The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR
Yıl: 2018 Cilt: 65 Sayı: 3 Sayfa Aralığı: 305 - 311 Metin Dili: İngilizce DOI: 10.1501/Vetfak_0000002861 İndeks Tarihi: 20-10-2018
The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR
Öz: This study was carried out to investigate the presence of Listeria monocytogenes in samples obtained from a slaughterhouse in Samsun, Turkey. A total of 300 samples (25 samples per month) analyzed by Immunomagnetic Separation (IMS) based on conventional culture method. Then isolates were verified and serotyped by PCR using specific primers. According to the analysis, a total of 18 isolates were identified as L. monocytogenes. Distribution of these isolates were as; 5 isolates (serotype 1/2b or 3b) from 1 cattle carcass, 5 isolates (serotype 1/2b or 3b) from 1 knife, 2 isolates (serotype 4b or 4d, 4e) from 1 knife sharpener, 5 isolates (serotype 1/2a or 3a) from 1 floor sample, 1 isolate (serotype 1/2a or 3a) from 1 cowhide, respectively. As a result, carcasses are considered to pose a hazard to public health due to L. monocytogenes contamination. The development of slaughterhouse conditions and production technology, improving the education level of employees in food safety issues, prevention of contamination of foodborne pathogens to carcasses in slaughterhouses and implementation of effective sanitation methods should be provided.
Anahtar Kelime: Konular:
Sığır kesimhanelerinde Listeria monocytogenes varlığı ve serotiplerinin mPCR tekniğiyle belirlenmesi
Öz: Samsun ilindeki mezbahada Listeria monocytogenes varlığının araştırılması amacıyla yapılan bu çalışma 12 aylık bir dönemi kapsamaktadır. Ayda bir kez olmak üzere her ay 25 örnek alınarak toplam 300 örnek L. monocytogenes varlığı bakımından analiz edildi. Örneklerden L. monocytogenes varlığının araştırılmasında Immunomagnetic Separation (IMS) bazlı klasik kültür tekniği, identifikasyonda biyokimyasal testler kullanıldı. İdentifiye edilen izolatlar PCR ile doğrulandı ve spesifik primerler ile serotiplendirildi. Yapılan biyokimyasal doğrulama testleri ve PCR analizleri sonucunda; 1 karkas örneğinden 5 izolat, 1 bıçak örneğinden 5 izolat; 1 bıçak bileyicisi örneğinden 2 izolat; 1 zemin örneğinden 5 izolat; 1 deri örneğinden 1 izolat olmak üzere toplam 18 izolat L. monocytogenes olarak belirlendi. Yapılan serotiplendirme sonucunda 1 karkas örneğinden elde edilen 5 izolat, 1 bıçak örneğinden elde edilen 5 izolat L. monocytogenes 1/2b (3b); 1 bıçak bileyicisi örneğinden elde edilen 2 izolat L. monocytogenes 4b (4d, 4e); 1 zemin örneğinden elde edilen 5 izolat ve 1 deri örneğinden elde edilen 1 izolat L. monocytogenes 1/2a (3a) olarak tespit edildi. Sonuç olarak karkaslardaki L. monocytogenes kontaminasyonunun halk sağlığı açısından tehlike oluşturabileceği değerlendirilmiştir. Mezbaha şartlarının ve üretim teknolojisinin geliştirilmesi, çalışan personelin gıda güvenliği hususunda eğitim düzeyinin arttırılması ve mezbahalarda etkin sanitasyon yöntemlerinin uygulanması ile gıda kaynaklı patojenlerin karkaslara bulaşmasının önlenmesi sağlanmalıdır.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ÇADIRCI Ö, gücükoğlu a, TERZİ GÜLEL G, UYANIK T, ALİŞARLI M (2018). The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. , 305 - 311. 10.1501/Vetfak_0000002861 |
| Chicago | ÇADIRCI Özgür,gücükoğlu ali,TERZİ GÜLEL GÖKNUR,UYANIK TOLGA,ALİŞARLI MUSTAFA The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. (2018): 305 - 311. 10.1501/Vetfak_0000002861 |
| MLA | ÇADIRCI Özgür,gücükoğlu ali,TERZİ GÜLEL GÖKNUR,UYANIK TOLGA,ALİŞARLI MUSTAFA The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. , 2018, ss.305 - 311. 10.1501/Vetfak_0000002861 |
| AMA | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. . 2018; 305 - 311. 10.1501/Vetfak_0000002861 |
| Vancouver | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. . 2018; 305 - 311. 10.1501/Vetfak_0000002861 |
| IEEE | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M "The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR." , ss.305 - 311, 2018. 10.1501/Vetfak_0000002861 |
| ISNAD | ÇADIRCI, Özgür vd. "The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR". (2018), 305-311. https://doi.org/10.1501/Vetfak_0000002861 |
| APA | ÇADIRCI Ö, gücükoğlu a, TERZİ GÜLEL G, UYANIK T, ALİŞARLI M (2018). The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65(3), 305 - 311. 10.1501/Vetfak_0000002861 |
| Chicago | ÇADIRCI Özgür,gücükoğlu ali,TERZİ GÜLEL GÖKNUR,UYANIK TOLGA,ALİŞARLI MUSTAFA The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi 65, no.3 (2018): 305 - 311. 10.1501/Vetfak_0000002861 |
| MLA | ÇADIRCI Özgür,gücükoğlu ali,TERZİ GÜLEL GÖKNUR,UYANIK TOLGA,ALİŞARLI MUSTAFA The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.65, no.3, 2018, ss.305 - 311. 10.1501/Vetfak_0000002861 |
| AMA | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(3): 305 - 311. 10.1501/Vetfak_0000002861 |
| Vancouver | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(3): 305 - 311. 10.1501/Vetfak_0000002861 |
| IEEE | ÇADIRCI Ö,gücükoğlu a,TERZİ GÜLEL G,UYANIK T,ALİŞARLI M "The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65, ss.305 - 311, 2018. 10.1501/Vetfak_0000002861 |
| ISNAD | ÇADIRCI, Özgür vd. "The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR". Ankara Üniversitesi Veteriner Fakültesi Dergisi 65/3 (2018), 305-311. https://doi.org/10.1501/Vetfak_0000002861 |
