Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses
Yıl: 2017 Cilt: 47 Sayı: 2 Sayfa Aralığı: 68 - 73 Metin Dili: İngilizce DOI: 10.4274/tjo.79059 İndeks Tarihi: 12-12-2018
Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses
Öz: Objectives: To compare biofilm formations of two Staphylococcus epidermidis (S. epidermidis) isolates with known biofilm formationcapacities on four different intraocular lenses (IOL) that have not been studied before.Materials and Methods: Two isolates obtained from ocular surfaces and identified in previous studies and stored at -86 °C in 15%glycerol in the microbiology laboratory of the Anadolu University Department of Biology were purified and used in the study. The isolateswere S. epidermidis KA 15.8 (ICA+), a known biofilm producer isolate positive for icaA, icaD and bap genes, and S. epidermidis KA 14.5 (ICA-),known as a non-biofilm producer isolate negative for icaA, icaD and bap genes. The biofilm formation capacities of the 2 isolates on 4 differentIOLs were compared. Two of the IOLs were acrylic (UD613 [IOL A], Turkey; SA60AT [IOL B], USA), and the other two were polymethylmethacrylate (PMMA) (B60130C [IOL C], India; B55125C [IOL D], India). Bacterial enumeration and optical density measurements weredone from biofilms that formed on the IOLs. Biofilms were imaged using scanning electron microscopy.Results: Mean bacterial counts on the IOLs were 7.1±0.4 log10 CFU/mL with the ICA+ isolate, and 6.7±0.8 log10 CFU/mL with theICA- isolate; there were no statistically significant differences. Biofilm formation was lower with acrylic lenses than PMMA lenses withboth isolates (p=0.009 and p=0.013). The highest biofilm production was obtained on IOL C (PMMA) (p<0.001) and the lowest wasobtained on IOL A (hydrophilic acrylic) (p<0.001).Conclusion: Bacterial counts after biofilm formation were lower on acrylic lenses, especially hydrophilic acrylic with hydrophobicproperties. Further animal and in vivo studies are required to support the findings of this study.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Huang XD, Yao K, Zhang H, Huang XJ, Xu ZK. Surface modification of silicone intraocular lens by 2-methacryloyloxyethyl phosphoryl-choline binding to reduce Staphylococcus epidermidis adherence. Clin Exp Ophthalmol. 2007;35:462-467.
- Cağavi F, Akalan N, Celik H, Gür D, Guçiz B. Effect of hydrophilic coating on microorganism colonization in silicone tubing. Acta Neurochir (Wien). 2004;146:603-610.
- Nomura S, Lundberg F, Stollenwerk M, Nakamura K, Ljungh A. Adhesion of staphylococci to polymers with and without immobilized heparin in cerebrospinal fluid. J Biomed Mater Res. 1997;38:35-42.
- Kıvanç SA, Kıvanç M, Bayramlar H. Microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. J Wound Care. 2016;25:14-19.
- Weston K, Nicholson R, Bunce C, Yang YF. An 8-year retrospective study of cataract surgery and postoperative endophthalmitis: injectable intraocular lenses may reduce the incidence of postoperative endophthalmitis. Br J Ophthalmol. 2015;99:1377-1380.
- Bausz M, Fodor E, Resch MD, Kristóf K. Bacterial contamination in the anterior chamber after povidone-iodine application and the effect of the lens implantation device. J Cataract Refract Surg. 2006;32:1691-1695.
- Vafidis GC, Marsh RJ, Stacey AR. Bacterial contamination of intraocular lens surgery. Br J Ophthalmol. 1984;68:520-523.
- Fazly Bazzaz BS, Jalalzadeh M, Sanati M, Zarei-Ghanavati S, Khameneh B. Biofilm Formation by Staphylococcus epidermidis on Foldable and Rigid Intraocular Lenses. Jundishapur J Microbiol. 2014;7:e10020.
- Baillif S, Ecochard R, Casoli E, Freney J, Burillon C, Kodjikian L. Adherence and kinetics of biofilm formation of Staphylococcus epidermidis to different types of intraocular lenses under dynamic flow conditions. J Cataract Refract Surg. 2008;34:153-158.
- Schroeder AC, Schmidbauer JM, Sobke A, Seitz B, Ruprecht KW, Herrmann M. Influence of fibronectin on the adherence of Staphylococcus epidermidis to coated and uncoated intraocular lenses. J Cataract Refract Surg. 2008;34:497-504.
- Prasad S, Nayak N, Satpathy G, Nag TC, Venkatesh P, Pandey RM. Biofilm: The Haven for Staphylococcus epidermidis in Post-operative Endophthalmitis. J Clin Exp Ophthalmol. 2014;5:1-5.
- Kodjikian L, Burillon C, Lina G, Roques C, Pellon G, Freney J, Renaud FN. Biofilm formation on intraocular lenses by a clinical strain encoding the ica locus: a scanning electron microscopy study. Invest Ophthalmol Vis Sci. 2003;44:4382-4387.
- Kivanc SA, Akova Budak B, Yildiz M, Kivanc M. The effect of the linezolid and the vancomycine on biofilm production that formed on two different acrylic hydrophobic intraocular lenses. Invest Ophthalmol Vis Sci. 2015;56:287.
- Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet. 2001;14;358:135-138.
- Arciola CR, Baldassarri L, Montanaro L. Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheterassociated infections. J Clin Microbiol. 2001;39:2151-2156.
- Pitts B, Hamilton MA, Zelver N, Stewart PS. A microtiter-plate screening method for biofilm disinfection and removal. J Microbiol Methods. 2003;54:269-276.
- Fey PD, Olson ME. Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol. 2010;5:917-933.
- García-Sáenz MC, Arias-Puente A, Fresnadillo-Martinez MJ, MatillaRodriguez A. In vitro adhesion of Staphylococcus epidermidis to intraocular lenses. J Cataract. Refract Surg. 2000;26:1673-1679.
- Kodjikian L, Burillon C, Roques C, Pellon G, Renaud FN, Hartmann D, Freney J. Intraocular lenses, bacterial adhesion and endophthalmitis prevention: a review. Biomed Mater Eng. 2004;14:395-409.
- Okajima Y, Kobayakawa S, Tsuji A, Tochikubo T. Biofilm formation by Staphylococcus epidermidis on intraocular lens material. Invest Ophthalmol Vis Sci. 2006;47:2971-2975.
- Zegans ME, Becker HI, Budzik J, O’Toole G. The role of bacterial biofilms in ocular infections. DNA Cell Biol. 2002;21:415-420.
- Bispo PJ, Haas W, Gilmore MS. Biofilms in infections of the eye. Pathogens. 2015;4:111-136.
- Ahmad S, Ashraf H, S.M. Akram SM. Adhesion of Biofilm forming Staphylococcus epidermidis strains on Intraocular Lenses – An Update. In: Méndez-Vilas A, ed. The Battle Against Microbial Pathogens: Basic Science, Technological Advances and Educational Programs. Formatex; 2015:357- 363.
- Schauersberger J, Amon M, Aichinger D, Georgopoulos A. Bacterial adhesion to rigid and foldable posterior chamber intraocular lenses: in vitro study. J Cataract Refract Surg. 2003;29:361-366.
- Speaker MG, Milch FA, Shah MK, Eisner W, Kreiswirth BN. Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis. Ophthalmology. 1991;98:639-649.
- Perkins RE, Kundsin RB, Pratt MV, Abrahamsen I, Leibowitz HM. Bacteriology of normal and infected conjunctiva. J Clin Microbiol. 1975;1:147-149.
- Esteban J, Pe´rez-Tanoira R, Pe´rez-Jorge-Peremarch C, Go´mez-Barrena E. Bacterial Adherence to Biomaterials Used in Surgical Procedures. In: Kon K, Rai M, eds. Microbiology for surgical infections Diagnosis, prognosis and treatment. Elsevier UK; 2014:41-59.
- Jabbarvand M, Hashemian H, Khodaparast M, Jouhari M, Tabatabaei A, Rezaei S. Endophthalmitis Occurring after Cataract Surgery: Outcomes of More Than 480 000 Cataract Surgeries, Epidemiologic Features, and Risk Factors. Ophthalmology. 2016;123:295-301.
- Miller JJ, Scott IU, Flynn HW Jr, Smiddy WE, Newton J, Miller D. Acuteonset endophthalmitis after cataract surgery (2000-2004): incidence, clinical settings, and visual acuity outcomes after treatment. Am J Ophthalmol. 2005;139:983-987.
- West ES, Behrens A, McDonnell PJ, Tielsch JM, Schein OD. The incidence of endophthalmitis after cataract surgery among the U.S. Medicare population increased between 1994 and 2001. Ophthalmol. 2005;112:1388-1394.
- Taban M, Behrens A, Newcomb RL, Nobe MY, Saedi G, Sweet PM, McDonnell PJ. Acute endophthalmitis following cataract surgery: a systematic review of the literature. Arch Ophthalmol. 2005;123:613-620.
- Wong TY, Chee SP. The epidemiology of acute endophthalmitis after cataract surgery in an Asian population. Ophthalmology. 2004;111:699-705.
| APA | Kıvanç S, KIVANÇ M, KILIÇ V, GÜLLÜLÜ G, ÖZMEN A (2017). Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. , 68 - 73. 10.4274/tjo.79059 |
| Chicago | Kıvanç Sertaç Argun,KIVANÇ Merih,KILIÇ VOLKAN,GÜLLÜLÜ Gülay,ÖZMEN Ahmet Tuncer Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. (2017): 68 - 73. 10.4274/tjo.79059 |
| MLA | Kıvanç Sertaç Argun,KIVANÇ Merih,KILIÇ VOLKAN,GÜLLÜLÜ Gülay,ÖZMEN Ahmet Tuncer Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. , 2017, ss.68 - 73. 10.4274/tjo.79059 |
| AMA | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. . 2017; 68 - 73. 10.4274/tjo.79059 |
| Vancouver | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. . 2017; 68 - 73. 10.4274/tjo.79059 |
| IEEE | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A "Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses." , ss.68 - 73, 2017. 10.4274/tjo.79059 |
| ISNAD | Kıvanç, Sertaç Argun vd. "Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses". (2017), 68-73. https://doi.org/10.4274/tjo.79059 |
| APA | Kıvanç S, KIVANÇ M, KILIÇ V, GÜLLÜLÜ G, ÖZMEN A (2017). Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi, 47(2), 68 - 73. 10.4274/tjo.79059 |
| Chicago | Kıvanç Sertaç Argun,KIVANÇ Merih,KILIÇ VOLKAN,GÜLLÜLÜ Gülay,ÖZMEN Ahmet Tuncer Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi 47, no.2 (2017): 68 - 73. 10.4274/tjo.79059 |
| MLA | Kıvanç Sertaç Argun,KIVANÇ Merih,KILIÇ VOLKAN,GÜLLÜLÜ Gülay,ÖZMEN Ahmet Tuncer Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi, vol.47, no.2, 2017, ss.68 - 73. 10.4274/tjo.79059 |
| AMA | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi. 2017; 47(2): 68 - 73. 10.4274/tjo.79059 |
| Vancouver | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses. Türk Oftalmoloji Dergisi. 2017; 47(2): 68 - 73. 10.4274/tjo.79059 |
| IEEE | Kıvanç S,KIVANÇ M,KILIÇ V,GÜLLÜLÜ G,ÖZMEN A "Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses." Türk Oftalmoloji Dergisi, 47, ss.68 - 73, 2017. 10.4274/tjo.79059 |
| ISNAD | Kıvanç, Sertaç Argun vd. "Comparison of Biofilm Formation Capacities of Two Clinical Isolates of Staphylococcus Epidermidis with and without icaA and icaD Genes on Intraocular Lenses". Türk Oftalmoloji Dergisi 47/2 (2017), 68-73. https://doi.org/10.4274/tjo.79059 |
