Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells

uslu, serap; ŞAHİN, HASAN HÜSEYİN; Yegen, Berrak; KURTEL, HIZIR; Alaca, Nuray; Özbeyli, Dilek; Yigitturk, Gurkan; Oktem, Gulperi

Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells

Nuray ALACA, (Department of Physiotherapy and Rehabilitation, Acıbadem University Faculty of Health Sciences, İstanbul, Turkey)

Dilek ÖZBEYLİ, (Department of Physiology, Marmara University School of Medicine, İstanbul, Turkey)

Serap USLU, (Department of Histology and Embryology, İstanbul Medeniyet University School of Medicine, İstanbul, Turkey.)

Hasan Hüseyin ŞAHİN, (Department of Physiology, Marmara University School of Medicine, İstanbul, Turkey)

Gürkan YİĞİTTÜRK, (Department of Histology and Embryology, Ege University School of Medicine, İzmir, Turkey)

Hızır KURTEL, (Department of Physiology, Marmara University School of Medicine, İstanbul, Turkey)

Gülperi ÖKTEM, (Department of Histology and Embryology, Ege University School of Medicine, İzmir, Turkey)

Berrak Çağlayan YEĞEN (Department of Physiology, Marmara University School of Medicine, İstanbul, Turkey)

Turkish Journal of Gastroenterology

18 0

Yıl: 2017 Cilt: 28 Sayı: 6 Sayfa Aralığı: 476 - 484 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells

Öz:

Background/Aims: Cholestasis, which results in hepatic cell death, fibrosis, cirrhosis, and eventually liver failure, is associated with oxidative stress. The aim of this study was to evaluate the effects of milk thistle (MT, Silybum marianum) and ursodeoxycholic acid (UDCA) or their combination on the activation of hepatic stem cells and on the severity of cholestasis liver injury in rats. Materials and Methods: Under anesthesia, bile ducts of female Sprague Dawley rats were ligated (BDL) or had sham operation. BDL rats were administered saline, UDCA (15 mg/kg/d), MT (600 mg/kg/d), or UDCA+MT by gavage for 10 days. On the 11th day, rats were sacrificed and blood and liver samples were obtained. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic malondialdehyde (MDA) levels, and myeloperoxidase (MPO) activity were measured. Hepatic injury, a-smooth muscle actin expression, and stem cell markers c-kit, c-Myc, Oct3/4, and SSEA-1 were histologically determined. Results: Histological scores, serum ALT, and hepatic MDA levels were higher in BDL group than in the sham rats, while all treatments significantly reduced these levels. The reduction in ALT was significantly greater in UCDA+MT-treated group than in other treatment groups. c-Kit, c-Myc, Oct3/4, and SSEA-1 were increased in saline-treated BDL group with respect to sham-operated control group, and these markers were significantly reduced in all treatment groups. Conclusion: In addition to a modulatory effect on the stem cell-induced regenerative response of the liver, UDCA, MT, and their combination demonstrated similar anti-inflammatory and antiproliferative effects on cholestasis-induced hepatic injury

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Alaca N, Özbeyli D, uslu s, ŞAHİN H, Yigitturk G, KURTEL H, Oktem G, Yegen B (2017). Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. , 476 - 484.
Chicago	Alaca Nuray,Özbeyli Dilek,uslu serap,ŞAHİN HASAN HÜSEYİN,Yigitturk Gurkan,KURTEL HIZIR,Oktem Gulperi,Yegen Berrak Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. (2017): 476 - 484.
MLA	Alaca Nuray,Özbeyli Dilek,uslu serap,ŞAHİN HASAN HÜSEYİN,Yigitturk Gurkan,KURTEL HIZIR,Oktem Gulperi,Yegen Berrak Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. , 2017, ss.476 - 484.
AMA	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. . 2017; 476 - 484.
Vancouver	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. . 2017; 476 - 484.
IEEE	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B "Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells." , ss.476 - 484, 2017.
ISNAD	Alaca, Nuray vd. "Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells". (2017), 476-484.

APA	Alaca N, Özbeyli D, uslu s, ŞAHİN H, Yigitturk G, KURTEL H, Oktem G, Yegen B (2017). Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. Turkish Journal of Gastroenterology, 28(6), 476 - 484.
Chicago	Alaca Nuray,Özbeyli Dilek,uslu serap,ŞAHİN HASAN HÜSEYİN,Yigitturk Gurkan,KURTEL HIZIR,Oktem Gulperi,Yegen Berrak Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. Turkish Journal of Gastroenterology 28, no.6 (2017): 476 - 484.
MLA	Alaca Nuray,Özbeyli Dilek,uslu serap,ŞAHİN HASAN HÜSEYİN,Yigitturk Gurkan,KURTEL HIZIR,Oktem Gulperi,Yegen Berrak Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. Turkish Journal of Gastroenterology, vol.28, no.6, 2017, ss.476 - 484.
AMA	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. Turkish Journal of Gastroenterology. 2017; 28(6): 476 - 484.
Vancouver	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells. Turkish Journal of Gastroenterology. 2017; 28(6): 476 - 484.
IEEE	Alaca N,Özbeyli D,uslu s,ŞAHİN H,Yigitturk G,KURTEL H,Oktem G,Yegen B "Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells." Turkish Journal of Gastroenterology, 28, ss.476 - 484, 2017.
ISNAD	Alaca, Nuray vd. "Treatment with milk thistle extract (Silybum marianum), ursodeoxycholic acid, or their combination attenuates cholestatic liver injury in rats: Role of the hepatic stem cells". Turkish Journal of Gastroenterology 28/6 (2017), 476-484.