Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

DİLİK, Zahide; ÜNAL, NİLGÜN; YURTERİ, Buket; AŞKAR, Şinasi; KARAGOZ, ALPER

Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

Nilgün ÜNAL, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Kırıkkale, Türkiye)

Alper KARAGÖZ, (Türkiye Halk Sağlığı Enstitüsü, Mikrobiyoloji Referans Laboratuvarları Bölümü, Ankara, Türkiye)

Şinasi AŞKAR, (Çankırı Karatekin Üniversitesi, Sağlık Yüksekokulu, Beslenme ve Diyetetik Bölümü, Çankırı, Türkiye)

Zahide DİLİK, (Bornova Veteriner Kontrol ve Araştırma Enstitüsü, İzmir, Türkiye)

Buket YURTERİ (Sağlık Bakanlığı, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Dışkapı, Ankara, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2017 Cilt: 41 Sayı: 1 Sayfa Aralığı: 72 - 76 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey

Öz:

The aim of this study was to determine the prevalence and clonal typing of extended-spectrum beta-lactamase (ESBL)- producingEscherichia coliin healthy broilers in Turkey. Three hundred broiler cloacal samples were collected from various broilerslaughterhouses and inoculated on Levine agar plates supplemented with 2 μg/mL cefotaxime. Suspected strains were identified usinga BBL Crystal Enteric/Nonfermenter ID Kit (Becton Dickinson, USA) and ESBL production was confirmed using an ESBL phenotypicconfirmatory test. ESBL types were analyzed using PCR and sequencing. Pulsed field gel electrophoresis (PFGE) was performed withXbaI for the clonal typing of ESBL-producingE. coliisolates. While 33 phenotypic ESBL-producingE. coliisolates were identified, eightof them had only the blaTEM-1. Twenty-five ESBL-producing isolates were detected. This research is the first on the investigation anddetection of ESBL-producingE. coliisolates from broilers in Turkey. In this study, 8.3% ESBL-producingE. coliwere isolated from thecloacal samples of broilers collected from slaughterhouses in Turkey. CTX-M-15 (80%) was the most frequently isolated ESBL type.Using PFGE analysis, it was determined that these isolates had clonal similarity.

Anahtar Kelime:

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Smet A, Martel A, Persoons D, Dewulf J, Heyndrickx M, Catry B, Herman L, Haesebrouck F, Butaye P. Diversity of extended- spectrum β-lactamases and class C β-lactamases among cloacal Escherichia coli isolates in Belgian broiler farms. Vet Mic 2008; 144: 505-510.
2. Olsen RH, Bisgaard M, Löhren U, Robineau B, Christensen H. Extended-spectrum β-lactamase-producing Escherichia coli isolated from poultry: a review of current problems, illustrated with some laboratory findings. Avian Pathol 2014; 43: 199-208.
3. Ewers C, Antao EM, Diehl I, Philipp HC, Wieler LH. Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Appl Environ Microbiol 2009; 75: 184-192.
4. Li L, Jiang ZG, Xia LN, Shen JZ, Dai L, Wang Y, Huang SY, Wu CM. Characterization of antimicrobial resistance and molecular determinants of beta-lactamase in Escherichia coli isolated from chickens in China during 19702007. Vet Microbiol 2010; 26: 505-510.
5. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical Veterinary Microbiology. 2nd ed. Mosby Elsevier, China; 2013.
6. CLSI - Clinical Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Document M100-S23. Twenty-third Informational Supplement. Wayne, PA, USA: 2013.
7. Briñas L, Moreno MA, Zarazaga M, Porrero C, Sáenz Y, García M, Dominguez L, Torres C. Detection of CMY-2, CTX-M-14, and SHV-12 β-lactamases in Escherichia coli fecal-sample isolates from healthy chickens. Antimicrob Agents Chemother 2003; 47: 2056-2058.
8. Jouini A, Vinue L, Slama KB, Saenz Y, Klibi N, Hammami S, Boudabous A, Torres C. Characterization of CTX-M and SHV extended-spectrum β-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia. J Antimicrob Chemother 2007; 60: 1137-1141.
9. Sáenz Y, Briñas L, Domínguez E, Ruiz J, Zarazaga M, Vila J, Torres C. Mechanisms of resistance in multiple-antibiotic- resistant Escherichia coli strains of human, animal and food origins. Antimicrob Agents Chemother 2004; 48: 3996-4001.
10. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-2239.
11. Randall LP, Clouting C, Horton RA, Goldham NG, Wu G, Clifton-Hadley FA, Davies R H, Teale CJ. Prevalence of Escherichia coli carrying extended-spectrum β-lactamases (CTX - M and TEM - 52) from broiler chickens and turkeys in Great Britain between 2006 and 2009. J Antimicrob Chemother 2011; 66: 86-95.
12. Girlich D, Poirel L, Carattoli A, Kempf I, Lartigue MF, Bertini A, Nordmann P. Extended-spectrum beta-lactamase CTX-M-1 in Escherichia coli isolates from healthy poultry in France. Appl Environ Microbiol 2007; 73: 4681-4685.
13. Costa D, Vinue L, Poeta P, Coelho AC, Matos M, Saenz Y, Somaldo S, Zarazaga M, Rodrigues J, Torres C. Prevalence of extended-spectrum beta-lactamase-producing Escherichia coli isolates in fecal samples of broiler. Vet Mic 2009; 138: 339-344.
14. Yuan L, Liu JH, Hu GZ, Pan YS, Liu ZM, Mo J, Wei YJ. Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China. J Med Microbiol 2009; 58: 1449-1453.
15. Dierikx CM, van der Goot JA, Smith HE, Kant A, Mevius DJ. Presence of ESBL/AmpC-producing Escherichia coli in the broiler production pyramid: a descriptive study. PLoS ONE 2013; 8: e79005. doi: 10.1371/journal.pone.0079005.
16. Warren RE, Ensor VM, ONeill P, Butler V, Taylor J, Nye K, Harvey M, Livermore DM, Woodford N, Hawkey PM. Imported chicken meat as a potential source of quinolone- resistant Escherichia coli producing extended-spectrum β-lactamases in the UK. Antimicrob Agents Chemother 2008; 61: 504-508.
17. Pitout JDD, Nordmann P, Laupland KB, Poirel L. Emergence of Enterobacteriacea producing extended-spectrum β-lactamases (ESBLs) in the community. J Antimicrob Chemother 2005; 56: 52-59.
18. Bortolaia V, Guardabassi L, Trevisani M, Bisgaard M, Venturi L, Bojesen AM. High diversity of extended-spectrum β-lactamases in Escherichia coli isolates from Italian broiler flocks. Antimicicrob Agents Chemother 2010; 54: 1623-1626.
19. Tong P, Sun Y, Ji X, Du X, Guo X, Liu J, Zhu L, Zhou B, Zhou W, Liu G et al. Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in Escherichia coli isolated from chickens. Foodborne Pathog Dis 2015; 12: 345- 352.
20. Maciuca IE, Williams NJ, Tuchilus C, Dorneanu O, Guguianu E, Carp-Carare C, Rimbu C, Timofte D. High prevalence of Escherichia coli -producing CTX-M-15 extended-spectrum beta-lactamases in poultry and human clinical isolates in Romania. Microb Drug Resist. 2015; 21: 651-662.
21. Gür D, Gülay Z, Arıkan Akan Ö, Aktaş Z, Bal Kayacan Ç, Çakıcı Ö, Eraç B, Gültekin M, Öğünç D, Söyletir G et al. Resistance to newer beta-lactams and related ESBL types in Gram-negative nosocomial isolates in Turkish hospitals: results of the multicentre Hitit study. Mikrobiyol Bul 2008; 42: 537-544.

APA	ÜNAL N, KARAGOZ A, AŞKAR Ş, DİLİK Z, YURTERİ B (2017). Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. , 72 - 76.
Chicago	ÜNAL NİLGÜN,KARAGOZ ALPER,AŞKAR Şinasi,DİLİK Zahide,YURTERİ Buket Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. (2017): 72 - 76.
MLA	ÜNAL NİLGÜN,KARAGOZ ALPER,AŞKAR Şinasi,DİLİK Zahide,YURTERİ Buket Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. , 2017, ss.72 - 76.
AMA	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. . 2017; 72 - 76.
Vancouver	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. . 2017; 72 - 76.
IEEE	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B "Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey." , ss.72 - 76, 2017.
ISNAD	ÜNAL, NİLGÜN vd. "Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey". (2017), 72-76.

APA	ÜNAL N, KARAGOZ A, AŞKAR Ş, DİLİK Z, YURTERİ B (2017). Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. Turkish Journal of Veterinary and Animal Sciences, 41(1), 72 - 76.
Chicago	ÜNAL NİLGÜN,KARAGOZ ALPER,AŞKAR Şinasi,DİLİK Zahide,YURTERİ Buket Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. Turkish Journal of Veterinary and Animal Sciences 41, no.1 (2017): 72 - 76.
MLA	ÜNAL NİLGÜN,KARAGOZ ALPER,AŞKAR Şinasi,DİLİK Zahide,YURTERİ Buket Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.41, no.1, 2017, ss.72 - 76.
AMA	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. Turkish Journal of Veterinary and Animal Sciences. 2017; 41(1): 72 - 76.
Vancouver	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey. Turkish Journal of Veterinary and Animal Sciences. 2017; 41(1): 72 - 76.
IEEE	ÜNAL N,KARAGOZ A,AŞKAR Ş,DİLİK Z,YURTERİ B "Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey." Turkish Journal of Veterinary and Animal Sciences, 41, ss.72 - 76, 2017.
ISNAD	ÜNAL, NİLGÜN vd. "Extended-spectrum β-lactamases among cloacal Escherichia coli isolates in healthy broilers in Turkey". Turkish Journal of Veterinary and Animal Sciences 41/1 (2017), 72-76.