Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit

İŞERİ, LATİFE; ünaldı, özlem; YÜRÜKEN, Zehra; DURMAZ, RIZA

Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit

zehra YÜRÜKEN, (National Public Health Agency, Bursa)

Latife İŞERİ, (Department of Clinical Microbiology, Kırıkkale University Faculty of Medicine, Kırıkkale)

özlem ÜNALDI, (Molecular Microbiology Research and Application Laboratory, National Public Health Agency)

rıza DURMAZ (cMolecular Microbiology Research and Application Laboratory, National Public Health Agency, Molecular Microbiology Research and Application Laboratory, National Public Health Agency, Department of Clinical Microbiology, Yıldırım Beyazit University)

Türkiye Klinikleri Tıp Bilimleri Dergisi

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Yıl: 2016 Cilt: 36 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit

Öz:

Pseudomonas aeruginosa and Acinetobacter baumannii ciddi hastane enfeksiyonlarına neden olur. Bu çalışmada, Üçüncü basamak bir hastanenin, yoğun bakım ünitesinde bu bakterilerin hastalar arasında çapraz geçişini ve antibiyotik duyarlılığını araştırdık. GGeerreeçç vvee YYöönntteemmlleerr:: Ekim 2009 ve Haziran 2010 arasında, hastane enfeksiyonundan sorumlu 33 P. aeruginosa (26 hastadan) ve 48 A. baumannii (41 hastadan) izolatı, hastalardan izole edildi. İzolatlar arası klonal bağlantıları araştır- mak için Pulsed field gel electrophoresis kullanıldı. Amikasin, seftazidim, gentamisin, imipenem, se- fepim, piperasillin/tazobaktam, aztreonam ve meropenem duyarlılıkları disk diffüzyon yöntemi kullanılarak incelendi. BBuullgguullaarr::P. aeruginosa izolatları onsekiz pulsotip, iki veya daha fazla suş içeren beş küme oluşturdu. On üç izolat herhangi bir kümeye dahil değildi. Aynı hastaların tekrarlayan örnek- leri çıkarıldıktan sonra kümeleşme oranı %38,5 olarak hesaplandı. Kırk sekiz A. baumannii izolatı 13 pulsotip oluşturdu. Sekiz pulsotip, beş hastanın tekrarlayan beş örneğini de kapsayan 41 suşu içeren kümeler oluşturdu. Kırk bir farklı hastadan elde edilen izolatlar için kümeleşme oranı %87,8 bulundu. P. aeruginosa suşlarının antibiyotiklere direnç oranları %27-39 aralığındayken A. baumannii suşlarının %45,5-91 aralığındaydı. SSoonnuuçç:: Hastanede uygulanan bir enfeksiyon kontrol programına rağmen, yoğun bakım hastalarında, P. aeruginosa, A. baumannii izolatları çapraz geçişler gösterdi. A. bauman- nii suşlarının antimikrobiyal dirençleri çok yüksekti. Bu bulgular göstermiştir ki; mevcut kontrol pro- gramları yeniden değerlendirilmeli, hastane ve personel durumuna özel değişiklikler yapılmalıdır

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Bir Yoğun Bakım Ünitesinde Hastane Enfeksiyonuna Neden Olan Pseudomonas aeruginosa ve Acinetobacter baumannii İzolatlarının Çapraz Taşınımı ve Antimikrobiyal Direncinin Analizi

Öz:

Pseudomonas aeruginosa and Acinetobacter baumannii cause nosocomial in—fections in intensive care units. We investigated the antimicrobial susceptibility and cross—transmis—sion of these bacteria amongst patients in an intensive care unit. Material and Methods: Thirty—threeP. aeruginosa (from 26 patients) and 48 A. baumannii isolates (from 41 patients) responsible for noso—comial infections were isolated from patients between October 2009 and June 2010. Pulsed field gelelectrophoresis was used to investigate clonal relationship among isolates. Susceptibility to amikacin,ceftazidime, gentamycin, imipenem, cefepime, piperacillin/tazobactam, aztreonam, and meropenemwas examined using the disk diffusion method. Results: P. aeruginosa isolates formed 18 pulsotypes; fiveof these were clusters including or more strains having indistinguishable PFGE patterns and the re—maining 13 were unique. After excluding the repeated samples of the same patients, the clustering ratewas estimated as 38.5%. The 48 A. baumannii isolates formed 13 pulsotypes; eight pulsotypes wereclusters including totally 41 strains of which five were from repeated samples of five patients. Theclustering rate was 87.8% for the isolates obtained from 41 different patients. The antimicrobial resis—tance rates of P. aeruginosa ranged from 27—39%, but were 45.5—91% for A. baumannii isolates.Conclusion: Despite an implemented infection control program, P. aeruginosa and A. baumanniiisolates showed cross—transmission among patients, and the antimicrobial resistance rate of A. bau—mannii isolates was very high. These findings indicate that the current infection control programsshould be reassessed and modifications should be made according to the specific hospital and staffingconditions.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YÜRÜKEN Z, İŞERİ L, ünaldı ö, DURMAZ R (2016). Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. , 1 - 6.
Chicago	YÜRÜKEN Zehra,İŞERİ LATİFE,ünaldı özlem,DURMAZ RIZA Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. (2016): 1 - 6.
MLA	YÜRÜKEN Zehra,İŞERİ LATİFE,ünaldı özlem,DURMAZ RIZA Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. , 2016, ss.1 - 6.
AMA	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. . 2016; 1 - 6.
Vancouver	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. . 2016; 1 - 6.
IEEE	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R "Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit." , ss.1 - 6, 2016.
ISNAD	YÜRÜKEN, Zehra vd. "Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit". (2016), 1-6.

APA	YÜRÜKEN Z, İŞERİ L, ünaldı ö, DURMAZ R (2016). Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. Türkiye Klinikleri Tıp Bilimleri Dergisi, 36(1), 1 - 6.
Chicago	YÜRÜKEN Zehra,İŞERİ LATİFE,ünaldı özlem,DURMAZ RIZA Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. Türkiye Klinikleri Tıp Bilimleri Dergisi 36, no.1 (2016): 1 - 6.
MLA	YÜRÜKEN Zehra,İŞERİ LATİFE,ünaldı özlem,DURMAZ RIZA Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. Türkiye Klinikleri Tıp Bilimleri Dergisi, vol.36, no.1, 2016, ss.1 - 6.
AMA	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. Türkiye Klinikleri Tıp Bilimleri Dergisi. 2016; 36(1): 1 - 6.
Vancouver	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit. Türkiye Klinikleri Tıp Bilimleri Dergisi. 2016; 36(1): 1 - 6.
IEEE	YÜRÜKEN Z,İŞERİ L,ünaldı ö,DURMAZ R "Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit." Türkiye Klinikleri Tıp Bilimleri Dergisi, 36, ss.1 - 6, 2016.
ISNAD	YÜRÜKEN, Zehra vd. "Analysis of Cross-Transmission and Antimicrobial Resistance of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates Causing Nosocomial Infection in an Intensive Care Unit". Türkiye Klinikleri Tıp Bilimleri Dergisi 36/1 (2016), 1-6.