Yıl: 2018 Cilt: 8 Sayı: 1 Sayfa Aralığı: 69 - 74 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi

Öz:
Kenodeoksikolik asitin 7b epimeri olan ursodeoksikolik asit (UDKA), kolestatik karaciğer hastalıklarının tedavilerinde artan bir şekilde kullanılmaktadır. Bu çalışmada, Ursodeoksikolik asitin, insan periferal kan lenfositlerindeki in vitro sitotoksik ve genotoksik etkilerinin belirlenmesi hedeflendi. UDKA'nın potansiyel genotoksik ve sitotoksik etkisi, kromozom aberasyon ve mitotik indeks testleri kullanılarak in vitro olarak araştırıldı. İnsan peripheral kan lenfositleri, 24 ve 48 saat süreyle, 10, 50 ve 100 µg/ml UDKA ile muamele edildi. Veriler SPSS istatistik programında, Tek Yönlü Anova (Post Hoc Analiz-LSD Test) testi ile analiz edildi. Elde edilen istatistik sonuçları, kontrolle karşılaştırıldığında, uygulanan UDKA konsantrasyonlarının mitotik indeks değerlerini düşürmediğini ve kromozom anomali frekanslarında da önemli bir artışa neden olmadığını göstermektedir (p>0.05). UDKA'nın insan kromozomlarında anomalileri artırmaması bulgusu, bu maddenin az da olsa insan vücudunda fizyolojik olarak üretilen bir safra asiti olması ile bağlantılı olabilir. UDKA'yı kullanan pek çok hasta olması, bu çalışmadan elde ettiğimiz bulguların önemini artırmaktadır
Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji

In Vitro Genotoxic Effects of Ursodeoxycholic Acid in Human Peripheral Blood Lymphocytes

Öz:
Ursodeoxycholic acid (UDCA), the 7b epimer of chenodeoxycholic acid has been increasingly used for the treatment of cholestatic liver diseases. In this study, it was aimed to determine the potential in vitro genotoxic effects of ursodeoxycholic acid in human peripheral blood lymphocytes. The potential genotoxic and cytotoxic effects of UDCA were investigated in vitro by using chromosome aberration and mitotic index assays. Human peripheral blood lymphocytes were treated with 10, 50 and 100 µg/ml of UDCA for 24 and 48 hours. The data were evaluated using one-way ANOVA (Post Hoc Analysis-LSD Test) test in the SPSS statistics program. Statistical results showed that the applied doses of UDCA did not cause a significant increase in chromosome aberration frequency, and also it did not cause a significant decrease in mitotic index when compared to the control (p>0.05). The finding that UDCA does not cause an increase in chromosomal aberrations may be related to the production of this substance physiologically as bile acid in the human body, even in trace amount. The fact that there are many patients using UDCA increases the importance of findings obtained from this study
Anahtar Kelime:

Konular: Biyoloji Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA BUDAK-DİLER S, Polat F, ÖZER E (2018). Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. , 69 - 74.
Chicago BUDAK-DİLER SONGÜL,Polat Fikriye,ÖZER Eyüp Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. (2018): 69 - 74.
MLA BUDAK-DİLER SONGÜL,Polat Fikriye,ÖZER Eyüp Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. , 2018, ss.69 - 74.
AMA BUDAK-DİLER S,Polat F,ÖZER E Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. . 2018; 69 - 74.
Vancouver BUDAK-DİLER S,Polat F,ÖZER E Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. . 2018; 69 - 74.
IEEE BUDAK-DİLER S,Polat F,ÖZER E "Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi." , ss.69 - 74, 2018.
ISNAD BUDAK-DİLER, SONGÜL vd. "Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi". (2018), 69-74.
APA BUDAK-DİLER S, Polat F, ÖZER E (2018). Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(1), 69 - 74.
Chicago BUDAK-DİLER SONGÜL,Polat Fikriye,ÖZER Eyüp Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8, no.1 (2018): 69 - 74.
MLA BUDAK-DİLER SONGÜL,Polat Fikriye,ÖZER Eyüp Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.8, no.1, 2018, ss.69 - 74.
AMA BUDAK-DİLER S,Polat F,ÖZER E Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 8(1): 69 - 74.
Vancouver BUDAK-DİLER S,Polat F,ÖZER E Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 8(1): 69 - 74.
IEEE BUDAK-DİLER S,Polat F,ÖZER E "Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi." Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8, ss.69 - 74, 2018.
ISNAD BUDAK-DİLER, SONGÜL vd. "Ursodeoksikolik Asit’in İnsan Periferal Kan Lenfositlerindeki in Vitro Genotoksik Etkisi". Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8/1 (2018), 69-74.