This study was conducted in order to determine the effect of increasing doses of sulfur (S) application on somemacro and micro elements, vitamin C, protein contents and biological properties of canola plant. In the experimentfour doses of (50: mg kg'ı, Sl: 20 mg kg'l, SZ: 40 mg kg'ı, 53: 60 mg kg'ı) were applied as NH4SO4 fertilizer ingreenhouse conditions. The content of Vitamin content of canola plant decreased with increasing dosesapplication of S. Vitamin content of canola plant was obtained for So dose as 28.41 mg 100'1g and decreasedVitamin content of canola plant with increasing application and 22.36 mg 100'1g obtained for 53 dose. Proteincontent of canola plant increased from So dose to SZ dose with application and decreased for 53 dose application.Protein content of canola plant was found 33.71 for 52 dose. On the other hand, Potassium (K), Calcium (Ca),Magnesium (Mg), Iron (Fe) and Copper (Cu) nutrient elements contents of canola plant increased with applicationbut these increases were not found statistically significant. The highest Nitrogen (N) (6.68 %), Phosphorus (P) (0.80%) and Zinc (Zn) (47.4 mg kg'ı) contents were obtained with 52 dose and the highest Mangan (Mn) (70.6 mg kg'ı)content were obtained with 53 dose of canola plant. Some biological properties of canola plant increased withincreasing sulfur application. Plant height was determined as 37.16 cm, plant weight as 101.87g and number ofleaves plant as 8.33 for S3 dose application. Canola plant can be an alternative product rather for vegetables whichhave rapid growth periods such as lettuce, cress, rocket, fresh onion, garlic, and leek from the months of late fall toearly spring even until the beginning of summer.