Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats

Sahin, Nurhan; ÖZEROL, Elif; Bilgiç, Sedat; Tanbek, Kevser; IRAZ, Mustafa; ÇOLAK, Cemil

Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats

Kevser TANBEK, (Department of Biochemistry, Inonu University School of Medicine, Malatya, Turkey)

Elif ÖZEROL, (Department of Biochemistry, Inonu University School of Medicine, Malatya, Turkey)

SEDAT BİLGİÇ, (Department of Biochemistry, Adıyaman University Vocational School of Health Services, Adıyaman, Turkey)

Mustafa IRAZ, (Department of Pharmacology, Istanbul Medeniyet University, Medical Faculty, Istanbul, Turkey)

Nurhan ŞAHİN, (Department of Pathology, Inonu University School of Medicine, Malatya, Turkey)

CEMİL ÇOLAK (Department of Bioistatistics, Inonu University School of Medicine, Malatya, Turkey)

Medicine Science

9 1

Yıl: 2017 Cilt: 6 Sayı: 1 Sayfa Aralığı: 96 - 103 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats

Öz:

The aim of this study was to investigate the possible protective effect of Nigella sativa oil (NSO) and compared with the N-acetylcysteine (NAC), an antioxidant which is known to have liver protective effects, against thioacetamide (TAA)-induced liver injury. Accordingly, we aimed to investigate the toxicity of a hepatotoxic agent, thioacetamide, compared the NSO and NAC liver preventive effects in experimental animal model. Wistar-albino rats were randomly allocated to five groups, each consisting of eight rats, and were subjected to different treatment regimens for 6 days. Alanine aminotransferase (ALT), malondialdehyde (MDA), and protein carbonyl (PC) levels decreased significantly by NSO treatment (P<0.05). Besides total bilirubin (T-Bil), cholesterol, superoxide dismutase (SOD), and catalase (CAT) activities increased significantly by NSO treatment (P<0.05). NSO treatment did not produce a significant effect on myeloperoxidase (MPO), hydroxyproline (OH-P), and glutathione peroxidase (GSH-Px) activities. These results indicated that NSO improved the liver cell damage caused by TAA, protected against oxidative tissue-damage, contributed to the oxygen radical scavenging activity, increased antioxidant activity and decreased lipid peroxidation. Thus, these results indicate that NSO has protective and antioxidant effects as in the treatment of NAC on the liver injury in rats

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Khan MA. Chemical composition and medicinal properties of Nigella sativa Linn. Inflammopharmacology. 1999;7(1):15-35.
2. Salem ML. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology. 2005;5(13-14):1749-70.
3. Mohamadin AM, Sheikh B, Abd El-Aal AA, Elberry AA, Al Abbasi FA. Protective effects of Nigella sativa oil on propoxur induced toxicity. Pest Biochem Physiol. 2010;98:128-34.
4. Cheikh-Rouhou S, Besbes S, Blecker C, Deroanne C, Attia H. Nigella sativa L. Chemical composition and physicochemical characteristics of lipid fraction. Food Chem. 2007;101:673-81.
5. Bourgou S, Ksouri R, Bellila A, Skandran I, Falleh H, Marzouk B. Phenolic composition and biological activities of Tunisian Nigella sativa L shots and roots. C Biol. 2008;331(1):48–55.
6. Ashraf SS, Rao MV, Kaneez FS. Nigella sativa extract as a potent antioxidant for petrochemical-induced oxidative stress. J Chromatogr Sci. 2011;49(4):321-6.
7. Kanter M. Nigella sativa and derived thymoquinone prevents hippocampal neurodegeneration after chronic toluene exposure in rats. Neurochem Res. 2008;33(3):579-88.
8. Al-Ghamdi MS. Protective effect of Nigella sativa seeds against carbontetrachloride-induced liver damage. Am J Chin Med. 2003;31(5): 721-8.
9. Mahmoud MR, El-Abhar HS, Saleh S. The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J Ethnopharmacol. 2002;79(1):1-11.
10. Gali-Muhtasıb H, El-Najjar N, Schneider-Stock R. The medicinalpotential of black seed (Nigella sativa) and its components. In: Khan MTH, Ather A, eds, Lead Molecules from Natural Products: Discovery and NewTrends. Elsevier BV, 2006;133-53.
11. Salem ML. Immunomodulatory and therapeutic properties of the Nigellasativa L. seed. Internet Immunopharmacol. 2005;5(13- 14):1749-70.
12. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigellasativa. Phytother Res. 2003;17(4):299-305.
13. ML Salem. Immunomodulatory and therapeutic properties of the Nigellasativa L. seed. Int. Immunopharmacol. 2005;5:1749-70.
14. MA Khan. Chemical composition and medicinal properties of Nigella sativa Linn. Inflammopharmacology. 1999;7(1):15-35.
15. Doğru-Abbasoğlu S, Kanbağlı Ö, Balkan J, Çevikbaş U, Aykaç- Toker G, Uysal M. The protective effect of taurine against thioacetamide hepatotoxicity of rats. Human Exp Toxicol. 2001;20(1):23-7.
16. Balkan J, Doğru-Abbasoğlu S, Kanbağlı Ö, Çevikbaş U, Aykaç- Toker G, Uysal M. Taurine has protective effect against thioacetamide-induced liver cirrhosis by decreasing oxidative stress. Human Exp Toxicol. 2001;20(5):251-4.
17. H Dashti, B Jeppsson, I Hagerstrand. Thioacetamide- and carbontetrachloride-induced liver cirrhosis. Eur Surg Res. 1989;21(2):83-91.
18. Muller A, Machnik F, Zimmermann T, Schubert H. Thioacetamideinduced cirrhosis-like liver lesions in rats-usefulness and reliability of this animal model. Exp Pathol. 1988;34(4):229-36.
19. Aruoma OI, Halliwell B, Hoey BM, Butler J. The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide and hypochlorous acid. Free Radic Biol Med. 1989;6(6):593-7.
20. Shapiro H, Ashkenazi M, Weizman N, Shahmurov M, Aeed H, Bruck R. Curcumin ameliorates acute thioacetamide induced hepatotoxicity. J Gastroenterol Hepatol. 2006;21(2):358-66.
21. Bruck R, Aeed H, Avni Y, Shirin H, Matas Z, Shahmurov M, Avinoach I, Zozulya G, Weizman N, Hochman A. Melatonin inhibits nuclear factor kappa B activation and oxidative stress and protects against thioacetamide induced liver damage in rats. J Hepatol. 2004;40(1):86-93.
22. Kokdil G, Tamer L, Ercan B, Ilcim A, Aras N, Atik U. Effects of Nigella unguicularis fixed oil on blood biochemistry and oxidant/antioxidant balance in rats. J Ethnopharmacol. 2005;99(1):131-5.
23. Aebi H. Catalase. In: Bergmeyer HU ed. Methods of Enzymatic Analysis. New York: Academic Press. 1974;673-7.
24. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
25. Durak I, Yurtaslanı Z, Canbolat O, Akyol O. A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta. 1993;214(1):103-4.
26. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of eryhtrocyte glutatione peroxidase. J Lab Clin Med. 1967;70(1):158-69.
27. Draper HH, Hadley M: Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990;186:421-31.
28. Levine RL, Garland D, Oliver CN. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 1990;186:464- 78.
29. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
30. Renlund DG, Macfarlane JL, Christensen RD, Lynch RE, et al. Quantitative and sensitive method for measurement of myeloperoxidase. Clin Res. 1980;28:75A.
31. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. 1976;72:248-54.
32. Woessner JB. The determination of hydroxyproline in tissue and protein samples containing small proportions of this amino acid. Arch Biochem Biophys. 1961;93:440-7.
33. Domenicali M, Caraceni P, Giannone F, Baldassarre M, Lucchetti G, Quarta C, Parti C, Catani L, Nanni C, Lemoli RM, Bernardi M. A novel model of CCl4-induced cirrhosis with ascites in the mouse. Journal of Hepatology. 2009;51(6):991-9.
34. Pallottini V, Martini C, Bassi AM, Romano P, Nanni G, Trentalance A. Rat HMGCoA reductase activation in thioacetamide-induced liver injury is related to an increased reactive oxygen species content. J Hepatol. 2006;44(2):368-74.
35. Zaoui A, Cherrah Y, Mahassine N, Alaoui K, Amarouch H, Hassar M. Acute and chronic toxicity of Nigella sativa fixed oil. Phytomedicine. 2002;9(1):69-74.
36. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14(5):323-8.
37. Brown KE, Brunt EM and Heinecke JW. Immunohistochemical detection of myeloperoxidase and its oxidation products in Kupffer cells of human liver. Am Journal Pathol. 2001;159(6):2081-8.
38. Uz E, Uz B, Selcoki Y, Bayrak R, Kaya A, Turgut H.F, Mete E, Karanfil A, Ilhan A, Sahin S, Erdemli K. Cardioprotective Effects of Nigella sativa Oil on Cyclosporine A-Induced Cardiotoxicity in Rats. Basic Clin Pharmacol Toxicol. 2008;103(6):574–80.
39. Tayman, C. Cekmez, F. Kafa, I.M. Canpolat, F.E. Cetinkaya, M. Tonbul, A. Uysal, S. Tunc, T. Sarici, S.U. Arch Bronconeumol. 2013;49(1):15-21.
40. Anbarasu C, Rajkapoor B, Bhat KS, John Giridharan, A Arul Amuthan, Satish K. Protective effect of Pisonia aculeata on thioacetamide induced hepatotoxicity in rats. Asian Pac J Trop Biomed. 2012;2(7):511-5.
41. Nissar AU, Farrukh MR, Kaiser PJ, Rafiq RA, Afnan Q, Bhushan S, Adil HS, Subhash BC, Tasduq SA. Effect of N-acetyl cysteine (NAC), an organosulfur compound from Allium plants, on experimentally induced hepatic prefibrogenic events in Wistar rat. Phytomedicine. 2013;20(10):828-33.

APA	Tanbek K, ÖZEROL E, Bilgiç S, IRAZ M, Sahin N, ÇOLAK C (2017). Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. , 96 - 103.
Chicago	Tanbek Kevser,ÖZEROL Elif,Bilgiç Sedat,IRAZ Mustafa,Sahin Nurhan,ÇOLAK Cemil Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. (2017): 96 - 103.
MLA	Tanbek Kevser,ÖZEROL Elif,Bilgiç Sedat,IRAZ Mustafa,Sahin Nurhan,ÇOLAK Cemil Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. , 2017, ss.96 - 103.
AMA	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. . 2017; 96 - 103.
Vancouver	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. . 2017; 96 - 103.
IEEE	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C "Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats." , ss.96 - 103, 2017.
ISNAD	Tanbek, Kevser vd. "Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats". (2017), 96-103.

APA	Tanbek K, ÖZEROL E, Bilgiç S, IRAZ M, Sahin N, ÇOLAK C (2017). Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science, 6(1), 96 - 103.
Chicago	Tanbek Kevser,ÖZEROL Elif,Bilgiç Sedat,IRAZ Mustafa,Sahin Nurhan,ÇOLAK Cemil Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science 6, no.1 (2017): 96 - 103.
MLA	Tanbek Kevser,ÖZEROL Elif,Bilgiç Sedat,IRAZ Mustafa,Sahin Nurhan,ÇOLAK Cemil Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science, vol.6, no.1, 2017, ss.96 - 103.
AMA	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science. 2017; 6(1): 96 - 103.
Vancouver	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats. Medicine Science. 2017; 6(1): 96 - 103.
IEEE	Tanbek K,ÖZEROL E,Bilgiç S,IRAZ M,Sahin N,ÇOLAK C "Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats." Medicine Science, 6, ss.96 - 103, 2017.
ISNAD	Tanbek, Kevser vd. "Protective effect of Nigella sativa oil against thioacetamide-induced liver injury in rats". Medicine Science 6/1 (2017), 96-103.