Yıl: 2017 Cilt: 42 Sayı: 4 Sayfa Aralığı: 445 - 450 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells

Öz:
Amaç: Doğal bir bileşik olan D-pinitol, çeşitli biyolojik ve famakolojik etkilere sahiptir. Son çalışmalar, başlıca biyolojik aktiviteyi de kapsayan farmakolojik aktivitelerinin belirlenmesi üzerine odaklanmıştır. Bu nedenle, çalışma kapsamında Colutea cilicica Boiss et Bal. bitkisinin yapraklarından D-pinitol'ün izolasyonu, K562 hücre hattına karşı apoptotik ve antiinflamatuvar aktivitesinin belirlenmesi amaçlanmıştır.Gereç ve Yöntemler: D-pinitol'ün izolasyonu kolon kromatografisi ile gerçekleştirildi. Saf bileşiğin kimyasal yapısı 1H NMR, 13C NMR, 2D NMR, HPLC-TOF/MS ve IR gibi spektroskopik yöntemler ile kesinleştirildi. Hücre canlılığı K562 hücrelerinde zamana ve doza bağlı olarak değerlendirildi. D-pinitol, C. cilicica yapraklarından ilk defa izole edildi.Bulgular: D- pinitol (0-80 ?M) ile hücrelerin uyarılması 24, 48 ve 72  saat boyunca gözlendi. D-pinitol'ün, K562  hücrelerinde Cox-2 protein ekspresyonunu inhibe ettiği belirlendi. D-pinitol'ün sitotoksik olmayan konsantrasyonlarında PARP protein ekspresyonu değişmezken, Cox-2 protein ekspresyonunun azaldığı gözlemlendi.Sonuç: D-pinitol'ün K562  hücrelerinde hücre proliferasyonu ve apoptozu etkilemediği ancak belirgin biçimde inflamasyonu azalttığı sonucuna varıldı. Bu sonuçlar bize D- pinitol'ün K562 hücrelerinin tedavisi için anti-inflamatuar bir ajan olabileceğini göstermektedir
Anahtar Kelime:

Konular: Biyoloji Fizik, Uygulamalı Genel ve Dahili Tıp Biyoteknoloji ve Uygulamalı Mikrobiyoloji Genetik ve Kalıtım Biyokimya ve Moleküler Biyoloji Hücre Biyolojisi

Colutea cilicica Boiss et Bal. yapraklarından izole edilen D-pinitol’ün K562 hücreleri üzerindeki antiinflamatuar etkisi

Öz:
Aim: D-pinitol, a natural compound has shown various biological and pharmacological effects. Last studies are focused on the determination of its further pharmacological activities including mainly biological activity. Therefore, isolation of D-pinitol from the leaves of Colutea cilicica Boiss et Bal. and investigation of its apoptotic and anti-inflammatory activity on K562 cell lines were aimed in the concept of the study.Materials and methods: Isolation of D-pinitol was performed by column chromatography. Chemical structure of the compound was confirmed by spectroscopic methods including 1H NMR, 13C NMR, 2D NMR, HPLC-TOF/MS, and IR. Cell viability was evaluated by dose and time dependent in K562 cell lines. D-pinitol was isolated from C. cilicica leaves for the first time
Anahtar Kelime:

Konular: Biyoloji Fizik, Uygulamalı Genel ve Dahili Tıp Biyoteknoloji ve Uygulamalı Mikrobiyoloji Genetik ve Kalıtım Biyokimya ve Moleküler Biyoloji Hücre Biyolojisi
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ESER F, ALTUNDAĞ E, GEDİK G, DEMİRTAŞ İ, onal a, SELVİ B (2017). Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. , 445 - 450.
Chicago ESER FERDA,ALTUNDAĞ Ergül Mutlu,GEDİK GÜLŞAH,DEMİRTAŞ İbrahim,onal adem,SELVİ BEDRETTİN Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. (2017): 445 - 450.
MLA ESER FERDA,ALTUNDAĞ Ergül Mutlu,GEDİK GÜLŞAH,DEMİRTAŞ İbrahim,onal adem,SELVİ BEDRETTİN Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. , 2017, ss.445 - 450.
AMA ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. . 2017; 445 - 450.
Vancouver ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. . 2017; 445 - 450.
IEEE ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B "Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells." , ss.445 - 450, 2017.
ISNAD ESER, FERDA vd. "Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells". (2017), 445-450.
APA ESER F, ALTUNDAĞ E, GEDİK G, DEMİRTAŞ İ, onal a, SELVİ B (2017). Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. Türk Biyokimya Dergisi, 42(4), 445 - 450.
Chicago ESER FERDA,ALTUNDAĞ Ergül Mutlu,GEDİK GÜLŞAH,DEMİRTAŞ İbrahim,onal adem,SELVİ BEDRETTİN Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. Türk Biyokimya Dergisi 42, no.4 (2017): 445 - 450.
MLA ESER FERDA,ALTUNDAĞ Ergül Mutlu,GEDİK GÜLŞAH,DEMİRTAŞ İbrahim,onal adem,SELVİ BEDRETTİN Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. Türk Biyokimya Dergisi, vol.42, no.4, 2017, ss.445 - 450.
AMA ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. Türk Biyokimya Dergisi. 2017; 42(4): 445 - 450.
Vancouver ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells. Türk Biyokimya Dergisi. 2017; 42(4): 445 - 450.
IEEE ESER F,ALTUNDAĞ E,GEDİK G,DEMİRTAŞ İ,onal a,SELVİ B "Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells." Türk Biyokimya Dergisi, 42, ss.445 - 450, 2017.
ISNAD ESER, FERDA vd. "Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells". Türk Biyokimya Dergisi 42/4 (2017), 445-450.