Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience
Yıl: 2017 Cilt: 47 Sayı: 1 Sayfa Aralığı: 21 - 25 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience
Öz: Amaç: Antibiyotik duyarlılık testleri (ADT) akılcı antibiyotik kullanımı için önemli bir katkı sağlamaktadır. Bu testlerin uygulanmasında, iki standart rehber tüm dünyada yaygın olarak kullanılmaktadır. Ülkemizde son yıllara kadar CLSI Standartları kullanılmakta iken, artık EUCAST standartlarına geçiş süreci başlamıştır. Yeni standartlara geçilmesiyle birlikte, güncel epidemiyolojik verilerin eskileriyle karşılaştırılmasında hangi antibiyotiklere dikkat edilmesi gerektiği ortaya konmalıdır. Bu çalışmanın amacı, CLSI ve EUCAST rehberlerindeki sınır değeri değişikliklerinin ADT raporlarındaki etkisini incelemektir.Gereç ve Yöntem: Bu çalışmada 1 Ocak 2012 ve 31 Aralık 2013 arasında Hacettepe Üniversitesi Tıp Fakültesi Hastanesi Klinik Mikrobiyoloji Laboratuvarı'nda izole edilen ve ADT'leri CLSI ile yorumlanmış olan toplam 1650 izolattaki direnç oranları EUCAST standartlarına göre yeniden değerlendirilmiştir.Bulgular: CLSI ve EUCAST standartları ile alınan sonuçlar karşılaştırıldığında direnç yüzdeleri birbirine yakın bulunmuştur. Bununla birlikte, en büyük fark Pseudomonas aeruginosa`da levofloksasin için %9.7 olarak saptanmıştır. Ayrıca P. aeruginosa için siprofloksasinde %7 fark saptanmıştır. Acinetobacter baumannii izolatlarında amikasinde (%2.8) ve levofloksasinde (%1.7) az oranda fark bulunmuştur. Escherichia coli izolatları için de siprofloksasin (%1.2), amoksisilin/klavulanat (%2.5), sefepim (%1.6) ve seftazidimde (%4.2) az oranda farklılıklar bulunmuştur.Sonuç: Bu çalışmada, ülkemizde EUCAST standartlarına geçiş sürecinde CLSI ile yorumlanmış olan sonuçlar EUCAST rehberlerinde önerilen direnç sınır değerlerine göre yine yorumlanmış ve farklar ortaya konmuştur. EUCAST standartları uygulanırken bu farklılıklar göz önünde bulundurulmalıdır
Anahtar Kelime: Konular:
Pseudomonas aeruginosa, Acinetobacter baumannii ve Escherichia coli İzolatlarının Antibiyotik Duyarlılık Test Sonuçlarının EUCAST ve CLSI’ya Göre Yorumlanması: Hacettepe Deneyimi
Öz: Interpretation of Antibiotic Susceptibility Test Results ofPseudomonas aeruginosa, Acinetobacter baumannii andEscherichia coli Isolates According to EUCAST and CLSI:Hacettepe ExperienceObjective: Antibiotic susceptibility tests (AST) provide an importantcontribution to the rational use of antibiotics. Two standard guidelinesare commonly used for the application of test results worldwide. Inour country CLSI guidelines have been used up to recent years, butcurrently a transition to implementation of EUCAST standards hasbegun. With the adoption of the new standards, the currentepidemiological data should be compared with the previous data so asto to reveal the antibiotics which should be paid attention . The aim ofthis study was to analyse the effects of changes in clinical cut-offvalues in CLSI and EUCAST guidelines on AST reports.Material and Methods: A total of 1650 antimicrobial test resultswhich were reported according to CLSI standards in HacettepeUniversity Clinical Microbiological Laboratory from 1 January2012 to 31 December 2013 were included in the study.Results: Resistance rates were found to be nearly similar accordingto CLSI and EUCAST standards. Highest difference was found inPseudomonas aeruginosa and levofloxacin (9.7%); also therewere differences in ciprofloxacin (7%) for P. aeruginosa. InAcinetobacter baumannii there were minor differences in amikacin(2.8%) and levofloxacin (1.7%). In Escherichia coli, ciprofloxacin(1.2%), amoxicillin-clavulanate (2.5%), cefepime (1.6%) andceftazidime (4.2%) differed slightly in EUCAST.Conclusion: In our country, CLSI guidelines are being replaced byEUCAST guidelines. In this study, antimicrobial susceptibilityresults interpreted by CLSI guidelines were reinterpreted accordingto EUCAST cut-off values and changes in the susceptibilitycategories were assessed. These differences should be taken intoaccount when EUCAST standards are employed.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | MAÇIN S, AKYÖN YILMAZ Y, ÖZDEN Ö, Gur D (2017). Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. , 21 - 25. |
| Chicago | MAÇIN SALIH,AKYÖN YILMAZ YAKUT,ÖZDEN Özben,Gur Deniz Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. (2017): 21 - 25. |
| MLA | MAÇIN SALIH,AKYÖN YILMAZ YAKUT,ÖZDEN Özben,Gur Deniz Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. , 2017, ss.21 - 25. |
| AMA | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. . 2017; 21 - 25. |
| Vancouver | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. . 2017; 21 - 25. |
| IEEE | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D "Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience." , ss.21 - 25, 2017. |
| ISNAD | MAÇIN, SALIH vd. "Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience". (2017), 21-25. |
| APA | MAÇIN S, AKYÖN YILMAZ Y, ÖZDEN Ö, Gur D (2017). Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. Türk Mikrobiyoloji Cemiyeti Dergisi, 47(1), 21 - 25. |
| Chicago | MAÇIN SALIH,AKYÖN YILMAZ YAKUT,ÖZDEN Özben,Gur Deniz Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. Türk Mikrobiyoloji Cemiyeti Dergisi 47, no.1 (2017): 21 - 25. |
| MLA | MAÇIN SALIH,AKYÖN YILMAZ YAKUT,ÖZDEN Özben,Gur Deniz Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. Türk Mikrobiyoloji Cemiyeti Dergisi, vol.47, no.1, 2017, ss.21 - 25. |
| AMA | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. Türk Mikrobiyoloji Cemiyeti Dergisi. 2017; 47(1): 21 - 25. |
| Vancouver | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience. Türk Mikrobiyoloji Cemiyeti Dergisi. 2017; 47(1): 21 - 25. |
| IEEE | MAÇIN S,AKYÖN YILMAZ Y,ÖZDEN Ö,Gur D "Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience." Türk Mikrobiyoloji Cemiyeti Dergisi, 47, ss.21 - 25, 2017. |
| ISNAD | MAÇIN, SALIH vd. "Interpretation of Antibiotic Susceptibility Test Results of Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli Isolates According to EUCAST and CLSI: Hacettepe Experience". Türk Mikrobiyoloji Cemiyeti Dergisi 47/1 (2017), 21-25. |
