ntroduction: In order to detect blood stream infections, bloodcultures are essential. There is a proven relation between thenumber of blood cultures and growing rates. Guidelines offer that2 to 4 blood cultures should be obtained over a 24 hours period.The aim of this study was to examine the investigation ofsuitability of number of blood culture bottles.Material and Methods: The study was conducted in InfectiousDiseases and Clinical Microbiology Laboratory of KirikkaleUniversity Faculty of Medicine. The number of blood culturesobtained from each hospitalized and admitted patients to ourlaboratory were evaluated from March 2013 to March 2014. Inaccordance with guidelines, 2 to 4 blood cultures were accepted asappropriate. Blood cultures were divided into two groupsaccording to admission from internal diseases clinics or surgicalclinics. SPSS 15.0 program was used for data analysis and chisquaretest was used to compare the groups. P<0.05 was acceptedas significant.Results: During the study period, 1095 blood cultures from 621patients had been admitted to our laboratory. 192 patients werestaying in surgical clinics and 429 were in internal diseases clinics.435 patients had suitable number (2-4) of blood cultures. While asingle blood culture was obtained from 185 patients, more than 4cultures were obtained from 1patient. Unsuitable number of bloodcultures was 66 in surgical clinics and 120 in internal diseasesclinics (p=0.107).Conclusion: According to our results all hospitals should detecttheir suitability of number of blood cultures and educationprograms should be conducted accordingly.