EDA GÜNEŞ
(Konya Necmettin Erbakan University)
Ender BÜYÜKGÜZEL
(Bülent Ecevit University)
Yıl: 2017Cilt: 41Sayı: 1ISSN: 1010-6960 / 2536-491XSayfa Aralığı: 3 - 15Türkçe

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Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae)’in farklı gelişme dönemleri üzerine Borik asitin oksidatif etkileri
Tarımsal zararlılar ile mücadelede sentetik organik insektisitler yoğun olarak kullanılmaktadır. Borik asit organik kimyasal insektisitlere göre hedef olmayan organizmalara karşı daha düşük toksisiteye sahip olması nedeniyle önem taşımaktadır. Bu amaçla çalışmamızda meyve sineği Drosophila melanogaster Meigen,1830 (Diptera: Drosophilidae)'in birinci dönem larvaları borik asitin farklı konsantrasyonlarını (10, 100, 200 ve 300 mg/L) içeren yapay besinler ile yetiştirilmiştir. Drosophila melanogaster' in üçüncü dönem larva, pupa, ergin dönemleri ve yumurtalarında oksidatif stresin önemli indikatörleri olan lipid peroksidasyonu ürünü malondialdehid (MDA) ve protein oksidasyon ürünü protein karbonil miktarları (PCO) ile detoksifikasyon enzimi glutatyon-S-transferaz (GST) aktivitesi üzerine etkisi incelenmiştir. Borik asitin denenen konsantrasyonlarını içeren yapay besinler ile yetiştirilen D. melanogaster' in üçüncü dönem larvalarının MDA miktarı önemli derecede artmıştır. Yüksek borik asit ile yetiştirilen erginlerin 10 gün süreyle 300 mg/L borik asit içeren besin ile beslenilmesi sonucunda kontrol besinine göre dişi ve erkek bireylerde MDA ve PCO miktarları önemli derecede artmıştır. Bu dişilerin yumurtalarındaki MDA, PCO miktarları ve GST aktivitesi önemli derecede artarken, PCO miktarındaki artış yaklaşık 31 katı oranında olmuştur. Sonuçlarımız, borik asitin yüksek konsantrasyonlarıyla beslenen D. melanogaster'in tüm gelişme dönemlerindeki oksidatif stres indikatörleri ve detoksifikasyon enzimi üzerine oldukça etkili olduğunu göstermiştir
Fen > Ziraat > Ziraat Mühendisliği
Fen > Temel Bilimler > Entomoloji
Fen > Tıp > Biyokimya ve Moleküler Biyoloji
DergiAraştırma MakalesiErişime Açık
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