The role of Blastocystis hominis in the activation of ulcerative colitis

HARMANDAR, Ferda; Çekin, Yeşim; uyar, seyit; ÇEKİN, Ayhan Hilmi; ŞAHİNTÜRK, Yasin; Kök, Mehmet

The role of Blastocystis hominis in the activation of ulcerative colitis

Mehmet KÖK, (Sağlık Bilimleri Üniversitesi Antalya Eğitim ve Araştırma Hastanesi, İç Hastalıkları Anabilim Dalı, Antalya, Türkiye)

Yeşim ÇEKİN, (Sağlık Bakanlığı, Antalya Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Kliniği, Antalya, Türkiye)

Ayhan Hilmi ÇEKİN, (Antalya Sağlık Bilimleri Üniversitesi, Antalya Eğitim ve Araştırma Hastanesi, Gastroenteroloji Bölümü, Antalya, Türkiye)

Seyit UYAR, (Sağlık Bilimleri Üniversitesi Antalya Eğitim ve Araştırma Hastanesi, İç Hastalıkları Anabilim Dalı, Antalya, Türkiye)

Ferda HARMANDAR, (Antalya Sağlık Bilimleri Üniversitesi, Antalya Eğitim ve Araştırma Hastanesi, Gastroenteroloji Bölümü, Antalya, Türkiye)

Yasin ŞAHİNTÜRK (Sağlık Bakanlığı, Antalya Eğitim ve Araştırma Hastanesi, İç Hastalıkları Bölümü, Antalya, Türkiye)

Turkish Journal of Gastroenterology

8 4

Yıl: 2019 Cilt: 30 Sayı: 1 Sayfa Aralığı: 40 - 46 Metin Dili: İngilizce İndeks Tarihi: 21-05-2019

The role of Blastocystis hominis in the activation of ulcerative colitis

Öz:

Background/Aims: Several studies have shown that a change in microbiota plays an important role in the pathogenesis of inflammatorybowel disease (IBD). Furthermore, with the emergence in recent studies of differences according to the subtype of IBD and whether thedisease is active or in remission, there has started to be research into the relationship between IBD and several microorganisms. Blastocystis hominis is primary among these organisms. The aim of the present study was to determine the role of B. hominis in the acuteflare-up of ulcerative colitis (UC).Materials and Methods: A total of 114 patients with UC were included in the study, with 52 in the active phase. The Mayo scoring systemwas used for the activity index. Patients determined with a flare-up agent other than B. hominis were excluded from the study. Fecalsamples of the patients were examined by the polymerase chain reaction method for the presence of B. hominis.Results: B. hominis positivity was determined in 37 (34%) patients with UC. Of the patients, 17 (32.6%) were in the acute flare-up phase,and 20 (32.2%) were in remission (p=0.961). In 11 (64.7%) of the B. hominis positive patients, the disease severity was determined asmild-moderate (p<0.001).Conclusion: The results of the present study showed that while there was no difference between the active and remission phases inrespect of B. hominis presence, there was milder involvement in those determined with B. hominis.

Anahtar Kelime:

Konular: Gastroenteroloji ve Hepatoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Dalal SR, Chang EB. The microbial basis of inflammatorybowel diseases. J Clin Invest 2014; 124: 4190-6. [CrossRef]
Frank DN, Amand ALS, Feldman RA, Boedeker EC, Harpaz N, Pace NR. Molecularphylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. Proc Natl Acad Sci U S A 2007; 104: 13780-5. [CrossRef]
Can G, Tezel A, Gürkan H, et al. Investigation of IL23R, JAK2, and STAT3 gene polymorphisms and gene-gene interactions in Crohn’s disease and ulcerative colitis in a Turkish population. Turk J Gastroenterol 2016; 27 : 525-36. [CrossRef]
Kostic AD, Xavier RJ, Gevers D. The microbiome in inflammatory bowel disease: current status and the future ahead. Gastroenterology 2014; 146: 1489-99. [CrossRef]
Kamada N, Seo SU, Chen GY, Núñez G. Role of the gut microbiota in immunity and inflammatory disease. Nat Rev Immunol 2013; 13: 321. [CrossRef]
Sellon RK, Tonkonogy S, Schultz M, et al. Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-1Odeficientmice. Infect Immun 1998; 66: 5224-31.
Onderdonk AB, Franklin ML, Cisneros RL. Production of experimental ulcerative colitis in gnotobiotic guinea pigs with simplified microflora. Infect Immun 1981; 32: 225-31.
Garrett WS, Lord GM, Punit S, et al. Communicable ulcerative colitis induced by T-bet deficiency in the innate immune system. Cell 2007; 131: 33-45. [CrossRef]
Claesson MJ, O’Sullivan O, Wang Q, et al. Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine. PLoS ONE 2009; 4: e6669. [CrossRef]
Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut microbiota. Nature 2012; 489: 220-30. [CrossRef]
Stensvold CR, Van Der Giezen M. Associations between Gut Microbiota and Common Luminal Intestinal Parasites. Trends Parasitol 2018; 34: 369-77. [CrossRef]
Stensvold CR, Nielsen HV, Molbak K, Smith HV. Pursuing the clinical significance of Blastocystis-diagnostic limitations. Trends Parasitol 2009; 25: 239. [CrossRef]
Cekin AH, Cekin Y, Adakan Y, Tasdemir E, Koclar FG, Yolcular BO. Blastocystosis in patients with gastrointestinal symptoms: A case-control study. BMC Gastroenterol 2012; 12: 122. [CrossRef]
Pascal V, Pozuelo M, Borruel N, et al. A microbial signature for Crohn’s disease. Gut 2017; 66: 813-22. [CrossRef]
Machiels K, Joossens M, Sabino J, et al. A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. Gut 2014; 63: 1275-83. [CrossRef]
Lepage P, Hasler R, Spehlmann ME, et al. Twin study indicates loss of interaction between microbiota and mucosa of patients with ulcerative colitis. Gastroenterology 2011; 141: 227-36. [CrossRef]
Pitcher MC, Beatty ER, Cummings JH. The contribution of sulphate reducing bacteria and 5-aminosalicylic acid to faecal sulphide in patients with ulcerative colitis. Gut 2000; 46: 64-72. [CrossRef]
Hold GL, Smith M, Grange C, Watt ER, El-Omar EM, Mukhopadhya I. Role of the gut microbiota in inflammatory bowel disease pathogenesis: What have we learnt in the past 10 years? World J Gastroenterol 2014; 20: 1192-210. [CrossRef]
Scanlan PD, Stensvold CR, Rajilić-Stojanović M, et al. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. FEMS Microbiol Ecol 2014; 90: 326- 30. [CrossRef]
Özyurt M, Kurt Ö, Molbak K, Nielsen HV, Haznedaroglu T, Stensvold CR. Molecular epidemiology of Blastocystis infections in Turkey. Parasitol Int 2008; 57: 300-6. [CrossRef]
Roberts T, Barratt J, Harkness J, Ellis J, Stark D. Comparison of microscopy,culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples. Am J Trop Med Hyg 2011; 84: 308-12. [CrossRef]
Stensvold CR, Arendrup MC, Jespersgaard C, Molbak K, Nielsen HV. Detecting Blastocystis using parasitologic and DNA-based methods: A comparative study. Diagn Microbiol Infect Dis 2007; 59: 303-7. [CrossRef]
Stensvold CR, Clark CG. Current status of Blastocystis: a personal view. Parasitol Int 2016; 65: 763-71. [CrossRef]
Long HY, Handschack A, Konig, Ambrosch A. Blastocytis hominis modulates immune responses and cytokine release in colonic epithelial cells. Parasitol Res 2001; 87: 1029-30.
Sio SW, Puthia MK, Lee AS, Lu J, Tan KS. Protease activity of Blastocystis hominis. Parasitol Res 2006; 99: 126-30. [CrossRef]
Roberts T, Stark D, Harkness J, Ellis J. Update on the pathogenic potential and treatment options for Blastocystis sp. Gut Pathog 2014; 6: 17. [CrossRef]
Yamamoto-Furusho JK, Torijano-Carrera E. Intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course. Digestion 2010; 82: 18-23. [CrossRef]
Termmathurapoj S, Leelayoova S, Aimpun P, et al. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitology Research 2004; 93: 445-47. [CrossRef]
Tai WP, Hu PJ, Wu J, Lin XC. Six ulcerative colitis patients with refractory symptoms coinfective with Blastocystis hominis in China. Parasitol Res 2011; 108: 1207-10. [CrossRef]
Coskun A, Malatyali E, Ertabaklar H, Yasar MB, Karaoglu AO, Ertug S. Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings. Asian Pac J Trop Med 2016; 9: 916-9. [CrossRef]
Poirier P, Wawrzyniak I, Albert A, El Alaoui H, Delbac F, Livrelli V. Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. Clin Microbiol 2011; 49: 975-83. [CrossRef]
Petersen AM, Stensvold CR, Mirsepasi H, et al. Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol 2013; 48: 638-9. [CrossRef]
Rossen NG, Bart A, Verhaar N, et al. Low prevalence of Blastocystis sp. in active ulcerative colitis patients. Eur J Clin Microbiol Infect Dis 2015; 34: 1039-44. [CrossRef]
Byndloss MX, Olsan EE, Rivera-Chavez F, et al. Microbiota-activated PPAR-γ signaling inhibits dysbiotic Enterobacteriaceae expansion. Science 2017; 357: 570-5. [CrossRef]
Winter SE, Baumler AJ. Why related bacterial species bloom simultaneously in the gut: Principles underlying the ‘Like will to like’ concept. Cell Microbiol 2014; 16: 179-84. [CrossRef]
Leung JM, Davenport M, Wolff MJ, et al. IL-22-producing C4+ cells are depleted in actively inflamed colitis tissue. Mucosal Immunol 2014; 7: 124-33. [CrossRef]

APA	Kök M, Çekin Y, ÇEKİN A, uyar s, HARMANDAR F, ŞAHİNTÜRK Y (2019). The role of Blastocystis hominis in the activation of ulcerative colitis. , 40 - 46.
Chicago	Kök Mehmet,Çekin Yeşim,ÇEKİN Ayhan Hilmi,uyar seyit,HARMANDAR Ferda,ŞAHİNTÜRK Yasin The role of Blastocystis hominis in the activation of ulcerative colitis. (2019): 40 - 46.
MLA	Kök Mehmet,Çekin Yeşim,ÇEKİN Ayhan Hilmi,uyar seyit,HARMANDAR Ferda,ŞAHİNTÜRK Yasin The role of Blastocystis hominis in the activation of ulcerative colitis. , 2019, ss.40 - 46.
AMA	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y The role of Blastocystis hominis in the activation of ulcerative colitis. . 2019; 40 - 46.
Vancouver	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y The role of Blastocystis hominis in the activation of ulcerative colitis. . 2019; 40 - 46.
IEEE	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y "The role of Blastocystis hominis in the activation of ulcerative colitis." , ss.40 - 46, 2019.
ISNAD	Kök, Mehmet vd. "The role of Blastocystis hominis in the activation of ulcerative colitis". (2019), 40-46.

APA	Kök M, Çekin Y, ÇEKİN A, uyar s, HARMANDAR F, ŞAHİNTÜRK Y (2019). The role of Blastocystis hominis in the activation of ulcerative colitis. Turkish Journal of Gastroenterology, 30(1), 40 - 46.
Chicago	Kök Mehmet,Çekin Yeşim,ÇEKİN Ayhan Hilmi,uyar seyit,HARMANDAR Ferda,ŞAHİNTÜRK Yasin The role of Blastocystis hominis in the activation of ulcerative colitis. Turkish Journal of Gastroenterology 30, no.1 (2019): 40 - 46.
MLA	Kök Mehmet,Çekin Yeşim,ÇEKİN Ayhan Hilmi,uyar seyit,HARMANDAR Ferda,ŞAHİNTÜRK Yasin The role of Blastocystis hominis in the activation of ulcerative colitis. Turkish Journal of Gastroenterology, vol.30, no.1, 2019, ss.40 - 46.
AMA	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y The role of Blastocystis hominis in the activation of ulcerative colitis. Turkish Journal of Gastroenterology. 2019; 30(1): 40 - 46.
Vancouver	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y The role of Blastocystis hominis in the activation of ulcerative colitis. Turkish Journal of Gastroenterology. 2019; 30(1): 40 - 46.
IEEE	Kök M,Çekin Y,ÇEKİN A,uyar s,HARMANDAR F,ŞAHİNTÜRK Y "The role of Blastocystis hominis in the activation of ulcerative colitis." Turkish Journal of Gastroenterology, 30, ss.40 - 46, 2019.
ISNAD	Kök, Mehmet vd. "The role of Blastocystis hominis in the activation of ulcerative colitis". Turkish Journal of Gastroenterology 30/1 (2019), 40-46.