Diversity of microfungi in acid mine drainages

ÇABUK, AHMET; KOCABIYIK, Yaşar Erçin; GEDIKLI, Serap; YENİCE GÜRSU, BÜKAY; İlhan, Semra; Aytar Çelik, Pınar

Diversity of microfungi in acid mine drainages

Bükay Yenice GÜRSU, (Eskisehir Osmangazi University; Central Research Laboratory Application and Research Center (ARUM) 26480, Odunpazari, Eskisehir,Turkey)

PINAR AYTAR ÇELİK, (Department of Biotechnology and Biosafety, Graduate School of Natural and Applied Sciences, Eskişehir Osmangazi University, 26480, Eskişehir, Turkey)

Semra İLHAN, (Department of Biology, Faculty of Arts and Science, Eskişehir Osmangazi University, 26480 Eskişehir, Turkey)

Yaşar Erçin KOCABIYIK, (Graduate School of Natural and Applied Sciences, Eskişehir Osmangazi University, 26480 Eskişehir, Turkey)

Serap GEDIKLI, (Graduate School of Natural and Applied Sciences, Eskişehir Osmangazi University, 26480 Eskişehir, Turkey)

Ahmet ÇABUK (Department of Biology, Faculty of Arts and Science, Eskişehir Osmangazi University, 26480 Eskişehir, Turkey)

Biological Diversity and Conservation

4 0

Yıl: 2017 Cilt: 10 Sayı: 3 Sayfa Aralığı: 190 - 198 Metin Dili: İngilizce İndeks Tarihi: 14-12-2018

Diversity of microfungi in acid mine drainages

Öz:

Acid mine drainage (AMD) is an anthropogenic system having lower acidic pH value and high specific conductivity. In this study, from two different acidic environments, the AMDs of Çan (Çanakkale) (pH 2.85) and of Balya (Balıkesir) (pH 2.75) the microfungi was isolated and identification. Morphological and molecular techniques were used to identify microfungi. Then fifteen species belonging to nine genera were identified. They are listed as follows: Aspergillus awamori, A. repens, Cladosporium herbarum, C. oxysporum, Penicillium citrinum, P. montanense, P. ochrochloron, P. spinulosum, P. verrucosum, Penidiella sp., Phialophora sp., Talaromyces aculeatus, T. helicus, Trichoderma harzianum, ve Umbelopsis autotrophica. Most of these species have been commonly found in acidic and/or heavy metal-rich environments. Consequently, such isolates from AMDs, which represents an extreme environment, are important because of revealing the fungal diversity and their potential biotechnological applications.

Anahtar Kelime:

Asit maden drenajlarında mikrofungus çeşitliliği

Öz:

Asit maden drenajı (AMD), daha düşük asidik pH değerine ve yüksek spesifik iletkenliğe sahip olan bir antropojenik sistemdir. Bu çalışmada, Çanakkale (pH 2.85) ve Balya (Balıkesir) (pH 2.75) AMD'leri olmak üzere iki farklı asidik ortamdan mikrofunguslar izole edildi ve tanılandı. Asidik maden drenaj sularından izole edilen mikrofungusları belirlemek için morfolojik ve moleküler teknikler kullanılmış ve listelendiği gibi dokuz cinse ait on beş tür tespit edilmiştir; Aspergillus awamori, A. repens, Cladosporium herbarum, C. oxysporum, Penicillium citrinum, P. montanense, P. ochrochloron, P. spinulosum, P. verrucosum, Penidiella sp., Phialophora sp., Talaromyces aculeatus, T. helicus, Trichoderma harzianum, ve Umbelopsis autotrophica. Bu türlerin çoğu asidik ve/veya ağır metal açısından zengin ortamlarda bulunur. Sonuç olarak, ekstrem çevreyi temsil eden AMD'lerinden elde edilen bu izolatlar bu ortamlardaki fungus çeşitliliğinin ortaya çıkarılması ve potansiyel biyoteknolojik uygulamaları nedeniyle önemlidir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Zhang, F., Shi, P., Bai, Y., Luo, H., Yuan, T., Huang, H., Yao, B. (2011). An acid and highly thermostable xylanase from Phialophora sp. G5. Applied Microbiology and Biotechnology, 89(6), 1851-1858.
Yucel, D. S., Yucel, M. A., and Baba, A. (2014). Change detection and visualization of acid mine lakes using time series satellite image data in geographic information systems (GIS): Can (Canakkale) County, NW Turkey. Environmental Earth Sciences, 72(11), 4311-4323.
Watson, e. (1994). Official and Standardized Methods of Analysis: Royal Society of Chemistry.
Stokes, P., and Lindsay, J. (1979). Copper tolerance and accumulation in Penicillium ochro-chloron isolated from copperplating solution. Mycologia, 796-806.
Starkey, R., and Waksman, S. (1943). Fungi tolerant to extreme acidity and high concentrations of copper sulfate. Journal of Bacteriology, 45(5), 509-519.
Sonjak, S., Frisvad, J. C., and Gunde-Cimerman, N. (2006). Penicillium mycobiota in arctic subglacial ice. Microb Ecol, 52(2), 207-216. doi:10.1007/s00248-006-9086-0
Samson, R. A., Hoekstra, E. S., and Frisvad, J. C. (2004). Introduction to food-and airborne fungi: Centraalbureau voor Schimmelcultures (CBS).
Rawlings, D. E., and Johnson, D. B. (2007). The microbiology of biomining: development and optimization of mineraloxidizing microbial consortia. Microbiology, 153(2), 315-324.
Raper, K., and Thom, C. (1949). A manual of the Penicillia. 875 pp. Williams and Wilkins, Baltimore: Maryland, USA.
Prasad, M. N. V., and Jeeva, S. (2009). Coal mining and its leachate are potential threats to Nepenthes khasiana Hook. f.(Nepenthaceae) that preys on insects-an endemic plant in North Eastern India. Biol Div Con, 2, 29-33.
Pitt, J., and Hocking, A. (2009). Fungi and food spoilage: Springer Verlag.
Petrovic, U., Gunde-Cimerman, N., and Zalar, P. (2000). Xerotolerant mycobiota from high altitude Anapurna soil, Nepal. FEMS Microbiol Lett, 182(2), 339-342. doi:S0378-1097(99)00614-X [pii]
Mueller, G. M., and Schmit, J. P. (2007). Fungal biodiversity: what do we know? What can we predict? Biodiversity and Conservation, 16(1), 1-5.
Méndez-García, C., Peláez, A. I., Mesa, V., Sánchez, J., Golyshina, O. V., and Ferrer, M. (2015). Microbial diversity and metabolic networks in acid mine drainage habitats. Frontiers in microbiology, 6.
Klich, M. (2002). Identification of common Aspergillus species. 122 pp. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands.
Hujslová, M., Kubátová, A., Chudíčková, M., and Kolařík, M. (2010). Diversity of fungal communities in saline and acidic soils in the Soos National Natural Reserve, Czech Republic. Mycological Progress, 9(1), 1-15.
He, Y., Li, J., Wang, J., Rao, J., and Mao, H. (2011). Separation and purification of β-glucosidase from Penidiella sp. HEY-1 and its properties. Chemistry and Industry of Forest Products, 31(3), 110-114.
Hawksworth, D. L. (2001). The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycological Research, 105(12), 1422-1432.
Gross, S., and Robbins, E. I. (2000). Acidophilic and acid-tolerant fungi and yeasts. Hydrobiologia, 433(1-3), 91-109.
Graham, G., Mayers, P., and Henry, R. (1994). A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques, 16(1), 48.
Gould, W., Fujikawa, J., and Cook, F. (1974). A soil fungus tolerant to extreme acidity and high salt concentrations. Canadian Journal of Microbiology, 20(7), 1023-1027.
Fukami, M., Yamazaki, S., and Toda, S. (1983). Distribution of copper in the cells of heavy metal tolerant fungus, Penicillium ochrochloron, cultured in concentrated copper medium. Agricultural and biological chemistry, 47(6), 1367-1369.
Domsch, K. H., Gams, W., and Anderson, T. H. (1993). Compendium of soil fungi (Vol. 1): Eching, Germany: IHW-Verlag. Ellis, M. (1971). Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew. Links.
Das, B., Roy, A., Singh, S., and Bhattacharya, J. (2009). Eukaryotes in acidic mine drainage environments: potential applications in bioremediation. Reviews in Environmental Science and Bio/Technology, 8(3), 257-274. doi:10.1007/s11157-009-9161-3
Çabuk, A., Aytar, P., Gedikli, S., Özel, Y., and Kocabıyık, E. (2013). Biosorption of acidic textile dyestuffs from aqueous solution by Paecilomyces sp. isolated from acidic mine drainage. Environmental Science and Pollution Research, 20(7), 4540-4550. doi:10.1007/s11356-012-1396-9
Cooke, W. B. (1976). Fungi in and near streams carrying acid mine-drainage.
Brake, S., Hasiotis, S., Dannelly, H., and Connors, K. (2002). Eukaryotic stromatolite builders in acid mine drainage: Implications for Precambrian iron formations and oxygenation of the atmosphere? Geology, 30(7), 599-602.
Barnett, H., and Hunter, B. (1999). Illustrated genera of imperfect fungi (p. 218). St. Paul: APS.
Baker, B. J., Lutz, M. A., Dawson, S. C., Bond, P. L., and Banfield, J. F. (2004). Metabolically active eukaryotic communities in extremely acidic mine drainage. Applied and Environmental Microbiology, 70(10), 6264-6271.
Baker, B. J., and Banfield, J. F. (2003). Microbial communities in acid mine drainage. FEMS microbiology ecology, 44(2), 139-152.
Baba, A., Gurdal, G., Sengunalp, F., and Ozay, O. (2008). Effects of leachant temperature and pH on leachability of metals from fly ash. A case study: Can thermal power plant, province of Canakkale, Turkey. Environmental Monitoring and Assessment, 139(1-3), 287-298. doi:10.1007/s10661-007-9834-8
Bååth, E., Lundgren, B., and Söderström, B. (1984). Fungal populations in podzolic soil experimentally acidified to simulate acid rain. Microbial ecology, 10(3), 197-203.
Aytar, P., Kay, C. M., Mutlu, M. B., and Çabuk, A. (2013). Coal Desulphurization with Acidithiobacillus ferrivorans, from Balya Acidic Mine Drainage. Energy and Fuels.
Auld, R. R., Myre, M., Mykytczuk, N. C., Leduc, L. G., and Merritt, T. J. (2013). Characterization of the microbial acid mine drainage microbial community using culturing and direct sequencing techniques. Journal of Microbiological Methods, 93(2), 108-115.
Asan, A. (2004). Aspergillus, Penicillium and related species reported from Turkey. Mycotaxon, 89(1), 155-157.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool. Journal of molecular biology, 215(3), 403-410.
Akyol, Z. (2012). Balıkesir-Balya cevherli sahalarının jeolojisi, mineralojisi ve maden potansiyelinin değerlendirilmesi. İstanbul Yerbilimleri Dergisi, 3(1-2).

APA	YENİCE GÜRSU B, Aytar Çelik P, İlhan S, KOCABIYIK Y, GEDIKLI S, ÇABUK A (2017). Diversity of microfungi in acid mine drainages. , 190 - 198.
Chicago	YENİCE GÜRSU BÜKAY,Aytar Çelik Pınar,İlhan Semra,KOCABIYIK Yaşar Erçin,GEDIKLI Serap,ÇABUK AHMET Diversity of microfungi in acid mine drainages. (2017): 190 - 198.
MLA	YENİCE GÜRSU BÜKAY,Aytar Çelik Pınar,İlhan Semra,KOCABIYIK Yaşar Erçin,GEDIKLI Serap,ÇABUK AHMET Diversity of microfungi in acid mine drainages. , 2017, ss.190 - 198.
AMA	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A Diversity of microfungi in acid mine drainages. . 2017; 190 - 198.
Vancouver	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A Diversity of microfungi in acid mine drainages. . 2017; 190 - 198.
IEEE	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A "Diversity of microfungi in acid mine drainages." , ss.190 - 198, 2017.
ISNAD	YENİCE GÜRSU, BÜKAY vd. "Diversity of microfungi in acid mine drainages". (2017), 190-198.

APA	YENİCE GÜRSU B, Aytar Çelik P, İlhan S, KOCABIYIK Y, GEDIKLI S, ÇABUK A (2017). Diversity of microfungi in acid mine drainages. Biological Diversity and Conservation, 10(3), 190 - 198.
Chicago	YENİCE GÜRSU BÜKAY,Aytar Çelik Pınar,İlhan Semra,KOCABIYIK Yaşar Erçin,GEDIKLI Serap,ÇABUK AHMET Diversity of microfungi in acid mine drainages. Biological Diversity and Conservation 10, no.3 (2017): 190 - 198.
MLA	YENİCE GÜRSU BÜKAY,Aytar Çelik Pınar,İlhan Semra,KOCABIYIK Yaşar Erçin,GEDIKLI Serap,ÇABUK AHMET Diversity of microfungi in acid mine drainages. Biological Diversity and Conservation, vol.10, no.3, 2017, ss.190 - 198.
AMA	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A Diversity of microfungi in acid mine drainages. Biological Diversity and Conservation. 2017; 10(3): 190 - 198.
Vancouver	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A Diversity of microfungi in acid mine drainages. Biological Diversity and Conservation. 2017; 10(3): 190 - 198.
IEEE	YENİCE GÜRSU B,Aytar Çelik P,İlhan S,KOCABIYIK Y,GEDIKLI S,ÇABUK A "Diversity of microfungi in acid mine drainages." Biological Diversity and Conservation, 10, ss.190 - 198, 2017.
ISNAD	YENİCE GÜRSU, BÜKAY vd. "Diversity of microfungi in acid mine drainages". Biological Diversity and Conservation 10/3 (2017), 190-198.