A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats

Keser, Selçuk; SONGÜR, Murat; DORAL, Mahmut Nedim; TURHAN, Egemen; BAYAR, Ahmet; AKÇA, Mustafa Kemal

A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats

Egemen TURHAN, (Department of Orthopedics and Traumatology, Hacettepe University Faculty of Medicine, Ankara-Turkey)

Mustafa Kemal AKÇA, (Department of Orthopedics and Traumatology, Bandırma Government Hospital, Balıkesir-Turkey)

Ahmet BAYAR, (Department of Orthopedics and Traumatology, Bülent Ecevit Faculty of Medicine, Zonguldak-Turkey)

Murat SONGÜR, (Department of Orthopedics and Traumatology, Bülent Ecevit Faculty of Medicine, Zonguldak-Turkey)

Selçuk KESER, (Department of Orthopedics and Traumatology, Bülent Ecevit Faculty of Medicine, Zonguldak-Turkey)

Mahmut Nedim DORAL (Department of Orthopedics and Traumatology, Hacettepe University Faculty of Medicine, Ankara-Turkey)

Ulusal Travma ve Acil Cerrahi Dergisi

6 0

Yıl: 2017 Cilt: 23 Sayı: 2 Sayfa Aralığı: 91 - 99 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats

Öz:

BACKGROUND: Delayed union of fractured bone is one of the main problems of orthopedics and traumatology practice. It was hypothesized that the beneficial effects of allogeneic platelet-rich plasma (PRP) would be valuable in the treatment of segmental bone defects. This study is a comparison of the effects of demineralized bone matrix (DBM) and PRP in a segmental bone defect model.METHODS: Total of 48 Wistar albino rats were separated into 4 groups. Segmental bone defect was created at right radius diaphysis in all specimens using dorsal approach. Four additional rats were used as PRP source. Intracardiac blood was withdrawn before the operation for preparation of allogeneic PRP. Group 1 (n=12) served as control group and defects were left untreated. Group 2 (n=12), was PRP group, and received grafting with PRP. Group 3 (n=12) was PRP+DBM combination group, and was treated with grafting and mixture of DBM and PRP. In Group 4 (n=12), defect area was grafted with DBM only. At the end of 10th week, rats were sacrificed, forearms were dissected, and defect areas were examined with radiological and histopathological parameters.RESULTS: Radiological evaluation revealed that ossification was best in PRP group, followed by DBM group. According to results of histopathological studies, union quality was better than control group in all treatment groups (Groups 2, 3, and 4), and was best in PRP group (p<0.05). Results were also better in PRP group when examined in terms of cortex development and remodeling (p<0.05). When examined in terms of new osteogenesis, results were comparable in Groups 2, 3, and 4, but all were better than control group.CONCLUSION: It was concluded that PRP and DBM have comparable effect on recovery of defective bones, but there is no synergistic effect when used together. We believe that PRP can be a cost-effective, readily available alternative to DBM with minimal morbidity

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Konular: Cerrahi

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APA	TURHAN E, AKÇA M, BAYAR A, SONGÜR M, Keser S, DORAL M (2017). A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. , 91 - 99.
Chicago	TURHAN Egemen,AKÇA Mustafa Kemal,BAYAR Ahmet,SONGÜR Murat,Keser Selçuk,DORAL Mahmut Nedim A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. (2017): 91 - 99.
MLA	TURHAN Egemen,AKÇA Mustafa Kemal,BAYAR Ahmet,SONGÜR Murat,Keser Selçuk,DORAL Mahmut Nedim A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. , 2017, ss.91 - 99.
AMA	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. . 2017; 91 - 99.
Vancouver	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. . 2017; 91 - 99.
IEEE	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M "A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats." , ss.91 - 99, 2017.
ISNAD	TURHAN, Egemen vd. "A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats". (2017), 91-99.

APA	TURHAN E, AKÇA M, BAYAR A, SONGÜR M, Keser S, DORAL M (2017). A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. Ulusal Travma ve Acil Cerrahi Dergisi, 23(2), 91 - 99.
Chicago	TURHAN Egemen,AKÇA Mustafa Kemal,BAYAR Ahmet,SONGÜR Murat,Keser Selçuk,DORAL Mahmut Nedim A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. Ulusal Travma ve Acil Cerrahi Dergisi 23, no.2 (2017): 91 - 99.
MLA	TURHAN Egemen,AKÇA Mustafa Kemal,BAYAR Ahmet,SONGÜR Murat,Keser Selçuk,DORAL Mahmut Nedim A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. Ulusal Travma ve Acil Cerrahi Dergisi, vol.23, no.2, 2017, ss.91 - 99.
AMA	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2017; 23(2): 91 - 99.
Vancouver	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2017; 23(2): 91 - 99.
IEEE	TURHAN E,AKÇA M,BAYAR A,SONGÜR M,Keser S,DORAL M "A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats." Ulusal Travma ve Acil Cerrahi Dergisi, 23, ss.91 - 99, 2017.
ISNAD	TURHAN, Egemen vd. "A comparison of the effects of platelet-rich plasma and demineralized bone matrix on critical bone defects: An experimental study on rats". Ulusal Travma ve Acil Cerrahi Dergisi 23/2 (2017), 91-99.