Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats

MARDİN, Barış; Küçük, Ayşegül; Özer, Abdullah; UNAL, YUSUF; Kılıç, Yiğit; OKTAR, Gürsel Levent; ÇOMU, Faruk Metin; Arslan, Mustafa; alkan, metin

Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats

Abdullah ÖZER, (Department of Cardiovascular Surgery, Gazi University Faculty of Medicine, Ankara, Turkey)

Faruk Metin ÇOMU, (Department of Physiology, Kirikkale University Faculty of Medicine, Kirikkale, Turkey)

Ayşegül KÜÇÜK, (Department of Physiology, Dumlupinar University Faculty of Medicine, Kutahya, Turkey)

Yiğit KILIÇ, (Dr. Siyami Ersek Cardiovascular and Thoracic Surgery Training and Research Hospital, Pediatric Cardiovascular Surgery Clinic, Haydarpasa, Istanbul, Turkey)

Barış MARDİN, (Department of Cardiovascular Surgery, Gazi University Faculty of Medicine, Ankara, Turkey)

Metin ALKAN, (Department of Anaesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara, Turkey)

Gürsel Levent OKTAR, (Department of Cardiovascular Surgery, Gazi University Faculty of Medicine, Ankara, Turkey)

Mustafa ARSLAN, (Department of Anaesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara, Turkey)

Yusuf ÜNAL (Department of Anaesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara, Turkey)

Gazi Medical Journal

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Yıl: 2018 Cilt: 29 Sayı: 2 Sayfa Aralığı: 113 - 115 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats

Öz:

Amaç: İskemi-reperfüzyon (İR) hasarı, doku iskemisi sonucu gerçekleşen bir olaylar zinciridir. Hücresel hasarı takiben reperfüzyon, aktif bir inflamatuar yanıta neden olur. Eritrosit deformabilitesi ve plazma viskozitesi doku ve organ perfüzyonunda kritik bir rol oynar. Bu çalışmada, ratlarda miyokardiyal İR hasarı sırasında levosimendan ve nigella sativa'nın eritrosit deformabilitesi üzerine etkisini araştırmayı amaçladık. Yöntem: Yirmi dört adet Wistar albino rat çalışmaya dahil edildi. Hayvanlar rastgele dört deney grubuna ayrıldı. Grup K'daki (kontrol grubu) ratların koroner arterlerine oklüzyon veya reperfüzyon yapılmadı. Miyokardiyal İR, sol ön inen koroner arterin 30 dakika süreyle bağlanması, ardından İR (İR), İR-levosimendan (24 µg/kg) (İRL) ve İR-nigella sativa (0.2 mL/kg) (İRNS) gruplarında 2 saat reperfüzyon ile gerçekleştirildi. Deformabiliteyi ölçmek için% 5 htc içeren bir PBS tampon çözeltisi ile oluşturulan eritrosit süspansiyonları kullanıldı. Bulgular: Sonuç olarak, kontrol grubuna kıyasla İR, eritrosit deformabilitesinin bir belirteci olan nispi direnci artırdı (p<0.05). Gruplar arasında ANOVA testi ile yapılan karşılaştırmalara göre anlamlı fark vardı (p<0.0001). Bonferroni testi ile yapılan düzeltmelerden sonra elde edilen sonuçlar şu şekildedir: İRL ve İRNS gruplarının karşılaştırmaları benzer sonuçlar vermiştir (p=0.764). İR grubunun değerleri kontrol, İRNS ve İRL gruplarından anlamlı derecede yüksekti (sırasıyla p<0.0001, p=0.001, p=0.003). Sonuç: İskemi reperfüzyon hasarı olan ratlarda eritrosit deformabilitesi azaldı. Bu yaralanma mikrodolaşımda daha fazla sorun yaratabilir. Levosimendan ve nigella sativa, bu tip yaralanmaların olumsuz etkilerini azaltmada yararlı olabilir

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Ratlarda Miyokardiyal İskemi-Reperfüzyon Hasarı Sırasında Levosimendan ve Nigella Sativa'nın Eritrosit Deformabilitesine Etkisi

Öz:

Objective: Ischaemia-reperfusion (IR) injury is a chain of events put in place by tissue ischaemia. Reperfusion following the cellular damage causes an active inﬂammatory response. Erythrocyte deformability and plasma viscosity play a critical role in tissue and organ perfusion. In this study we aimed to evaluate the effect of levosimendan and nigella sativa on erythrocyte deformability during myocardial IR injury in rats. Methods: Twenty-four Wistar albino rats were included in the study. The animals were randomly divided into four experimental groups. The coronary arteries of rats in Group C (control group) were not occluded or reperfused. Myocardial IR was performed by ligating the left anterior descending coronary artery for 30 min, followed by 2 h of reperfusion in the IR (IR), IR-levosimendan (24µg/kg) (IRL) group and IR-nigella sativa (0.2 mL/kg) (IRNS) group. Erythrocyte suspensions formed with a PBS buffer solution containing 5% htc were used to measure the deformability. Results: As a result compared to control group, IR increased the relative resistance, a marker of erythrocyte deformability, significantly (p<0.05). There were significant differences between the groups according to the comparisons with ANOVA test (p<0.0001). The results obtained after corrections with Bonferroni test were as follows: Comparisons of the IRL and IRNS groups revealed similar results (p=0.764). The values of the IR group were significantly higher than those of the control, IRNS and IRL groups (p<0.0001, p=0.001, p=0.003, respectively). Conclusion: Rats with IR injury had decreased erythrocyte deformability. This injury might cause more problems in microcirculation. Levosimendan and nigella sativa may be useful in reducing the adverse effects of this type of injury

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Özer A, ÇOMU F, Küçük A, Kılıç Y, MARDİN B, alkan m, OKTAR G, Arslan M, UNAL Y (2018). Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. , 113 - 115.
Chicago	Özer Abdullah,ÇOMU Faruk Metin,Küçük Ayşegül,Kılıç Yiğit,MARDİN Barış,alkan metin,OKTAR Gürsel Levent,Arslan Mustafa,UNAL YUSUF Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. (2018): 113 - 115.
MLA	Özer Abdullah,ÇOMU Faruk Metin,Küçük Ayşegül,Kılıç Yiğit,MARDİN Barış,alkan metin,OKTAR Gürsel Levent,Arslan Mustafa,UNAL YUSUF Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. , 2018, ss.113 - 115.
AMA	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. . 2018; 113 - 115.
Vancouver	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. . 2018; 113 - 115.
IEEE	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y "Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats." , ss.113 - 115, 2018.
ISNAD	Özer, Abdullah vd. "Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats". (2018), 113-115.

APA	Özer A, ÇOMU F, Küçük A, Kılıç Y, MARDİN B, alkan m, OKTAR G, Arslan M, UNAL Y (2018). Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. Gazi Medical Journal, 29(2), 113 - 115.
Chicago	Özer Abdullah,ÇOMU Faruk Metin,Küçük Ayşegül,Kılıç Yiğit,MARDİN Barış,alkan metin,OKTAR Gürsel Levent,Arslan Mustafa,UNAL YUSUF Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. Gazi Medical Journal 29, no.2 (2018): 113 - 115.
MLA	Özer Abdullah,ÇOMU Faruk Metin,Küçük Ayşegül,Kılıç Yiğit,MARDİN Barış,alkan metin,OKTAR Gürsel Levent,Arslan Mustafa,UNAL YUSUF Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. Gazi Medical Journal, vol.29, no.2, 2018, ss.113 - 115.
AMA	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. Gazi Medical Journal. 2018; 29(2): 113 - 115.
Vancouver	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats. Gazi Medical Journal. 2018; 29(2): 113 - 115.
IEEE	Özer A,ÇOMU F,Küçük A,Kılıç Y,MARDİN B,alkan m,OKTAR G,Arslan M,UNAL Y "Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats." Gazi Medical Journal, 29, ss.113 - 115, 2018.
ISNAD	Özer, Abdullah vd. "Effect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats". Gazi Medical Journal 29/2 (2018), 113-115.