Yıl: 2017 Cilt: 41 Sayı: 4 Sayfa Aralığı: 347 - 355 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Assessment of genetic diversity of rose genotypes using ISSR markers

Öz:
The genus Rosa L. (Rosaceae) is an important ornamental crop with a high economic value in floriculture, cosmetics, and pharmaceutical industries. Turkey is an important country in rose cultivation with unique species and cultivars, especially for rose oil production. Breeding programs of different rose genotypes are crucial for the development of new commercial varieties. In this study, genetic relationships among the old rose cultivars locally grown in Isparta were assessed by DNA fingerprinting. Inter-simple sequence repeats markers (ISSRs) were used to investigate the genetic diversity among nineteen locally grown rose genotypes. Nineteen ISSR primers yielded a total of 413 scorable amplified fragments. Nei s genetic identity values ranged from 0.545 to 0.951. Nei s genetic distance was used to construct a dendrogram using the unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis. The dendrogram grouped the nineteen rose genotypes into four distinct clusters, and the principal coordinate analysis revealed similar genetic groups. The results confirmed the usefulness of ISSR markers to assess the genetic diversity among the selected old rose genotypes for genetic conservation and plant improvement.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Ağaoğlu YS, Ergül A, Baydar N (2000). Molecular analysis of genetic diversity oil rose (Rosa damascena Mill.) grown Isparta (Turkey) Region. Biotechnol Biotec Eq 14: 16-18.
  • Arnau G, Lallemand J, Bourgoin M (2002). Fast and reliable strawberry cultivar identification using inter simple sequence repeat (ISSR) amplification. Euphytica 129: 69-79.
  • Aminul Islam AKM, Anuar N, Yaakob Z, Osman M (2016). Genetic diversity among candidate plus plants (CPPs) in jatropha (Jatropha curcas). Pakistan Journal of Agricultural Sciences 53: 585-592.
  • Atienza SG, Torres AM, Millan T, Cubero JI (2005). Genetic diversity in Rosa as revealed by RAPDs. Agriculturae Conspectus Scientificus 70: 75-85.
  • Baser KHC, Kürkçüoğlu M, Özek T (2003). Turkish rose oil research: recent results. Perfumer and Flavorist 28: 34-42.
  • Carvalho A, Lima-Brito J, Macas B, Guedes-Pinto H (2009). Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochem Genet 47: 276-294.
  • Crespel L, Pernet A, Le Bris M, Gudin S, Hibrand Saint Oyant L (2009). Application of ISSRs for cultivar identification and assessment of genetic relationships in rose. Plant Breeding 12: 501-506.
  • De RN, Reddy JN, Rao AVS, Mohant KK (1992). Genetic divergence in early rice under two situations. Indian J Genet Pl Br 52: 225- 229.
  • Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
  • Gault SM, Synge PM (1971). Introduction. In: The Dictionary of Roses in Color. Hague, the Netherlands: Xi Ebury Press and Medical Joseph, p. 11.
  • Golkar P, Arzani A, Rezaei AM (2011). Genetic variation in safflower (Carthamus tinctorious L.) for seed quality-related traits and intersimple sequence repeat (ISSR) markers. Int J Mol Sci 12: 2664-2677.
  • Gudin S (2000). Rose: genetics and breeding. In: Janick J, editor. Plant Breeding Reviews. New York, NY, USA: John Wiley and Sons, Inc., pp. 159-189.
  • Gupta M, Chyi YS, Romero-Severson J, Owen JL (1994). Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor Appl Genet 89: 998- 1006.
  • Jaccard P (1980). Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223-270 (in French).
  • Joshi SP, Gupta YS, Aggarwal RK, Ranjekar PK, Brar DS (2000). Genetic diversity and phylogenetic relationship as revealed by inter-simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor Appl Genet 100: 1311-1320.
  • Machkour-M’Rabet S, Hénaut Y, Dor A, Pérez-Lachaud G, Pélissier C, Legal L (2009). ISSR (inter simple sequence repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorpae). J Arachnol 37: 10-14.
  • Mezghani N, Debbabi OS, Rouz S, Medini M (2015). AFLP markers for the assessment of genetic variability in rose (Rosa gallica L.) cultivars in Tunisia. International Journal of Advanced Research 3: 35-42.
  • Nadeem M, Wang X, Akond M, Awan FS, Riaz A, Younis A (2014). Hybrid identification, morphological evaluation and genetic diversity analysis of Rosa x hybrida by SSR markers. Australian Journal of Crop Science 8: 183-190.
  • Najaphy A, Parchin RA, Farshadfar E (2012). Comparison of phenotypic and molecular characterizations of some important wheat cultivars and advanced breeding lines. Australian Journal of Crop Science 6: 326-332.
  • Nei M (1972). Genetic distance between populations. Am Nat 106: 283-292.
  • Nilsson O (1972). Rosa. In: Davis PH, editor. Flora of Turkey and the East Aegean Islands, Vol. 4. Edinburgh, UK: Edinburgh University Press, pp. 106-128.
  • Özçelik H (2010). New records and systematical contributions to garden roses of Türkiye. The Herb Journal of Systematic Botany 17: 9-42.
  • Özçelik H, Özgökçe F, Ünal M, Korkmaz M (2012). The diversity centers and ecological characteristics of Rosa L. (Rosaceae) taxa in Türkiye. International Research Journal of Plant Science 3: 230-237.
  • Panwar S, Singh K, Sonah KP, Deshmukh RK, Namita B, Prasad KV, Sharma T (2015). Molecular fingerprinting and assessment of genetic diversity in rose (Rosa × hybrida). Indian J Biotechnol 14: 518-524.
  • Rajapakse S, Zhang L, Ballard RE, Byrne DH (2001). AFLP marker development in rose for genetic mapping: comparison of three restriction enzyme pairs. Acta Hortic 546: 619-627.
  • Qiu XQ, Zhang H, Wang QG, Jian HY, Yan HJ, Zhang T, Wang JH, Tang, KX (2012). Phylogenetic relationships of wild roses in China based on nrDNA and matK data. Sci Hortic-Amsterdam 140: 45-51.
  • Rohlf FJ (1998). NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.00. Setauket, NY, USA: Exeter Software.
  • Schum A, Hoffman K (2001). Use of isolated protoplasts in rose breeding. Acta Hortic 547: 35-45.
  • Singh SP (1991). Genetic divergence and canonical analysis in hyacinth bean (Dolichos lablab). J Genet Plant Breed 45: 7-12.
  • Singh SP, Shukla S (1998). Genetic divergence in relation to breeding for fatty acids in opium poppy (Papaver somniferum L.). Journal of Genetics and Breeding 52: 301-306.
  • Virk PS, Zhu I, Newbury HJ, Bryan GJ, Jackson MT, Ford-Lloyd BY (2000). Effectiveness of different classes of molecular marker for classifying and revealing variation in rice (Oryza sativa) germplasm. Euphytica 112: 275-284.
  • Xu Z, Zhao L, Zhang L, Yang Z (2011). Analysis of genetic diversity and construction of fingerprint of Rosa rugosa by SRAP. Scientia Agricultura Sinica 44: 1662-1669.
  • Yang SH, Guo N (2015). AFLP-based genetic diversity of Rosa beggeriana populations in Tianshan mountains of Xinjiang. Acta Horticulturae Sinica 42: 1823-1830.
  • Yang SH, Guo N, Ge WY, Ge H (2013). AFLP-based genetic diversity among the populations of Rosa laxa in Tianshan mountains of Xinjiang. China Acta Horticulturae 977: 307-312.
  • Zeinali H, Tabaei-Aghdaei SR, Arzani A (2009). A study of morphological variations and their relationship with flower yield and yield components in Rosa damascena. J Agric Sci Technol 11: 439-448.
  • Zhang LH, Byrne DH, Ballard RE, Rajapakse S (2006). Microsatellite marker development in rose and its application in tetraploid mapping. J Am Soc Hortic Sci 131: 380-387.
  • Zietkiewicz E, Rafalski A, Labuda D (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
APA OĞRAŞ TALAS T, KOBAN BAŞTANLAR E, KARAKAŞ METİN Ö, KANDEMİR İ, ÖZÇELİK H (2017). Assessment of genetic diversity of rose genotypes using ISSR markers. , 347 - 355.
Chicago OĞRAŞ TALAS Tijen,KOBAN BAŞTANLAR Evren,KARAKAŞ METİN ÖZGE,KANDEMİR İRFAN,ÖZÇELİK Hasan Assessment of genetic diversity of rose genotypes using ISSR markers. (2017): 347 - 355.
MLA OĞRAŞ TALAS Tijen,KOBAN BAŞTANLAR Evren,KARAKAŞ METİN ÖZGE,KANDEMİR İRFAN,ÖZÇELİK Hasan Assessment of genetic diversity of rose genotypes using ISSR markers. , 2017, ss.347 - 355.
AMA OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H Assessment of genetic diversity of rose genotypes using ISSR markers. . 2017; 347 - 355.
Vancouver OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H Assessment of genetic diversity of rose genotypes using ISSR markers. . 2017; 347 - 355.
IEEE OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H "Assessment of genetic diversity of rose genotypes using ISSR markers." , ss.347 - 355, 2017.
ISNAD OĞRAŞ TALAS, Tijen vd. "Assessment of genetic diversity of rose genotypes using ISSR markers". (2017), 347-355.
APA OĞRAŞ TALAS T, KOBAN BAŞTANLAR E, KARAKAŞ METİN Ö, KANDEMİR İ, ÖZÇELİK H (2017). Assessment of genetic diversity of rose genotypes using ISSR markers. Turkish Journal of Botany, 41(4), 347 - 355.
Chicago OĞRAŞ TALAS Tijen,KOBAN BAŞTANLAR Evren,KARAKAŞ METİN ÖZGE,KANDEMİR İRFAN,ÖZÇELİK Hasan Assessment of genetic diversity of rose genotypes using ISSR markers. Turkish Journal of Botany 41, no.4 (2017): 347 - 355.
MLA OĞRAŞ TALAS Tijen,KOBAN BAŞTANLAR Evren,KARAKAŞ METİN ÖZGE,KANDEMİR İRFAN,ÖZÇELİK Hasan Assessment of genetic diversity of rose genotypes using ISSR markers. Turkish Journal of Botany, vol.41, no.4, 2017, ss.347 - 355.
AMA OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H Assessment of genetic diversity of rose genotypes using ISSR markers. Turkish Journal of Botany. 2017; 41(4): 347 - 355.
Vancouver OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H Assessment of genetic diversity of rose genotypes using ISSR markers. Turkish Journal of Botany. 2017; 41(4): 347 - 355.
IEEE OĞRAŞ TALAS T,KOBAN BAŞTANLAR E,KARAKAŞ METİN Ö,KANDEMİR İ,ÖZÇELİK H "Assessment of genetic diversity of rose genotypes using ISSR markers." Turkish Journal of Botany, 41, ss.347 - 355, 2017.
ISNAD OĞRAŞ TALAS, Tijen vd. "Assessment of genetic diversity of rose genotypes using ISSR markers". Turkish Journal of Botany 41/4 (2017), 347-355.