KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ
Yıl: 2018 Cilt: 19 Sayı: 3 Sayfa Aralığı: 98 - 108 Metin Dili: Türkçe İndeks Tarihi: 20-05-2019
KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ
Öz: AMAÇ: Kadmiyuma (Cd) çevresel maruziyet hiperglisemive azalmış serum insülini ile ilişkilidir. Bu çalışma, Cd’yemaruz kalmış diyabetik ratlarda Lipoik Asit (LA) veinsülinin glikolitik enzimler, karaciğer marker enzimlerive lipidler üzerindeki etkilerini değerlendirmek içintasarlanmıştır.GEREÇ VE YÖNTEM: Erkek Wistar sıçanları 7 grubaayrıldı (n = 8). Gruplar kontrol, diyabetik kontrol, diyabet+ CdCl2, diyabet + insülin, diyabet + CdCl2 + insülin,diyabet + CdCl2 + LA ve diyabet + CdCl2 + insülin + LAgruplarından oluştu. Tip 1 diyabet, 6 gruba intraperitoneal(i.p.) streptozotosin (STZ) (65 mg / kg) enjeksiyonu ileindüklendi. İnsülin (4 IU/kg/gün), insülin ile tedavi edilengruplara subkutan (s.c.) verildi. CdCl2 (1,2 mg/kg/gün),CdCl2 ile tedavi edilen gruplara s.c. verildi. LA (100 mg/kg/gün), LA ile tedavi edilen gruplara i.p. verildi. CdCl2 veinsülin tedavisi, intraperitoneal STZ enjeksiyonundan 2gün sonra başlatıldı ve 3 hafta sürdürüldü. Serum glukoz,AST, ALT, ALP, BUN, LDL, HDL ve TG düzeyleri, tam kanHbA1c düzeyi ve karaciğer heksokinaz (HK), piruvat kinaz(PK) ve Na+/K+ ATPaz aktivitesi değerlendirildi.BULGULAR: Diyabetik grupta serum glukoz, HbA1c, TG,LDL, AST, ALT, ALP ve BUN düzeyleri kontrol grubunagöre daha yüksek bulundu, ancak HDL daha düşüktü.Diyabetik kontrol grubu karaciğer dokusunda Na+/K+ATPaz, HK ve PK aktivitesi azaldı. Diabetik + CdCl2 veDiabetik+İnsulin+CdCl2 gruplarında karaciğerde PK, HKve Na+/K+ ATPaz aktivitesi arttı. İnsülin ve LA ile tedaviedilen gruplarda HK, PK ve Na+/K+ ATPaz aktivitelerindediyabetik kontrol grubu ile karşılaştırıldığında artışsaptandı.SONUÇ: Bu sonuçlar insülin ve LA uygulamasının Cd veSTZ’nin neden olduğu karaciğer hasarına karşı etkili birterapötik müdahale olabileceğini düşündürmektedir.
Anahtar Kelime: Konular:
POSSIBLE REGULATORY EFFECTS OF APPLICATION OF ALPHA LIPOIC ACID AND INSULIN AGAINST CADMIUM EXPOSED DIABETIC RATS
Öz: OBJECTIVE: Environmental exposure to the cadmium (Cd), is associated with hyperglycemia and reduced serum insulin. This investigation was planned to assess the effects of Lipoic Acid (LA) and insulin on glycolytic enzymes, liver marker enzymes and lipids in Cd exposed diabetic rats. MATERIAL AND METHODS: Male Wistar rats were separated into 7 groups (n=8 in each group). Groups were designed as control, diabetic control, diabetic + CdCl2, diabetic + insulin, diabetic + CdCl2 + insulin, diabetic + CdCl2 + LA, anddiabetic + CdCl2 + insulin + LA groups. Type 1 diabetes was established by intraperitoneal (i.p.) injection of streptozotocin (STZ) (65 mg/kg) into 6 groups. Insulin (4 IU/kg/day) was given subcutaneously (s.c.) to insulin treated groups. CdCl2 (1,2 mg/kg/day) was given s.c. to CdCl2 treated groups. LA (100 mg/kg/day) was given i.p. to LA treated groups. CdCl2, LA, and insulin treatment were started 2 days after intraperitoneal STZ injection and continued for 3 weeks. Serum glucose, AST, ALT, BUN, LDL, HDL, and TG levels and liver hexokinase (HK), pyruvate kinase (PK), whole blood HbA1c level, and Na+/K+ATPase activity were evaluated. RESULTS: In diabetic group, serum glucose, HbA1c, TG, LDL, AST, ALT, ALP, and BUN levels were higher than cont- rol, but HDL was lower. In liver tissue, activities of Na+/ K+ATPase, HK and PK activities were decreased in dia- betic control group. PK, HK and Na+/K+ATPase activities were increased in liver in diabetic+CdCl2 and Diabeti- c+Insulin+CdCl2 groups. An increase was determined in activities of HK, PK, and Na+/K+ATPase in insulin and LA treated groups compared with diabetic control group. CONCLUSIONS: These results suggest that application of insulin and LA could be an effective therapeutic intervention against liver injury caused by Cd and STZ.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KILIÇ N, AKYÜZ F, Tekin Karacaer N (2018). KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. , 98 - 108. |
| Chicago | KILIÇ Neşe,AKYÜZ FAHRETTIN,Tekin Karacaer Neslihan KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. (2018): 98 - 108. |
| MLA | KILIÇ Neşe,AKYÜZ FAHRETTIN,Tekin Karacaer Neslihan KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. , 2018, ss.98 - 108. |
| AMA | KILIÇ N,AKYÜZ F,Tekin Karacaer N KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. . 2018; 98 - 108. |
| Vancouver | KILIÇ N,AKYÜZ F,Tekin Karacaer N KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. . 2018; 98 - 108. |
| IEEE | KILIÇ N,AKYÜZ F,Tekin Karacaer N "KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ." , ss.98 - 108, 2018. |
| ISNAD | KILIÇ, Neşe vd. "KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ". (2018), 98-108. |
| APA | KILIÇ N, AKYÜZ F, Tekin Karacaer N (2018). KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 19(3), 98 - 108. |
| Chicago | KILIÇ Neşe,AKYÜZ FAHRETTIN,Tekin Karacaer Neslihan KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 19, no.3 (2018): 98 - 108. |
| MLA | KILIÇ Neşe,AKYÜZ FAHRETTIN,Tekin Karacaer Neslihan KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, vol.19, no.3, 2018, ss.98 - 108. |
| AMA | KILIÇ N,AKYÜZ F,Tekin Karacaer N KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2018; 19(3): 98 - 108. |
| Vancouver | KILIÇ N,AKYÜZ F,Tekin Karacaer N KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ. Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi. 2018; 19(3): 98 - 108. |
| IEEE | KILIÇ N,AKYÜZ F,Tekin Karacaer N "KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ." Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi, 19, ss.98 - 108, 2018. |
| ISNAD | KILIÇ, Neşe vd. "KADMİYUMA MARUZ BIRAKILAN DİYABETİK RATLARA ALFA LİPOİK ASİT VE İNSÜLİN UYGULANMASININ OLASI DÜZENLEYİCİ ETKİLERİ". Afyon Kocatepe Üniversitesi Kocatepe Tıp Dergisi 19/3 (2018), 98-108. |
