Yıl: 2016 Cilt: 46 Sayı: 3 Sayfa Aralığı: 673 - 679 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients

Öz:
Background/aim: The purpose of the present study was to investigate whether endothelial nitric oxide synthase (eNOS) gene polymorphisms play a role in prostate cancer (PCa). Materials and methods: We examined three eNOS gene polymorphisms (T-786C promoter region, G894T, and Intron 4 VNTR 4a/b) at extracted DNAs from 50 formalin-fixed paraffin-embedded tissues of PCa patients. For the controls, blood samples obtained from 50 healthy men were studied. Genotyping of molecular variants was performed by PCR-RFLP technique. Results: We found that the TC genotype of the T-786C polymorphism was associated with PCa risk (OR: 3.325, CI: 1.350 8.188, P = 0.008). The eNOS G894T polymorphism was also associated with PCa. The frequency of the 894T allele was significantly higher in PCa patients. No association was identified between intron 4 VNTR polymorphism and PCa. Conclusion: We found significant differences in genotypic and allelic frequencies between PCa patients and controls for eNOS T-786C and G894T polymorphisms. The presence of the T-786C genotype and 894T allele in carriers increased the risk of PCa. No association was found between intron 4 VNTR polymorphism and PCa patients.
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Konular: Cerrahi
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Gökalp P, Cücer N, Tatlışen A, İmamoğlu N. Prostat hiperplazi ve kanser doku örneklerinde iki boyutlu AgNOR değişiklerinin karşilaştirilmasi. Sağlık Bilimleri Dergisi 2006; 15: 79-84 (article in Turkish).
  • 2. Kumar V, Cotran R, Robbins S. Temel Patoloji (Çeviri). Çev. ed. Çevikbaş U. İstanbul, Turkey: Nobel Tıp Kitabevi; 2000 (in Turkish).
  • 3. Söylemez B, Özüm Ü. Kanamış kafaiçi anevrizması olan hastalarda endotelyal nitrik oksit sentaz (eNOS) geninin intron 4 (27 baz çifti tekrarı) ve promoter T786C polimorfizmi. Cumhuriyet Med J 2013; 35: 400-406 (in Turkish).
  • 4. Safarinejad MR, Safarinejad S, Shafiei N, Safarinejad S. Effects of the T-786C, G894T, and Intron 4 VNTR (4a/b) polymorphisms of the endothelial nitric oxide synthase gene on the risk of prostate cancer. Urol Oncol 2013; 31: 1132-1140.
  • 5. Ruddon R.W. Cancer Biology. New York, NY, USA: Oxford University press; 1995.
  • 6. Singh D, Febbo PG, Ross K, Jackson DG, Manola J, Ladd C, Tamayo P, Renshaw AA, D’Amico AV, Richie JP et al. Gene expression correlates of clinical prostate cancer behavior. Cancer Cell 2002; 1: 203-209.
  • 7. Glueck, CJ, Munjal, J, Khan, A, Umar, M, Wang, P. Endothelial nitric oxide synthase T-786C mutation, a reversible etiology of Prinzmetal’s Angina Pectoris. Am J Cardiol 2010; 105: 792-796.
  • 8. Sanli O, Kucukgergin C, Gokpinar M, Tefik T, Nane I, Seckin S. Despite the lack of association between different genotypes and the presence of prostate cancer, endothelial nitric oxide synthase a/b (eNOS4a/b) polymorphism may be associated with advanced clinical stage and bone metastasis. Urol Oncol 2011; 29: 183–8.
  • 9. Mattes, WB, Miller, MS. PCR: methods and limitations. In: Miller MS and Cronin MT, editors. Genetic Polymorphisms and Susceptibility to Disease. 1 st ed. London, UK: Taylor and Francis, 2000; pp. 17-39.
  • 10. Thameem F, Puppala S, Arar NH, Stern MP, Blangero J, Duggirala R, Abboud HE. Endothelial nitric oxide synthase (eNOS) gene polymorphisms and their association with type 2 diabetes-related traits in Mexican Americans. Diab Vasc Dis Res 2008; 5: 109-13.
  • 11. Vakkala M, Kahlos K, Lakari E, Paakkö P, KinnulaV, Soini Y. Inducible nitric oxide synthase expression, apoptosis, and angiogenesis in in situ and invasive breast carcinomas. Clin Cancer Res 2000; 6: 2408-2416.
  • 12. Medeiros R, Morais A, Vasconcelos A, Costa S, Pinto D, Oliveira J, Lopes C. Endothelial nitric oxide synthase gene polymorphisms and genetic susceptibility to prostate cancer. Eur J Cancer Prev 2002; 11: 343-350.
  • 13. Marangoni K, Neves AF, Cardoso AM, Santos WK, Faria PC, Goulart LR. The endothelial nitric oxide synthase Glu-298- Asp polymorphism and its mRNA expression in the peripheral blood of patients with prostate cancer and benign prostatic hyperplasia. Cancer Detect Prev 2006; 30: 7-13.
  • 14. Medeiros RM, Morais A, Vasconcelos A, Costa S, Pinto D, Oliveira J, Ferreira P, Lopes C. Outcome in prostate cancer: Association with endothelial nitric oxide synthase Glu-Asp298 polymorphism at exon 7. Clin Cancer Res 2002; 8: 3433-3437.
  • 15. Ziaei SA, Samzadeh M, Jamaldini SH, Afshari M, Haghdoost AA, Hasanzad M. Endothelial nitric oxide synthase Glu298Asp polymorphism as a risk factor for prostate cancer. Int J Biol Marker 2013; 28: 43-48.
  • 16. Asakimori Y, Yorioka N, Taniguchi Y, Ito T, Ogata S, Kyuden Y, Kohno N . T-786C polymorphism of the endothelial nitric oxide synthase gene influences the progression of renal disease. Nephron 2002; 91: 747-751.
  • 17. Nakayama M, Yasue H, Yoshimura M Shimasaki Y, Kugiyama K, Ogawa H, Motoyama T, Saito Y, Ogawa Y, Miyamoto Y, Nakao K. T-786→C mutation in the 5’-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm. Circulation 1999; 99: 2864-2870.
  • 18. Majumdar V, Nagaraja D, Karthik N, Christopher R. Association of Endothelial Nitric Oxide Synthase Gene Polymorphisms with Early-Onset Ischemic Stroke in South Indians. J Atheroscler Thromb 2010; 17: 45-53.
  • 19. Medeiros R, Morais A, Vasconcelos A, Costa S, Carrilho S, Oliveira J, Lopes C. Endothelial nitric oxide synthase gene polymorphisms and the shedding of circulating tumor cells in the blood of prostate cancer patients. Cancer Lett 2003; 189: 85-90.
  • 20. Verim L, Toptas B, Ozkan NE, Cacina C, Turan S, Korkmaz G, Yaylim I. Possible relation between the NOS3 gene GLU298ASP polymorphism and bladder cancer in Turkey. Asian Pac J Cancer P 2013; 14: 665-668.
  • 21. Wang XL, Sim AS, Wang MX, Murrell GA, Trudinger B, Wang J. Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity. FEBS Lett 2000; 471: 45-50.
  • 22. Akar N, Akar E, CinS Deda G, Avcu F, Yalçin A. Endothelial nitric oxide synthase intron 4, 27 bp repeat polymorphism in Turkish patients with deep vein thrombosis and cerebrovascular accidents. Thromb Res 1999; 94: 63-64.
  • 23. Olcay A, Ekmekci CG, Ozbek U, Sezer M, Barcin C, Arslan E, Boztosun B, Nisanci Y. Negative association of endothelial nitric oxide gene polymorphism with hypertension in Turkish patients: effect of ecNOS polymorphism on left ventricular hypertrophy. Cardiovasc Ultrasound 2006; 4: 33.
  • 24. Sinici I, E. Güven O, Serefoglu E, Hayran M. T-786C Polymorphism in promoter of eNOS gene as genetic risk factor in patients with erectile dysfunction in Turkish population. Urology 2010; 75: 955–960.
  • 25. Tsukada T, Yokoyama K, Arai T, Takemoto F, Hara S, Yamada A, Kawaguchi Y, Hosoya T, Igari J. Evidence of association of the ecNOS gene polymorphism with plasma NO metabolite levels in humans. Biochem Bioph Res Co 1998; 245: 190-193.
  • 26. Song J, Yoon Y, Park KU, Park J, Hong YJ, Hong SH, Kim JQ. Genotype-specific influence on nitric oxide synthase gene expression, protein concentrations, and enzyme activity in cultured human endothelial cells. Clin Chem 2003; 9: 847-852.
APA Polat F, TURAÇLAR N, YILMAZ m, Bingöl G, CİNGİLLİ VURAL H (2016). eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. , 673 - 679.
Chicago Polat Fikriye,TURAÇLAR Nesrin,YILMAZ meral,Bingöl Günsel,CİNGİLLİ VURAL Hasibe eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. (2016): 673 - 679.
MLA Polat Fikriye,TURAÇLAR Nesrin,YILMAZ meral,Bingöl Günsel,CİNGİLLİ VURAL Hasibe eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. , 2016, ss.673 - 679.
AMA Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. . 2016; 673 - 679.
Vancouver Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. . 2016; 673 - 679.
IEEE Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H "eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients." , ss.673 - 679, 2016.
ISNAD Polat, Fikriye vd. "eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients". (2016), 673-679.
APA Polat F, TURAÇLAR N, YILMAZ m, Bingöl G, CİNGİLLİ VURAL H (2016). eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. Turkish Journal of Medical Sciences, 46(3), 673 - 679.
Chicago Polat Fikriye,TURAÇLAR Nesrin,YILMAZ meral,Bingöl Günsel,CİNGİLLİ VURAL Hasibe eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. Turkish Journal of Medical Sciences 46, no.3 (2016): 673 - 679.
MLA Polat Fikriye,TURAÇLAR Nesrin,YILMAZ meral,Bingöl Günsel,CİNGİLLİ VURAL Hasibe eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. Turkish Journal of Medical Sciences, vol.46, no.3, 2016, ss.673 - 679.
AMA Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. Turkish Journal of Medical Sciences. 2016; 46(3): 673 - 679.
Vancouver Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients. Turkish Journal of Medical Sciences. 2016; 46(3): 673 - 679.
IEEE Polat F,TURAÇLAR N,YILMAZ m,Bingöl G,CİNGİLLİ VURAL H "eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients." Turkish Journal of Medical Sciences, 46, ss.673 - 679, 2016.
ISNAD Polat, Fikriye vd. "eNOS gene polymorphisms in paraffin-embedded tissues of prostate cancer patients". Turkish Journal of Medical Sciences 46/3 (2016), 673-679.