In this study, biodiesel was produced by turning the raw oil, obtained from safflower seed, into Safflower Oil Methyl Ester by transesterification method. Mixing the biodiesel fuel, which was obtained from the safflower, with diesel fuel by adding bioethanol at the rate of 2.5% and 5% and volumetrically in inverse proportion, test fuels were acquired in the form of D100, E2.5B2.5D95, E5B5D90, E5B2.5D92.5 and E2.5B5D92.5. The tests of the diesel fuel and the obtained fuel blends were carried out related to the fuel features; kinematic viscosity, density, water content, pH, color, calorific value, flash point, cloud, pour and freezing points, copper strip corrosion test, iodine number, cold filter plugging point and cetane number. Also, the obtained blend was tried and examined on a four-cycle, single cylinder and on water cooled diesel engine with direct injection fuel system. The engine ran for 100 hours under partial load and the samples were taken from the engine oil at specific times and wear elements were searched. As a result of the analysis of the samples taken from engine lubricating oil, it was observed that as the engine run time increased, the wear elements in the samples increased, too. As a result of ICP oil analysis, the amount of wear elements such as iron, copper, aluminum, lead and chromium in engine lubricating oil were stated diagrammatically. It was determined that the use of E2.5B5D92.5 produced better results than the other types of fuels in terms of wear elements