basal DNA and pyrimidine base damage levels were significantly correlated with disease severity based on Gensini scoring (r=0.352, p=0.006; r=0.318, p=0.014, respectively). However, no significant differences were observed in terms of oxidized DNA bases between patients and controls. The frequencies of studied genotypes (GSTM1, GSTT1, and hOGG1) were similar between groups.Conclusion: The results of this study pointed out the role of DNA damage in CAD and its severity. However, GSTM1, GSTT1, and hOGG1 gene polymorphisms seemed to have no effect on individual susceptibility for disease progression
Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA kadioglu e, TAÇOY G, ÖZÇAĞLI E, okyay k, AKBOĞA M, cengel a, ŞARDAŞ S (2016). The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. , 931 - 939.
Chicago kadioglu ela,TAÇOY GÜLTEN,ÖZÇAĞLI Eren,okyay kaan,AKBOĞA Mehmet K,cengel atiye,ŞARDAŞ SEMRA The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. (2016): 931 - 939.
MLA kadioglu ela,TAÇOY GÜLTEN,ÖZÇAĞLI Eren,okyay kaan,AKBOĞA Mehmet K,cengel atiye,ŞARDAŞ SEMRA The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. , 2016, ss.931 - 939.
AMA kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. . 2016; 931 - 939.
Vancouver kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. . 2016; 931 - 939.
IEEE kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S "The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk." , ss.931 - 939, 2016.
ISNAD kadioglu, ela vd. "The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk". (2016), 931-939.
APA kadioglu e, TAÇOY G, ÖZÇAĞLI E, okyay k, AKBOĞA M, cengel a, ŞARDAŞ S (2016). The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. The Anatolian Journal of Cardiology, 16(12), 931 - 939.
Chicago kadioglu ela,TAÇOY GÜLTEN,ÖZÇAĞLI Eren,okyay kaan,AKBOĞA Mehmet K,cengel atiye,ŞARDAŞ SEMRA The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. The Anatolian Journal of Cardiology 16, no.12 (2016): 931 - 939.
MLA kadioglu ela,TAÇOY GÜLTEN,ÖZÇAĞLI Eren,okyay kaan,AKBOĞA Mehmet K,cengel atiye,ŞARDAŞ SEMRA The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. The Anatolian Journal of Cardiology, vol.16, no.12, 2016, ss.931 - 939.
AMA kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. The Anatolian Journal of Cardiology. 2016; 16(12): 931 - 939.
Vancouver kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk. The Anatolian Journal of Cardiology. 2016; 16(12): 931 - 939.
IEEE kadioglu e,TAÇOY G,ÖZÇAĞLI E,okyay k,AKBOĞA M,cengel a,ŞARDAŞ S "The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk." The Anatolian Journal of Cardiology, 16, ss.931 - 939, 2016.
ISNAD kadioglu, ela vd. "The role of oxidative DNA damage and GSTM1, GSTT1, and hOGG1 gene polymorphisms in coronary artery disease risk". The Anatolian Journal of Cardiology 16/12 (2016), 931-939.