EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL

AKTAŞ, Serdar; ERKEN, HAYDAR ALİ; İSMAİLOĞLU, Özgür; Topcu, Abdullah; ABAS, BURÇİN İREM; ERSOY, Nevin; Yenisey, Çiğdem; kizilay, zahir

EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL

Zahir KIZILAY, (Adnan Menderes Üniversitesi Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Aydın, Türkiye)

Haydar Ali ERKEN, (Balıkesir Üniversitesi Tıp Fakültesi, Fizyoloji Anabilim Dalı, Balıkesir, Türkiye)

Serdar AKTAŞ, (Adnan Menderes Üniversitesi Tıp Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Aydın, Türkiye)

Nevin ERSOY, (Dokuz Eylül Üniversitesi Tıp Fakültesi, Histoloji-Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Burçin İrem ABAS, (Adnan Menderes Üniversitesi Tıp Fakültesi, Klinik Biyokimya Anabilim Dalı, Aydın, Türkiye)

Abdullah TOPÇU, (Adnan Menderes Üniversitesi Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Aydın, Türkiye)

Çiğdem YENİSEY, (Adnan Menderes Üniversitesi Tıp Fakültesi, Klinik Biyokimya Anabilim Dalı, Aydın, Türkiye)

Özgür İSMAİLOĞLU (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Isparta, Türkiye)

İstanbul Tıp Fakültesi Dergisi

3 0

Yıl: 2017 Cilt: 80 Sayı: 4 Sayfa Aralığı: 138 - 145 Metin Dili: İngilizce İndeks Tarihi: 14-12-2018

EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL

Öz:

Objective: Although, the neuroprotective effects of selenium are known, its effect on peripheral nerve injury is not clear. Thestudy was aimed to investigate whether selenium prevents axonal and myelin damage in experimental sciatic nerve injury.Materials and Methods: Twenty-eight male Wistar albino rats were divided into four groups (n=7 in each): control (C),selenium (S), injury (I), and selenium-treated injury (SI). Injury was generated by 30 second of compression via Yasargilaneurysm clip on the sciatic nerve of rats in the I and SI groups. Then, selenium was given to the S and SI groups as 1.5 mg/kg by oral gavage at 1st, 24th, 48th and 72nd hour after surgery. According to the experimental protocol, electrophysiological,histological, and biochemical tests were performed end of the day 4.Results: Whereas the amplitude of compound action potential, nerve conduction velocity, average axon diameter, myelin thickness,myelinated/unmyelinated axons and SOD activity in red blood cells of the I group were significantly lower than those ofthe C, S and SI groups, the serum MDA levels of the I group were significantly higher than those of the C, S and SI groups.Conclusion: The findings of this study show that selenium decreases axonal and myelin damage after sciatic nerve injuryand that this neuroprotective effect of selenium is at least partially mediated by oxidant/antioxidant mechanisms.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

DENEYSEL SİYATİK SİNİR HASARINDA SELENYUMUN AKSON VE MİYELİN İYİLEŞMESİ ÜZERİNE ETKİLERİ

Öz:

Amaç: Selenyumun nöroprotektif etkileri bilinmesine rağmen, periferik sinir yaralanmasında etkisi açık değildir. Bu çalışmada amacımız, deneysel siyatik sinir hasarında selenyumun akson ve miyelin yaralanmasına koruyucu etkisi olup olmadığını araştırmaktı. Gereç ve Yöntem: Yirmi sekiz adet wistar albino cinsi rat her grupta 7 rat olacak şekilde 4 eşit gruba rastlantısal olarak ayrıldı. Gruplar kontrol, selenyum, yaralanma ve selenyum ile tedavi edilen yaralanma gruplarından oluşturuldu. Yaralanma Yaşargil anevrizma klibi ile 30 saniye süreyle siyatik sinire bası oluşturularak yaralanma ve selenyumla tedavi edilen yaralanma gruplarına uygulandı. Selenyum, 1,5 mg/kg’dan oral olarak selenyum ve selenyumla tedavi edilen gruplara cerrahiden sonra birinci, yirmi dördüncü, kırk sekizinci ve yetmiş ikinci saatte verildi. Deneysel prosedüre göre dördüncü günün bitiminde elektrofizyolojik, histolojik ve biyokimyasal testler yapıldı. Bulgular: Birleşik aksiyon potansiyeli amplitüdü, sinir ileti hızı, ortalama akson çapı, miyelin kalınlığı, miyelinli ve miyelinsiz akson sayısı ve eritrositlerdeki SOD aktivitesi yaralanma grubunda kontrol, selenyum ve selenyumla tedavi edilen gruplardan belirgin olarak düşük olmasına karşın serum MDA düzeyi yaralanma grubunda diğer gruplara nazaran daha yüksekti. Sonuç: Çalışmanın bu bulguları, selenyumun siyatik sinir yaralanmasından sonra akson ve miyelin hasarını azalttığını göstermiştir. Selenyumun bu nöroprotektif etkisine en azından kısmi olarak oksidan/antioksidan mekanizmalar aracılık etmiştir

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Zochodne DW, Levy D. Nitric oxide in damage, disease and repair of the peripheral nervous system. Cell Moll Biol (Noisy-le-grand). 2005;51(3):255-67.
Ma J, Liu J, Wang Q, Yu H, Chen Y, Xiang L. The benefical effect of ginsenoside Rg1on Schwann cells subjected to hydrogen peroxide induced oxidative injury. Int J Biol Sci 2013;9(6):624-36. [CrossRef]
Luo X, Chen B, Zheng R, Lin P, Li J, Chen H. Hydrogen peroxide induces apoptosis through the mitochondrial pathway in rat Schwann cells. Neurosci Lett 2010;485(1):60-4. [CrossRef]
Ide C. Peripheral nerve regeneration. Neurosci Res 1996;25(2):101-21. [CrossRef]
Huang HC, Chen L, Zhang HX, Li SF, Liu P, Zhao TY, et al. Autophagy promotes peripheral nerve regeneration and motor recovery following sciatic nerve crush injury in rats. J Mol Neurosci 2016;58(4):416-23. [CrossRef]
Clarke D, Richardson P. Peripheral nerve injury. Curr Opin Neurol 1994;7(5):415-21. [CrossRef]
Bowe CM, Hildebrand C, Kocsis JD, Waxman SG. Morphological and physiological properties of neurons after long-term axonal regeneration: observations on chronic and delayed sequelae of peripheral nerve injury. J Neurol Sci 1989;91(3):259-92. [CrossRef]
Turkoglu E, Serbes G, Dolgun H, Oztuna S, Bagdatoglu OT, Yilmaz N, et al. Effects of α-MSH on ischemia /reperfusion injury in rat sciatic nerve. Surg Neurol Int 2012;3:74. [CrossRef]
Ye N, Liu S, Lin Y, Rao P. Protective effects of intraperitoneal injection of TAT-SOD against focal cerebral ischemia/reperfusion injury in rats. Life Sci 2011;89(23-24):868-74. [CrossRef]
Zhan T, Li Z, Dong J, Nan F, Li T, Yu Q. Edaravone promotes functional recovery after mechanical peripheral nerve injury. Neural Regen Res 2014;9(18):1709- 15. [CrossRef]
Yang TC, Chen YJ, Chan SF, Chen CH, Chang PY, Lu SC. Malondialdehyde mediates oxidized LDL-induced coronary toxicity through the Akt-FGF2 pathway via DNA methylation. J Biomed Sci 2014;21:11. [CrossRef]
Uğuz AC, Naziroğlu M, Espino J, Bejarano I, Gonzalez D, Rodriquez AB, et al. Selenium modulates oxidative stress-induced cell apoptosis in human myeloid HL-60 cells through regulation of calcium release and caspase-3 and -9 activities. J Membr Biol 2009;232(1- 3):15-23. [CrossRef]
Savas S, Briollais L, Ibrahim-zada I, Jarjanazi H, Choi YH, Musguera M, et al. A whole-genome SNP association study of NCI60 cell line panel indicates a role of Ca2+ signaling in selenium resistance. PLoS One 2010;5(9):e12601. [CrossRef]
McKenzie RC, Arthur JR, Beckett GJ. Selenium and the regulation of cell signalling, growth and survival: molecular and mechanistic aspects. Antioxid Redox Signal 2002;4(2):339-51. [CrossRef]
Ayaz M, Kaptan H. Effect of selenium on electrophysiological changes associated with diabetic peripheral neuropathy. Neural Regen Res 2011;6:1.
Sarikcioglu L, Demir N, Demirtop A. A standardized method to create optic nerve crush: Yasargil aneurysm clip. Exp Eye Res 2007;84(2):373-7. [CrossRef]
Kato N, Nemoto K, Kawaguchi M, Amako M, Arino H, Fujikawa K. Influence of chronic inflammation in peripheral target tissue on recovery of crushed nerve injury. J Orthop Sci 2001;6(5):419-23. [CrossRef]
Chen LE, Seaber AV, Glisson RR, Davies H, Murrell GA, Anthony DC, et al. The functional recovery of peripheral nerves following defined acute crush injuries. J Orthop Res 1992;10(5):657-64. [CrossRef]
Raydevik B, Lundborg G, Bagge U. Effect of graded compression on intraneural blood flow. An in vivo study on rabbit tibial nerve. J Hand Surg Am 1981;6(1):3-12. [CrossRef]
De Koning P, Brakkee JH, Gispen WH. Methods for production reproducible crush in the sciatic and tibial nerve of the rat and rapid and precise testin of return of sensory function: beneficial effects of melanocortins. J Neurol Sci 1986;74(2):237-46. [CrossRef]
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988;34(3):497-500.
Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990;186:407- 21. [CrossRef]
Kızılay Z, Erken HA, Çetin NK, Aktaş S, Abas Bİ, Yılmaz A. Boric acid reduces axonal and myelin damage in experimental sciatic nerve injury. Neural Regen Res 2016;11(10):1660-5. [CrossRef]
Erken HA, Koç ER, Yazıcı H, Yay A, Önder GÖ, Sarıcı SF. Selenium partially prevents cisplatin-induced neurotoxicity: a preliminary study. Neurotoxicology 2014;42:71-5. [CrossRef]
Godoi GL, de Oliveira Ponciuncula L, Schultz JF, Kaufmann FN, da Rocha JB, de Souza DO, et al. Selenium compounds prevent amyloid β-peptide neurotoxicity in rat primary hippocampal neurons. Neurochem Res 2013;38(11):2359-63. [CrossRef]
Ben Amara I, Fetoui H, Guermazi F, Zeghal N. Dietary selenium addition improves cerebrum and cerebellumimpairments induced by methimazole in suckling rats. Int J Dev Neurosci 2009;27(7):719-26. [CrossRef]
Ahmad A, Khan MM, Ishrat T, Khan MB, Khuwaja G, Raza SS, et al. Synergistic effect of selenium and melatonin on neuroprotection in cerebral ischemia in rats. Biol Trace Elem Res 2011;139(1):81-96. [CrossRef]
Ansar S. Effect of selenium on the levels of cytokines and trace elements in toxin-mediated oxidative stress in male rats. Biol Trace Elem Res 2016;169(1):129- 33. [CrossRef]
Chen XB, Yuan H, Wang FJ, Tan ZX, Liu H, Chen N. Protective role of selenium-enriched supplement on spinal cord injury through the upregulation of CNTF and CNTF-Ralpha. Eur Rev Med Pharmacol Sci 2015;19(22):4434-42.
Wirth EK, Conrad M, Winterer J, Wozny C, Carlson BA, Roth S, et al. Neuronal selenoprotein expression is required for interneuron development and prevents seizures and neurodegeneration. FASEB J 2010;24(3):844-52. [CrossRef]
Nazıroğlu M, Şenol N, Ghazizadeh V, Yürüker V. Neuroprotection induced by N-acetylcysteine and selenium againts traumatic brain injury-induced apoptosis and calcium entry in hippocampus of rats. Cell Mol Neurobiol 2014;34(6):895-903. [CrossRef]
Uğuz AC, Nazıroğlu M. Effect of selenium on calcium signaling and apoptosis in rat dorsal root ganglion neurons induced by oxidative stress. Neurochem Res 2012;37(8):1631-8. [CrossRef]
Ince S, Kucukkurt I, Cigerci IH, Fatih Fidan A, Eryavuz A. The effect of dietary boric acid and borax supplementation on lipid peroxidation, antioxidant activity, and DNA damage in rats. J Trace Elem Med Biol 2010;24(3):161-4. [CrossRef]
Pan HC, Sheu ML, Su HL, Chen YJ, Chen CJ, Yang DY, et al. Magnesium supplement promotes sciatic nerve regeneration and down-regulates inflammatory response. Magnes Res 2011;24(2):54-70.
Karalija A, Novikova LN, Kingham PJ, Wiberg M, Novikov LN. Neuroprotective effects of N-acetylcysteine and acetyl-L-carnitine after spinal cord injury in rats. PLoS One 2012;7(7):e41086. [CrossRef]
Sarikcioglu L, Ozkan O. Yasargil-Phynox aneurysm clip: a simple and reliable device for making a peripheral nerve injury. Int J Neurosci 2003;113(4):455-64. [CrossRef]
Geuna S. The sciatic nerve injury model in pre-clinical research. J Neurosci Methods 2015;243:39-46. [CrossRef]
Mattson MP, Camandola S. NF-κB in neuronal plasticity and neurodegenerative disorders. J Clin Invest 2001;107(3):247-54. [CrossRef]
Barnes PJ, Karin M. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. New Engl J Med 1997;336(15):1066-71. [CrossRef]

APA	kizilay z, ERKEN H, AKTAŞ S, ERSOY N, ABAS B, Topcu A, Yenisey Ç, İSMAİLOĞLU Ö (2017). EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. , 138 - 145.
Chicago	kizilay zahir,ERKEN HAYDAR ALİ,AKTAŞ Serdar,ERSOY Nevin,ABAS BURÇİN İREM,Topcu Abdullah,Yenisey Çiğdem,İSMAİLOĞLU Özgür EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. (2017): 138 - 145.
MLA	kizilay zahir,ERKEN HAYDAR ALİ,AKTAŞ Serdar,ERSOY Nevin,ABAS BURÇİN İREM,Topcu Abdullah,Yenisey Çiğdem,İSMAİLOĞLU Özgür EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. , 2017, ss.138 - 145.
AMA	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. . 2017; 138 - 145.
Vancouver	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. . 2017; 138 - 145.
IEEE	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö "EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL." , ss.138 - 145, 2017.
ISNAD	kizilay, zahir vd. "EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL". (2017), 138-145.

APA	kizilay z, ERKEN H, AKTAŞ S, ERSOY N, ABAS B, Topcu A, Yenisey Ç, İSMAİLOĞLU Ö (2017). EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. İstanbul Tıp Fakültesi Dergisi, 80(4), 138 - 145.
Chicago	kizilay zahir,ERKEN HAYDAR ALİ,AKTAŞ Serdar,ERSOY Nevin,ABAS BURÇİN İREM,Topcu Abdullah,Yenisey Çiğdem,İSMAİLOĞLU Özgür EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. İstanbul Tıp Fakültesi Dergisi 80, no.4 (2017): 138 - 145.
MLA	kizilay zahir,ERKEN HAYDAR ALİ,AKTAŞ Serdar,ERSOY Nevin,ABAS BURÇİN İREM,Topcu Abdullah,Yenisey Çiğdem,İSMAİLOĞLU Özgür EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. İstanbul Tıp Fakültesi Dergisi, vol.80, no.4, 2017, ss.138 - 145.
AMA	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. İstanbul Tıp Fakültesi Dergisi. 2017; 80(4): 138 - 145.
Vancouver	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL. İstanbul Tıp Fakültesi Dergisi. 2017; 80(4): 138 - 145.
IEEE	kizilay z,ERKEN H,AKTAŞ S,ERSOY N,ABAS B,Topcu A,Yenisey Ç,İSMAİLOĞLU Ö "EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL." İstanbul Tıp Fakültesi Dergisi, 80, ss.138 - 145, 2017.
ISNAD	kizilay, zahir vd. "EFFECTS OF SELENIUM ON AXON AND MYELIN HEALING IN AN EXPERIMENTAL SCIATIC NERVE INJURY MODEL". İstanbul Tıp Fakültesi Dergisi 80/4 (2017), 138-145.