Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity

KUFREVIOGLU, Omer Irfan; ÇIFTCI, MEHMET; TEMEL, Yusuf

Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity

YUSUF TEMEL, (Bingöl Üniversitesi Solhan Sağlık Hizmetleri Meslek Yüksekokulu)

ÖMER İRFAN KÜFREVİOĞLU, (Atatürk Üniversitesi, Fen Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

MEHMET ÇİFTCİ (Bingöl Üniversitesi, Fen Edebiyat Fakültesi Kimya Bölümü)

Turkish Journal of Chemistry

1 0

Yıl: 2017 Cilt: 41 Sayı: 1 Sayfa Aralığı: 48 - 60 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity

Öz:

The thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with 3.07 EU × mg −1 specific activity was purified 990-fold in a yield of 2.05% using 2 ,5 -ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be 45.5 kDa by SDSPAGE. The molecular mass of the enzyme s natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography. In addition, characteristic and kinetic properties of the enzyme were determined. Then the inhibitory effects of some heavy metal ions (Ag + , Fe 3+ , Cd 2+ , Cu 2+ , Zn 2+ , Pb 2+ , Ni 2+ , and Co 2+) on the activity of TrxR enzyme were examined under in vitro conditions. IC50 values were found with the heavy metal concentration with which 50% of the activity of the TrxR enzyme was inhibited. Finally, Ki values for these substances were calculated from the Lineweaver Burk plots. It was determined that Ag + , Fe 3+ , Cd 2+ , Cu 2+ , and Zn 2+ ions inhibited TrxR enzyme, Pb 2+ ion increased enzyme activity, and Ni 2+ and Co 2+ ions had no effect on enzyme activity.

Anahtar Kelime:

Konular: Mühendislik, Kimya

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TEMEL Y, KUFREVIOGLU O, ÇIFTCI M (2017). Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. , 48 - 60.
Chicago	TEMEL Yusuf,KUFREVIOGLU Omer Irfan,ÇIFTCI MEHMET Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. (2017): 48 - 60.
MLA	TEMEL Yusuf,KUFREVIOGLU Omer Irfan,ÇIFTCI MEHMET Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. , 2017, ss.48 - 60.
AMA	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. . 2017; 48 - 60.
Vancouver	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. . 2017; 48 - 60.
IEEE	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M "Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity." , ss.48 - 60, 2017.
ISNAD	TEMEL, Yusuf vd. "Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity". (2017), 48-60.

APA	TEMEL Y, KUFREVIOGLU O, ÇIFTCI M (2017). Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry, 41(1), 48 - 60.
Chicago	TEMEL Yusuf,KUFREVIOGLU Omer Irfan,ÇIFTCI MEHMET Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry 41, no.1 (2017): 48 - 60.
MLA	TEMEL Yusuf,KUFREVIOGLU Omer Irfan,ÇIFTCI MEHMET Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry, vol.41, no.1, 2017, ss.48 - 60.
AMA	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry. 2017; 41(1): 48 - 60.
Vancouver	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity. Turkish Journal of Chemistry. 2017; 41(1): 48 - 60.
IEEE	TEMEL Y,KUFREVIOGLU O,ÇIFTCI M "Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity." Turkish Journal of Chemistry, 41, ss.48 - 60, 2017.
ISNAD	TEMEL, Yusuf vd. "Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity". Turkish Journal of Chemistry 41/1 (2017), 48-60.