Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials

TORLAK, Emrah; KÜRŞAT IŞIK, Mehmet

Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials

Emrah TORLAK, (Necmettin Erbakan University, Faculty of Science, Department of Molecular Biology and Genetics, Konya, Turkey)

Mehmet KÜRŞAT IŞIK (Konya Food and Agriculture University, Strategic Research and Development Centre, Konya, Turkey)

Etlik Veteriner Mikrobiyoloji Dergisi

2 0

Yıl: 2018 Cilt: 29 Sayı: 1 Sayfa Aralığı: 46 - 50 Metin Dili: İngilizce İndeks Tarihi: 27-05-2019

Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials

Öz:

American Foulbrood (AFB) is a highly contagious bacterial honey bee disease caused by Paenibacillus larvae. The elimination of P. larvae spores from contaminated hives is a key factor to achieve the long-term success in AFB control. The purpose of this study was to evaluate the efficacy of gaseous ozone in inactivating the P. larvae spores on wooden and plastic hive materials. Untreated pinewood and polyvinyl chloride (PVC) were chosen as representatives for the hive materials. Pinewood and PVC sticks inoculated with a spore cocktail of three P. larvae strains were treated up to 120 min with two different constant concentrations of gaseous ozone (9.8 and 17.1 mg/L) at room temperature. Ozonation at 17.1 mg/L for 120 min yielded over the 4 log reduction in the counts of spores on PVC sticks. Whereas, reduction of 2.3 log was obtained on pinewood sticks under the same experimental conditions. Reductions achieved in the levels of P. larvae spores on PVC sticks after 90 and 120 min of ozonation were significantly (p<0.05) higher than those on pinewood sticks. Our results suggest that gaseous ozone treatment is a promising candidate for the sterilization of plastic hives contaminated with P. larvae spores.

Anahtar Kelime:

Konular: Biyoloji Balıkçılık Mikrobiyoloji Viroloji Genetik ve Kalıtım Veterinerlik Zooloji

Ozon Gazının Kovan Materyalleri Üzerinde Paenibacillus Larvae Sporlarına Karşı Etkinliği

Öz:

Amerikan Yavru Çürüklüğü (AYÇ) Paenibacillus larvae’nın neden olduğu oldukça bulaşıcı bakteriyal bir bal arısı hastalığıdır. P. larvae sporlarının kontamine kovanlardan eliminasyonu AYÇ’nin uzun süreli başarılı kontrolünde anahtar bir faktördür. Bu çalışmanın amacı ozon gazının ahşap ve plastik kovan materyalleri üzerinde P. larvae sporlarını inaktivasyon etkinliğinin değerlendirilmesidir. Kovan materyallerini temsilen işlem görmemiş çam ağacı ve polivinil klorür (PVC) seçilmiştir. P. larvae sporları ile kontamine edilen çam ağacı ve PVC çubukları ozon gazı ile 120 dakikaya kadar iki farklı sabit konsantrasyonda (9.8 ve 17.1 mg/L) oda sıcaklığında muamele edilmiştir. PVC çubuklara 120 dakika boyunca 17.1 mg/L düzeyinde ozon uygulaması spor sayısında 4 log üzerinde azalmaya neden olmuştur. Buna karşılık, aynı deneysel koşullarda çam ağacı çubukları üzerinde 2.3 log azalma elde edilmiştir. PVC çubuklar üzerinde P. larvae spor seviyelerinde 90 ve 120 dakika ozonlama sonrasında elde edilen indirgenme değerleri çam ağacı çubukları üzerinde elde edilen değerlerden önemli (p<0.05) düzeyde yüksektir. Sonuçlarımız ozon gazı uygulamasının P. larvae sporlarıyla kontamine plastik kovanların sterilizasyonu için umut verici bir aday olduğunu düşündürmektedir.

Anahtar Kelime:

Konular: Biyoloji Balıkçılık Mikrobiyoloji Viroloji Genetik ve Kalıtım Veterinerlik Zooloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TORLAK E, KÜRŞAT IŞIK M (2018). Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. , 46 - 50.
Chicago	TORLAK Emrah,KÜRŞAT IŞIK Mehmet Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. (2018): 46 - 50.
MLA	TORLAK Emrah,KÜRŞAT IŞIK Mehmet Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. , 2018, ss.46 - 50.
AMA	TORLAK E,KÜRŞAT IŞIK M Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. . 2018; 46 - 50.
Vancouver	TORLAK E,KÜRŞAT IŞIK M Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. . 2018; 46 - 50.
IEEE	TORLAK E,KÜRŞAT IŞIK M "Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials." , ss.46 - 50, 2018.
ISNAD	TORLAK, Emrah - KÜRŞAT IŞIK, Mehmet. "Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials". (2018), 46-50.

APA	TORLAK E, KÜRŞAT IŞIK M (2018). Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. Etlik Veteriner Mikrobiyoloji Dergisi, 29(1), 46 - 50.
Chicago	TORLAK Emrah,KÜRŞAT IŞIK Mehmet Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. Etlik Veteriner Mikrobiyoloji Dergisi 29, no.1 (2018): 46 - 50.
MLA	TORLAK Emrah,KÜRŞAT IŞIK Mehmet Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. Etlik Veteriner Mikrobiyoloji Dergisi, vol.29, no.1, 2018, ss.46 - 50.
AMA	TORLAK E,KÜRŞAT IŞIK M Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. Etlik Veteriner Mikrobiyoloji Dergisi. 2018; 29(1): 46 - 50.
Vancouver	TORLAK E,KÜRŞAT IŞIK M Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials. Etlik Veteriner Mikrobiyoloji Dergisi. 2018; 29(1): 46 - 50.
IEEE	TORLAK E,KÜRŞAT IŞIK M "Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials." Etlik Veteriner Mikrobiyoloji Dergisi, 29, ss.46 - 50, 2018.
ISNAD	TORLAK, Emrah - KÜRŞAT IŞIK, Mehmet. "Efficacy of Gaseous Ozone Against Paenibacillus Larvae Spores on Hive Materials". Etlik Veteriner Mikrobiyoloji Dergisi 29/1 (2018), 46-50.