Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi

SIRELI, Ufuk Tansel; TERZİ GÜLEL, GÖKNUR; gücükoğlu, ali; YILDIRIM, Yeliz; erdem, murat

Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi

ALİ GÜCÜKOĞLU, (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun-TURKEY)

Yeliz YILDIRIM, (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Erciyes University, Kayseri-TURKEY)

Göknur TERZİ GÜLEL, (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun-TURKEY)

Murat ERDEM, (Department of Chemistry, Faculty of Science, Anadolu University, Eskişehir-TURKEY)

Ufuk Tansel SIRELI (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ankara University Ankara-TURKEY)

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2016 Cilt: 13 Sayı: 1 Sayfa Aralığı: 11 - 18 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi

Öz:

Bu çalışmanın amacı, farklı moleküler ağırlıktaki kitosan solusyonlarının mayonez bazlı salatalardan elde edilen üç Listeria monocytogenes saha izolatı ile L. monocytogenes (ATCC 7644) referans suşu üzerindeki etkilerini in vitro koşullarda değerlendirmekti. İn vitro koşullarda bütün L. monocytogenes izolatları sayısal olarak 2.0x105 cfu/mL'ye ayarlandıktan sonra farklı moleküler ağırlıktaki (düşük, orta ve yüksek) kitosanların %1'lik asetik asit içersinde çözündürülmek suretiyle farklı pH değerlerine (4.0, 4.5 ve 5.0) sahip % 0.1'lik solusyonları hazırlandı. Her bir izolat hazırlanan kitosan solusyonlarıyla muamele edildi. Düşük moleküler ağırlıklı ve pH değeri 4 olan kitosan solusyonuna maruz bırakılan bütün L. monocytogenes suşlarının 24 saat sonunda 3 log düzeyinde inhibe olduğu, orta ve yüksek moleküler ağırlıklı kitosan solusyonuna maruz bırakılanlarda ise 2 log düzeyinde bir inhibisyon şekillendiği gözlemlendi. Çalışmada in vitro koşullarda farklı moleküler ağırlıklı ve farklı pH değerine sahip kitosan solusyonlarının L. monocytogenes üzerine istatistiksel olarak farklı inhibitorik etkiler ortaya koyduğu belirlendi.

Anahtar Kelime:

Konular: Biyoloji Mühendislik, Kimya

In vitro Effects of Chitosan on the Survival of Listeria monocytogenes

Öz:

The aim of this study is to evaluate the in vitro effects of different molecular weights of chitosan on the growth of three Listeria monocytogenes strains isolated from mayonnaise-based salad and of a L. monocytogenes reference strain (ATCC 7644). All L. monocytogenes isolates were numerically adjusted to 2.0x105 cfu/mL and were treated with 0.1% chitosan solutions that had been prepared by dissolving low, medium and high molecular weight chitosan in 1% acetic acid at different pH values (4.0, 4.5 and 5.0) in vitro. All L. monocytogenes isolates were inhibited 3 log levels following 24 h incubation in a low molecular weight chitosan solution at pH 4.0, whereas 2 log levels of inhibition were observed for medium and high molecular weight chitosan solutions. The effect of different molecular weighted chitosan solutions and pH on L. monocytogenes strains in vitro was found to be statistically significant.

Anahtar Kelime:

Konular: Biyoloji Mühendislik, Kimya

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	gücükoğlu a, YILDIRIM Y, TERZİ GÜLEL G, erdem m, SIRELI U (2016). Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. , 11 - 18.
Chicago	gücükoğlu ali,YILDIRIM Yeliz,TERZİ GÜLEL GÖKNUR,erdem murat,SIRELI Ufuk Tansel Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. (2016): 11 - 18.
MLA	gücükoğlu ali,YILDIRIM Yeliz,TERZİ GÜLEL GÖKNUR,erdem murat,SIRELI Ufuk Tansel Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. , 2016, ss.11 - 18.
AMA	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. . 2016; 11 - 18.
Vancouver	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. . 2016; 11 - 18.
IEEE	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U "Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi." , ss.11 - 18, 2016.
ISNAD	gücükoğlu, ali vd. "Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi". (2016), 11-18.

APA	gücükoğlu a, YILDIRIM Y, TERZİ GÜLEL G, erdem m, SIRELI U (2016). Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 11 - 18.
Chicago	gücükoğlu ali,YILDIRIM Yeliz,TERZİ GÜLEL GÖKNUR,erdem murat,SIRELI Ufuk Tansel Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13, no.1 (2016): 11 - 18.
MLA	gücükoğlu ali,YILDIRIM Yeliz,TERZİ GÜLEL GÖKNUR,erdem murat,SIRELI Ufuk Tansel Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol.13, no.1, 2016, ss.11 - 18.
AMA	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2016; 13(1): 11 - 18.
Vancouver	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2016; 13(1): 11 - 18.
IEEE	gücükoğlu a,YILDIRIM Y,TERZİ GÜLEL G,erdem m,SIRELI U "Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi." Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13, ss.11 - 18, 2016.
ISNAD	gücükoğlu, ali vd. "Kitosan'ın İn vitro Koşullarda Listeria monocytogenes Üzerine Etkisi". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13/1 (2016), 11-18.