Yıl: 2017 Cilt: 41 Sayı: 5 Sayfa Aralığı: 841 - 847 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats

Öz:
Wound healing is a complex process affected by various conditions, including oxidative stress. The present study exploredthe time-dependent effects of platelet-derived growth factor (PGDF) and vascular endothelial growth factor (VEGF) administration onoxidative markers during acute stage wound healing. Thirty-six Wistar rats were distributed into three major groups; skin wounds wereinflicted in all groups. The wounds were either left untreated (control group), treated topically with blank chitosan gel (blank chitosangel group), or treated topically with a combination of PDGF and VEGF in chitosan gel (PDGF + VEGF chitosan gel group). Woundswere sampled on postsurgery days 3 and 7; samples were assayed for the oxidant markers nitric oxide (NOx) and thiobarbituric acid- reactive substances (TBARs) and the antioxidant markers glutathione (GSH), ascorbic acid (AA), and superoxide dismutase (SOD)activity. PDGF + VEGF administration increased and decreased NOx levels on days 3 and 7, respectively. PDGF + VEGF administrationlowered TBARs levels, compared with blank chitosan gel administration, on day 7. PDGF + VEGF administration increased GSH levels.These results demonstrate that PDGF + VEGF administration changes oxidative status of wound tissue. This study provides valuableinsights for the development of therapeutic targets that promote wound healing.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Alemdaroglu C, Degim Z, Celebi N, Zor F, Ozturk S, Erdogan D (2006). An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns 32: 319-327.
  • Aykac G, Uysal M, Yalcin AS, Kocak-Toker N, Sivas A, Oz H (1985). The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology 36: 71-76.
  • Barleon B, Sozzani S, Zhou D, Weich HA, Mantovani A, Marmé D (1996). Migration of human monocytes in response to vascular endothelial growth factor (VEGF) is mediated via the VEGF receptor flt-1. Blood 87: 3336-3343.
  • Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008). Growth factors and cytokines in wound healing. Wound Repair Regen 16: 585-601.
  • Berger J, Shepard D, Morrow F, Taylor A (1989). Relationship between dietary intake and tissue levels of reduced and total vitamin C in the nonscorbutic guinea pig. J Nutr 119: 734-740.
  • Breitbart AS, Laser J, Parrett B, Porti D, Grant RT, Grande DA, Mason JM (2003). Accelerated diabetic wound healing using cultured dermal fibroblasts retrovirally transduced with the platelet- derived growth factor B gene. Ann Plast Surg 51: 409-414.
  • Casini AF, Ferrali M, Pompella A, Maellaro E, Comporti M (1986). Lipid peroxidation and cellular damage in extrahepatic tissues of bromobenzene-intoxicated mice. Am J Pathol 123: 520-531.
  • Chen QR, Miyaura C, Higashi S, Murakami M, Kudo I, Saito S, Hiraide T, Shibasaki Y, Suda T (1997). Activation of cytosolic phospholipase A2 by platelet-derived growth factor is essential for cyclooxygenase-2-dependent prostaglandin E2 synthesis in mouse osteoblasts cultured with interleukin-1. J Biol Chem 272: 5952-5958.
  • Chen S, Tsai M, Huang J, Chen R (2009). In vitro antioxidant activities of low-molecular-weight polysaccharides with various functional groups. J Agric Food Chem 57: 2699-2704.
  • Cheng B, Liu HW, Fu XB, Sun TZ, Sheng ZY (2007). Recombinant human platelet-derived growth factor enhanced dermal wound healing by a pathway involving ERK and c-fos in diabetic rats. J Dermatol Sci 45: 193-201.
  • Coskun S, Peker EGG, Balabanlı B, Ahıska S, Acarturk F (2009). Effect of transforming growth factor beta 1 (TGF-beta 1) on nitric oxide production and lipid peroxidation in oral mucosal wound healing. Med Chem Res 20: 23-28.
  • Duffy AM, Bouchier-Hayes DJ, Harmey JH (2004). Vascular endothelial growth factor (VEGF) and its role in non- endothelial cells: autocrine signalling by VEGF. In: Harmey JH, editor. VEGF and Cancer. Austin, TX, USA: Landes Bioscience, pp. 133-144.
  • Esplugues JV (2002). NO as a signalling molecule in the nervous system. Br J Pharmacol 135: 1079-1095.
  • Frank S, Kampfer H, Wetzler C, Pfeilschifter J (2002). Nitric oxide drives skin repair: novel functions of an established mediator. Kidney Int 61: 882-888.
  • Greaves NS, Ashcroft KJ, Baguneid M, Bayat A (2013). Current understanding of molecular and cellular mechanisms in fibroplasia and angiogenesis during acute wound healing. J Dermatol Sci 72: 206-217.
  • Guo S, Dipietro LA (2010). Factors affecting wound healing. J Dent Res 89: 219-229.
  • Harvey R, Ferrier D (2010). Lipid metabolism. In: Harvey R, editor. Lippincott’s Illustrated Reviews: Biochemistry. 5th ed. Philadelphia, PA, USA: Lippincott Williams & Wilkins, pp. 173-244.
  • Heldin CH (2012). Autocrine PDGF stimulation in malignancies. Ups J Med Sci 117: 83-91.
  • Hishikawa K, Nakaki T, Hirahashi J, Marumo T, Saruta T (1996). Tranilast restores cytokine-induced nitric oxide production against platelet-derived growth factor in vascular smooth muscle cells. J Cardiovasc Pharmacol 28: 200-207.
  • Hou YC, Janczuk A, Wang PG (1999). Current trends in the development of nitric oxide donors. Curr Pharm Des 5: 417- 441.
  • Hwang SM, Chen CY, Chen SS, Chen JC (2000). Chitinous materials inhibit nitric oxide production by activated RAW 264.7 macrophages. Biochem Biophys Res Commun 271: 229-233.
  • Iantomasi T, Favilli F, Degl’Innocenti D, Vincenzini MT (1999). Increased glutathione synthesis associated with platelet- derived growth factor stimulation of NIH3T3 fibroblasts. Biochim Biophys Acta 1452: 303-312.
  • Ishihara M, Nakanishi K, Ono K, Sato M, Kikuchi M, Saito Y, Yura H, Matsui T, Hattori H, Uenoyama M et al. (2002). Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials 23: 833-840.
  • Kalay Z, Cevher SC (2012). Oxidant and antioxidant events during epidermal growth factor therapy to cutaneous wound healing in rats. Int Wound J 9: 362-371.
  • Kaltalioglu K, Coskun-Cevher S (2015). A bioactive molecule in a complex wound healing process: platelet-derived growth factor. Int J Dermatol 54: 972-977.
  • Kaltalioglu K, Coskun-Cevher S, Tugcu-Demiroz F, Celebi N (2013). PDGF supplementation alters oxidative events in wound healing process: a time course study. Arch Dermatol Res 305: 415-422.
  • Kirchner LM, Meerbaum SO, Gruber BS, Knoll AK, Bulgrin J, Taylor RAJ, Schmidt SP (2003). Effects of vascular endothelial growth factor on wound closure rates in the genetically diabetic mouse model. Wound Repair Regen 11: 127-131.
  • Koai E, Rincon Rios T, Edwards J (2010). Vascular endothelial growth factor increases endothelial nitric oxide synthase transcription in huvec cells. WebmedCentral Physiology 1: WMC001111.
  • Kroll J, Waltenberger J (1998). VEGF-A induces expression of eNOS and iNOS in endothelial cells via VEGF receptor-2 (KDR). Biochem Biophys Res Commun 252: 743-746.
  • Kunz D, Walker G, Eberhardt W, Messmer UK, Huwiler A, Pfeilschifter J (1997). Platelet-derived growth factor and fibroblast growth factor differentially regulate interleukin 1beta- and cAMP-induced nitric oxide synthase expression in rat renal mesangial cells. J Clin Invest 100: 2800-2809.
  • Kuzuya M, Ramos MA., Kanda S, Koike T, Asai T, Maeda K, Shitara K, Shibuya M, Iguchi A (2001). VEGF protects against oxidized LDL toxicity to endothelial cells by an intracellular glutathione- dependent mechanism through the KDR receptor. Arterioscler Thromb Vasc Biol 21: 765-770.
  • Lauro S, Onesti CE, Righini R, Marchetti P (2014). The use of bevacizumab in non-small cell lung cancer: an update. Anticancer Res 34: 1537-1545.
  • Lee YS, Wysocki A, Warburton D, Tuan TL (2012). Wound healing in development. Birth Defects Res C Embryo Today 96: 213-222.
  • Li WW, Talcott KE, Zhai AW, Kruger EA, Li VW (2005). The role of therapeutic angiogenesis in tissue repair and regeneration. Adv Skin Wound Care 18: 491-500; quiz 501-502.
  • Li H, Fu X, Zhang L, Huang Q, Wu Z, Sun T (2008). Research of PDGF-BB gel on the wound healing of diabetic rats and its pharmacodynamics. J Surg Res 145: 41-48.
  • Lobo V, Patil A, Phatak A, Chandra N (2010). Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4: 118-126.
  • Lushchak VI (2012). Glutathione homeostasis and functions: potential targets for medical interventions. J Amino Acids 2012: ID736837.
  • Miranda KM, Espey MG, Wink DA (2001). A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5: 62-71.
  • Papapetropoulos A, García-Cardeña G, Madri J, Sessa W (1997). Nitric oxide production contributes to the angiogenic properties of vascular endothelial growth factor in human endothelial cells. J Clin Invest 100: 3131-3139.
  • Park JS, Hong GR, Baek SW, Shin DG, Kim YJ, Shim BS (2002). Expression and regulation of endothelial nitric oxide synthase by vascular endothelial growth factor in ECV 304 cells. J Korean Med Sci 17: 161-167.
  • Pattani A, Patravale VB, Panicker L, Potdar PD (2009). Immunological effects and membrane interactions of chitosan nanoparticles. Mol Pharm 6: 345-352.
  • Pierce GF, Tarpley JE, Yanagihara D, Mustoe TA, Fox GM, Thomason A (1992). Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair. Am J Pathol 140: 1375-1388.
  • Przybylski M (2009). A review of the current research on the role of bFGF and VEGF in angiogenesis. J Wound Care 18: 516-519.
  • Rodriguez PG, Felix FN, Woodley DT, Shim EK (2008). The role of oxygen in wound healing: a review of the literature. Dermatol Surg 34: 1159-1169.
  • Sawano A, Iwai S, Sakurai Y, Ito M, Shitara K, Nakahata T, Shibuya M (2001). Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte- macrophages in humans. Blood 97: 785-791.
  • Schwentker A, Vodovotz Y, Weller R, Billiar TR (2002). Nitric oxide and wound repair: role of cytokines? Nitric Oxide 7: 1-10.
  • Scott-Burden T, Schini VB, Elizondo E, Junquero DC, Vanhoutte PM (1992). Platelet-derived growth factor suppresses and fibroblast growth factor enhances cytokine-induced production of nitric oxide by cultured smooth muscle cells. Effects on cell proliferation. Circ Res 71: 1088-1100.
  • Sen CK (2003). The general case for redox control of wound repair. Wound Repair Regen 11: 431-438.
  • Shen H, Clauss M, Ryan J, Schmidt AM, Tijburg P, Borden L, Connolly D, Stern D, Kao J (1993). Characterization of vascular permeability factor/vascular endothelial growth factor receptors on mononuclear phagocytes. Blood 81: 2767-2773.
  • Steed DL (2006). Clinical evaluation of recombinant human platelet- derived growth factor for the treatment of lower extremity ulcers. Plast Reconstr Surg 117: 143S-149S; discussion 150S-151S.
  • Sun Y, Oberley LW, Li Y (1988). A simple method for clinical assay of superoxide dismutase. Clin Chem 34: 497-500.
  • Sundaresan M, Yu ZX, Ferrans VJ, Irani K, Finkel T (1995). Requirement for generation of H 2 O 2 for platelet-derived growth factor signal transduction. Science 270: 296-299.
  • Takehara K (2000). Growth regulation of skin fibroblasts. J Dermatol Sci 24 Suppl 1: S70-77.
  • Yu Z, Zhao L, Ke H (2004). Potential role of nuclear factor-kappaB in the induction of nitric oxide and tumor necrosis factor-alpha by oligochitosan in macrophages. Int Immunopharmacol 4: 193-200.
APA DİNDAR B, Kaltalioglu K, COŞKUN CEVHER Ş (2017). Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. , 841 - 847.
Chicago DİNDAR Bahar,Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. (2017): 841 - 847.
MLA DİNDAR Bahar,Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. , 2017, ss.841 - 847.
AMA DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. . 2017; 841 - 847.
Vancouver DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. . 2017; 841 - 847.
IEEE DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş "Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats." , ss.841 - 847, 2017.
ISNAD DİNDAR, Bahar vd. "Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats". (2017), 841-847.
APA DİNDAR B, Kaltalioglu K, COŞKUN CEVHER Ş (2017). Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. Turkish Journal of Zoology, 41(5), 841 - 847.
Chicago DİNDAR Bahar,Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. Turkish Journal of Zoology 41, no.5 (2017): 841 - 847.
MLA DİNDAR Bahar,Kaltalioglu Kaan,COŞKUN CEVHER ŞULE Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. Turkish Journal of Zoology, vol.41, no.5, 2017, ss.841 - 847.
AMA DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. Turkish Journal of Zoology. 2017; 41(5): 841 - 847.
Vancouver DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats. Turkish Journal of Zoology. 2017; 41(5): 841 - 847.
IEEE DİNDAR B,Kaltalioglu K,COŞKUN CEVHER Ş "Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats." Turkish Journal of Zoology, 41, ss.841 - 847, 2017.
ISNAD DİNDAR, Bahar vd. "Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats". Turkish Journal of Zoology 41/5 (2017), 841-847.